Enhancement of drought tolerance in diverse Vicia faba cultivars by inoculation with plant growth-promoting rhizobacteria under newly reclaimed soil conditions

AbstractWater deficit has devastating impacts on legume production, particularly with the current abrupt climate changes in arid environments. The application of plant growth-promoting rhizobacteria (PGPR) is an effective approach for producing natural nitrogen and attenuating the detrimental effects of drought stress. This study investigated the influence of inoculation with the PGPR Rhizobium leguminosarum biovar viciae (USDA 2435) and Pseudomonas putida (RA MTCC5279) solely or in combination on the physio-biochemical and agronomic traits of five diverse Vicia faba cultivars under well-watered (100% crop evapotranspiration [ETc]), moderate drought (75% ETc), and severe drought (50% ETc) conditions in newly reclaimed poor-fertility sandy soil. Drought stress substantially reduced the expression of photosynthetic pigments and water relation parameters. In contrast, antioxidant enzyme activities and osmoprotectants were considerably increased in plants under drought stress compared with those in well-watered plants. These adverse effects of drought stress reduced crop water productivity (CWP) and seed yield‐related traits. However, the application of PGPR, particularly a consortium of both strains, improved these parameters and increased seed yield and CWP. The evaluated cultivars displayed varied tolerance to drought stress: Giza-843 and Giza-716 had the highest tolerance under well-watered and moderate drought conditions, whereas Giza-843 and Sakha-4 were more tolerant under severe drought conditions. Thus, co-inoculation of drought-tolerant cultivars with R. leguminosarum and P. putida enhanced their tolerance and increased their yield and CWP under water-deficit stress conditions. This study showed for the first time that the combined use of R. leguminosarum and P. putida is a promising and ecofriendly strategy for increasing drought tolerance in legume crops.

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Interactive Effects of Plant Growth-Promoting Rhizobacteria and Phosphates Sources on Growth and Phosphorus Nutrition of Soybean under Moderate Drought

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47311371 ◽

2019 ◽

Vol 47 (3) ◽

Author(s):

Vladimir Ion ROTARU ◽

Luxita RISNOVEANU

Keyword(s):

Drought Stress ◽

Plant Growth ◽

Water Supply ◽

Nutrient Uptake ◽

Burkholderia Cepacia ◽

Plant Growth Promoting Rhizobacteria ◽

P Nutrition ◽

Moderate Drought ◽

Plant Growth Promoting ◽

Growth Promoting

The growth of legume plants is usually improved by the rhizobacteria inoculation under low phosphorus (P) and alleviation of P nutrition plays important role in plant drought stress response. The aim of this study was to assess the comparative efficacy of two plant growth promoting rhizobacteria namely Burkholderia cepacia B36 and Enterobacter radicincitans D5/23T combined with two sources of phosphates in soybean (Glycine max L.) under low water supply. Plants were grown under P soluble versus insoluble P fertilization for comparing the effects of soybean inoculation on growth, uptake and use efficiency of phosphorus under moderate drought stress. At the beginning of flowering, half of plants was subjected to low water supply (35% water holding capacity, WHC) for 12 days while control plants were well watered - 70% WHC. The plants were harvested at the end of drought and physiological traits and P contents were analyzed. The inoculation treatments showed better plant growth and nutrient uptake when compared to uninoculated control. The application of the Burkholderia cepacia was more efficiently in terms plant growth than E. radicincitans especially under insoluble phosphates. Phosphorus concentrations of shoots and roots increased with both bacterial strains. The bacterial inoculation has much better stimulatory effect on nutrient uptake by soybean fertilized with insoluble phosphates. Study findings indicate that the combined application of PGPR (Burkholderia cepacia B36) and P amendments has the potential to improve P nutrition and growth of soybean cultivated on P-deficient soil under well-watered as well as moderate drought condition. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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Plant Growth Promoting Rhizobacterial Mitigation of Drought Stress in Crop Plants: Implications for Sustainable Agriculture

Agronomy ◽

10.3390/agronomy9110712 ◽

2019 ◽

Vol 9 (11) ◽

pp. 712 ◽

Cited By ~ 10

Author(s):

Omena Ojuederie ◽

Oluwaseyi Olanrewaju ◽

Olubukola Babalola

Keyword(s):

Drought Stress ◽

Plant Growth ◽

Acc Deaminase ◽

Plant Growth Promoting Rhizobacteria ◽

Crop Growth ◽

Growth And Yield ◽

Growth Enhancement ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Effects Of Drought

Abiotic stresses arising from climate change negates crop growth and yield, leading to food insecurity. Drought causes oxidative stress on plants, arising from excessive production of reactive oxygen species (ROS) due to inadequate CO2, which disrupts the photosynthetic machinery of plants. The use of conventional methods for the development of drought-tolerant crops is time-consuming, and the full adoption of modern biotechnology for crop enhancement is still regarded with prudence. Plant growth-promoting rhizobacteria (PGPR) could be used as an inexpensive and environmentally friendly approach for enhancing crop growth under environmental stress. The various direct and indirect mechanisms used for plant growth enhancement by PGPR were discussed. Synthesis of 1-aminocyclopropane−1-carboxylate (ACC) deaminase enhances plant nutrient uptake by breaking down plant ACC, thereby preventing ethylene accumulation, and enable plants to tolerate water stress. The exopolysaccharides produced also improves the ability of the soil to withhold water. PGPR enhances osmolyte production, which is effective in reducing the detrimental effects of ROS. Multifaceted PGPRs are potential candidates for biofertilizer production to lessen the detrimental effects of drought stress on crops cultivated in arid regions. This review proffered ways of augmenting their efficacy as bio-inoculants under field conditions and highlighted future prospects for sustainable agricultural productivity.

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ACC-deaminase producing plant growth promoting rhizobacteria and biochar mitigate adverse effects of drought stress on maize growth

PLoS ONE ◽

10.1371/journal.pone.0230615 ◽

2020 ◽

Vol 15 (4) ◽

pp. e0230615 ◽

Cited By ~ 14

Author(s):

Subhan Danish ◽

Muhammad Zafar-ul-Hye ◽

Fauzia Mohsin ◽

Mubshar Hussain

Keyword(s):

Drought Stress ◽

Adverse Effects ◽

Plant Growth ◽

Acc Deaminase ◽

Plant Growth Promoting Rhizobacteria ◽

Maize Growth ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Effects Of Drought

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Effect of plant growth promoting rhizobacteria (PGPR) and water stress on phytohormones and polyamines of soybean

Indian Journal of Agricultural Research ◽

10.18805/ijare.v49i5.5805 ◽

2015 ◽

Vol 49 (5) ◽

Cited By ~ 3

Author(s):

Hossein Zahedi ◽

Samira Abbasi

Keyword(s):

Abscisic Acid ◽

Drought Stress ◽

Plant Growth ◽

Plant Growth Promoting Rhizobacteria ◽

Control Treatment ◽

Rhizobium Japonicum ◽

Severe Drought ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Soybean Plants

The effect of inoculation of three plant growth promoting rhizobacteria (PGPR) that is <italic>Rhizobium japonicum</italic>, <italic>Azotobacter chroococcum</italic> and <italic>Azospirillum brasilense</italic> and mixture of them on phytohormones and polyamines of soybean under different irrigation regimes was investigated. Drought stress induced by irrigation withholding until 40, 80 and 120 mm evaporation from evaporation pan. However seed bacterization of soybean was accompanied with 20 kg ha−1 nitrogen. In addition, 20 and 100 kg ha−1 nitrogen were considered as control treatments. The results showed that drought stress significantly decreased cytokinin, gibberellin and auxin accumulation in plant tissues. By contrast, drought stress led to increase in abscisic acid accumulation in soybean plants. Polyamines that are putrescine and spermidine increased due to drought stress and then decreased under severe drought stress. PGPR application had positive effect on growth promoting phytohormones compared to control treatment. However the highest accumulation of cytokinin, gibberellin and auxin was related to 100 kg ha−1 nitrogen treatment. In case of abscisic acid PGPR application decreased its accumulation. Asignificant decrease as observed on polyamines accumulation when PGPRs were applied on stressed soybean plants.

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