Evaluation of Rapid Blood Sample Collection in the Detection of Circulating Filarial Antigens for Epidemiological Survey by rWbSXP-1 Capture Assay

AbstractThe effect of method of blood sample collection (automatic blood sampling equipment (ABSE) v. manual) on cortisol and progesterone concentrations was investigated in 20 farmed red deer hinds and 20 domestic sheep ewes following dexamethasone and exogenous ACTH administration. Ten animals were subjected to either automatic sampling or manual sampling via jugular venipuncture in 1 week, with the treatment groups reversed in the 2nd week. The ABSE was programmed to collect a blood sample, then deliver 2 mg dexamethasone, collect a further blood sample 120 min later and then inject 100 fig ACTH. Thereafter, samples were collected at 15-min intervals during a 2·5 h period (12 samples in total). In the manual injection and sampling treatment, four samples were collected: (1) before dexamethasone administration, (2) before ACTH administration, (3) 60 min after ACTH administration, and (4) 150 min after ACTH administration. The success rate of blood sampling with ABSE was 80%. The overall mean packed cell volume (PCV) from samples collected by ABSE from both hinds and ewes was significantly lower than that from samples collected manually (P < 0·01) and PCV declined with time in manually sampled animals (P < 0·01). Plasma cortisol concentrations peaked at 45 min after ACTH administration in sheep and deer. In sheep, there was a marked fluctuation in the plasma cortisol concentrations with time. Both deer and sheep showed a reduced cortisol response to ACTH during week 2 irrespective of sampling method suggesting down-regulation of the response to ACTH. Maximum mean plasma progesterone concentration was reached at 15 to 30 min after ACTH administration. No significant differences in cortisol and progesterone responses due to blood sampling method were found in animals receiving prior dexamethasone treatment. This demonstrates that the ABSE has the ability to be used to effectively conduct ACTH stimulation tests without the need to handle the animals during the test.

Download Full-text

On the improvement of blood sample collection at clinical laboratories

BMC Health Services Research ◽

10.1186/1472-6963-14-12 ◽

2014 ◽

Vol 14 (1) ◽

Cited By ~ 25

Author(s):

Alex Grasas ◽

Helena Ramalhinho ◽

Luciana S Pessoa ◽

Mauricio GC Resende ◽

Imma Caballé ◽

...

Keyword(s):

Blood Sample ◽

Sample Collection ◽

Clinical Laboratories

Download Full-text

Changing the Time of Blood Collection to Determine Vancomycin Concentrations in Intensive Care Unit Patients

Critical Care Nurse ◽

10.4037/ccn2018594 ◽

2018 ◽

Vol 38 (1) ◽

pp. 24-28

Author(s):

Drayton A. Hammond ◽

Taylor B. James ◽

Lexis N. Atkinson ◽

Jacob T. Painter ◽

Katherine Lusardi

Keyword(s):

Intensive Care Unit ◽

Intensive Care ◽

Blood Sample ◽

Medical Intensive Care Unit ◽

Sample Collection ◽

Blood Samples ◽

Significant Difference ◽

Vancomycin Trough ◽

Medical Intensive Care ◽

Trough Levels

BACKGROUND Clinical practice guidelines for initiation and therapeutic drug monitoring, but not timing, of vancomycin dosing exist at many institutions. Scheduling vancomycin trough measurements and doses around the morning blood sample collection could yield more interpretable troughs and increase patient safety. OBJECTIVE To evaluate the appropriateness of blood sample collection times for vancomycin trough measurements before and after an initiative to change the timing of blood sampling to determine vancomycin doses and trough levels in a medical intensive care unit. METHODS A retrospective cohort study was conducted of patients in a medical intensive care unit who received intravenous vancomycin at a scheduled interval. Differences in continuous and categorical data were compared between pre- and postintervention groups. The primary outcome was proportion of blood samples collected for vancomycin trough measurements within 30 minutes of the next scheduled vancomycin dose. RESULTS Baseline characteristics were similar between the preintervention (n = 68) and postintervention (n = 176) groups except for the percentage of blood samples drawn for trough measurements and morning laboratory tests (6% vs 81%; P < .001). Frequency of loading doses was similar between patients in the pre- and postintervention groups, as was weight-based maintenance dosing. There was no significant difference in the percentage of blood samples collected to measure vancomycin trough levels appropriately at 30, 60, or 75 minutes from the next scheduled dose. CONCLUSION Measuring vancomycin trough levels in morning blood samples did not affect the percentage of inappropriately collected blood samples used to measure vancomycin trough levels.

Download Full-text

Appendix 2: Anticoagulants Commonly Used in Blood Sample Collection

Handbook of LC-MS Bioanalysis ◽

10.1002/9781118671276.app2 ◽

2013 ◽

pp. 661-661

Keyword(s):

Blood Sample ◽

Sample Collection

Download Full-text

9 Blood Sample Collection and Preparation in Birds

Exotic Animal Hematology and Cytology ◽

10.1002/9781118993705.ch9 ◽

2015 ◽

pp. 165-172

Keyword(s):

Blood Sample ◽

Sample Collection

Download Full-text

Impact of blood sample collection methods on blood protein profiling studies

Clinica Chimica Acta ◽

10.1016/j.cca.2017.05.030 ◽

2017 ◽

Vol 471 ◽

pp. 128-134 ◽

Cited By ~ 11

Author(s):

Maria Ilies ◽

Cristina Adela Iuga ◽

Felicia Loghin ◽

Vishnu Mukund Dhople ◽

Thomas Thiele ◽

...

Keyword(s):

Blood Sample ◽

Protein Profiling ◽

Blood Protein ◽

Sample Collection ◽

Collection Methods

Download Full-text

Nurses’ Experiences of Blood Sample Collection from Children: A Qualitative Study from Swedish Paediatric Hospital Care

10.21203/rs.3.rs-144185/v1 ◽

2021 ◽

Author(s):

Henrik Hjelmgren ◽

Britt-Marie Ygge ◽

Björn Nordlund ◽

Nina Andersson

Keyword(s):

Focus Group ◽

Qualitative Study ◽

Blood Sample ◽

Hospital Care ◽

Blood Sampling ◽

Sample Collection ◽

Focus Group Interviews ◽

Paediatric Hospital ◽

Group Interviews ◽

Analytical Errors

Abstract Background: Blood sampling collections are necessary and important for diagnosis and treatment in paediatric hospital care. Nurses play an active role in helping the children with the blood sampling experience. Unfortunately, the blood sampling collection procedure is often affected by pre-analytical errors, leading to consequences such as delayed diagnosis, treatment and hospital stay, as well as repeated sampling. Moreover, children state that needle procedures are the worst experience of their hospital stay. Nurses working in children’s hospitals are responsible for conducting most of the needle related procedures but their experience of errors occurring during blood sample collection is unknown. The aim of this study therefore was to describe paediatric nurses’ experiences of blood sampling collections from children. Method: We used a qualitative study design with a (reflexive) thematic analysis (TA) method. Three focus group interviews were conducted, with 19 nurses from Sweden working at two different paediatric hospitals, focusing on their experiences of the blood sample collection procedure. Results: From the three focus group interviews we analysed patterns and meanings of the following themes: Paediatric blood sampling is a challenge for the nurses, Nurses’ feelings of frustration with unsuccessful samplings, Nurses believe in team work, Venous blood sampling was experienced as the best option, and Nurses’ thoughts and needs regarding skills development in paediatric blood sampling. Conclusion: The narrative results of this study illustrate that nurses working in paediatric hospital care face a big challenge in blood sampling collection from children. The nurses felt frustrated due to unsuccessful blood samplings and frequently could not understand why pre-analytical errors occurred. Nevertheless, they felt strengthened by colleagues in their team and shared feelings of responsibility to help each other with this complex procedure. The implications of this study are that paediatric hospital care needs to focus on improving guidelines for and increasing competence in blood sampling children and helping nurses to understand why samplings may be unsuccessful and how this can be avoided.

Download Full-text