Study objectives: To build a database recording all types of spoligotyping genes to serve the purpose of analyzing the result of spoligotyping technique in the study of the genetic diversity of Mycobacteria tuberculosis complex. Object and Methods: To use SpolDB4 as the reference database from Guadeloupe Paster Institute to employ PHP language for programming and record the spoligotyping data in MySQL. The module is run on the web platform. To build statistical functions according to different data entries and different searching tools according to various data input. Results: To complete the building of “SpoligoDB ver4” database to meet the demand of fast, visual and exact searching requirements and to fulfill the result testing process on spoligotyping technique among researches on classifications and drug-resistance and of Mycobacterium tuberculosis complex at Carlo Urbani Center, Hue University of Medicine and Pharmacy and other agencies at the following address http://khdn-yhue.vn/modules.php?name=Spoligodb; http://carlo-urbani-center.org/en/modules.php?name=Spoligodb. Conclusion: To complete the building of SpologoDB ver4 database from enucleated SpolDB4 database provided by the Guadeloupe Pasteur Institute and meet its requirements to serve the result analysis process on spoligotyping technique for those units that wish to research on the genetic diversity of Mycobacterium tuberculosis complex. Keywords: spoligotyping, spoligotype pattern, SpoligoDB ver4, AIE-VNM.
Molecular Typing of Mycobacterium Tuberculosis Complex by 24-Locus Based MIRU-VNTR Typing in Conjunction with Spoligotyping to Assess Genetic Diversity of Strains Circulating in Morocco
