Abstract
Though cry gene transformed crops have successfully revolutionized modern agriculture, it is still necessary to discover new Cry proteins to overcome potential threatens from the development of resistant insect populations. We swapped domain-IIIs with various Cry proteins and engineered seven chimeric proteins, aiming to produce new engineered hybrid insecticidal proteins. Seven recombinant proteins were expressed in Escherichia coli. Three proteins exhibited high toxicity against Asian corn borer in dietary exposure assays. Three hybrid proteins were further transformed to rice (cv. Jijing88) to determine their insecticidal activity. Cry1Ab/Gc hybrid proteins, Cry1Ab being replaced by the domain-III of Cry1Gc, showed significantly more toxic against rice stem borer than others. Furthermore, Cry1Ab/Gc gene was transformed into maize (cv. HiII), then backcrossed into commercial maize inbred lines (cv. Ji853 and Y822), and formulated into Xiangyu 998 hybrid to evaluate their commercial value. Transgenic maize performed significant resistance improvement to the Asian corn borer without affecting the yield, and this new protein did not have adverse effects on the environment. Our result proved domain-swapped could be used as an efficient method for exploring new cry genes and engineered hybrid insecticidal protein. Cry1Ab/Gc provides a new tool for Lepidopteran insects resistant management in rice and maize.
Domain III of Bacillus thuringiensis Cry1Ie Toxin Plays an Important Role in Binding to Peritrophic Membrane of Asian Corn Borer
