Ran Involved in the Development and Reproduction Is a Potential Target for RNA-Interference-Based Pest Management in Nilaparvata lugens

AbstractDouble-stranded RNAs (dsRNAs) represent a promising class of biosafe insecticidal compounds. We examined the ability to induce RNA interference (RNAi) in the pollen beetle Brassicogethes aeneus via anther feeding, and compared short-term (3 d) to chronic (17 d) feeding of various concentrations of dsRNA targeting αCOP (dsαCOP). In short-term dsαCOP feeding, only the highest concentration resulted in significant reductions in B. aeneus survival; whereas in chronic dsαCOP feeding, all three concentrations resulted in significant mortality. Chronic dsαCOP feeding also resulted in significantly greater mortality compared to short-term feeding of equivalent dsαCOP concentrations. Our results have implications for the economics and development of dsRNA spray approaches for managing crop pests, in that multiple lower-concentration dsRNA spray treatments across crop growth stages may result in greater pest management efficacy, compared to single treatments using higher dsRNA concentrations. Furthermore, our results highlight the need for research into the development of RNAi cultivars for oilseed rape protection, given the enhanced RNAi efficacy resulting from chronic, compared to short-term, dsRNA feeding in B. aeneus.

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Advance towards Host Mediated RNA Interference Insect Pest Management

Advances in Biotechnology & Microbiology ◽

10.19080/aibm.2017.05.555666 ◽

2017 ◽

Vol 5 (4) ◽

Author(s):

Jagdish Jaba

Keyword(s):

Rna Interference ◽

Pest Management ◽

Insect Pest ◽

Insect Pest Management

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Effect of hepatocyte nuclear factor 4 on the fecundity of Nilaparvata lugens: Insights from RNA interference combined with transcriptomic analysis

Genomics ◽

10.1016/j.ygeno.2020.08.002 ◽

2020 ◽

Vol 112 (6) ◽

pp. 4585-4594 ◽

Cited By ~ 2

Author(s):

Yibei Cheng ◽

Yimin Li ◽

Wenru Li ◽

Yuanyuan Song ◽

Rensen Zeng ◽

...

Keyword(s):

Rna Interference ◽

Nilaparvata Lugens ◽

Transcriptomic Analysis ◽

Nuclear Factor ◽

Hepatocyte Nuclear Factor ◽

Hepatocyte Nuclear Factor 4

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Mechanisms, Applications, and Challenges of Insect RNA Interference

Annual Review of Entomology ◽

10.1146/annurev-ento-011019-025224 ◽

2020 ◽

Vol 65 (1) ◽

pp. 293-311 ◽

Cited By ~ 44

Author(s):

Kun Yan Zhu ◽

Subba Reddy Palli

Keyword(s):

Rna Interference ◽

Pest Management ◽

New Technologies ◽

Insect Pest ◽

Management Strategies ◽

Cationic Liposome ◽

Delivery Methods ◽

Nontarget Effects ◽

Development Of Resistance ◽

Rnai Efficiency

The RNA interference (RNAi) triggered by short/small interfering RNA (siRNA) was discovered in nematodes and found to function in most living organisms. RNAi has been widely used as a research tool to study gene functions and has shown great potential for the development of novel pest management strategies. RNAi is highly efficient and systemic in coleopterans but highly variable or inefficient in many other insects. Differences in double-stranded RNA (dsRNA) degradation, cellular uptake, inter- and intracellular transports, processing of dsRNA to siRNA, and RNA-induced silencing complex formation influence RNAi efficiency. The basic dsRNA delivery methods include microinjection, feeding, and soaking. To improve dsRNA delivery, various new technologies, including cationic liposome–assisted, nanoparticle-enabled, symbiont-mediated, and plant-mediated deliveries, have been developed. Major challenges to widespread use of RNAi in insect pest management include variable RNAi efficiency among insects, lack of reliable dsRNA delivery methods, off-target and nontarget effects, and potential development of resistance in insect populations.

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