scholarly journals A Single 9-Colour Flow Cytometric Method to Characterise Major Leukocyte Populations in the Rat: Validation in a Model of LPS-Induced Pulmonary Inflammation

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0142520 ◽  
Author(s):  
Ashton Barnett-Vanes ◽  
Anna Sharrock ◽  
Mark A. Birrell ◽  
Sara Rankin
2013 ◽  
Vol 14 (3) ◽  
pp. 249-259 ◽  
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Bianca Rovati ◽  
Sara Mariucci ◽  
Rossella Poma ◽  
Carmine Tinelli ◽  
Sara Delfanti ◽  
...  

2007 ◽  
Vol 323 (2) ◽  
pp. 160-171 ◽  
Author(s):  
Marguerite Bracher ◽  
Hannah J. Gould ◽  
Brian J. Sutton ◽  
David Dombrowicz ◽  
Sophia N. Karagiannis

1997 ◽  
Vol 202 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Michael A Model ◽  
Mark A KuKuruga ◽  
Robert F Todd

1997 ◽  
Vol 97 (1) ◽  
pp. 141-145 ◽  
Author(s):  
E. C. M. Hendriks ◽  
A. J. M. De Man ◽  
Y. C. M. Van Berkel ◽  
S. Stienstra ◽  
T. De Witte

2006 ◽  
Vol 315 (1-2) ◽  
pp. 1-10 ◽  
Author(s):  
Wannee Kantakamalakul ◽  
Kovit Pattanapanyasat ◽  
Surat Jongrakthaitae ◽  
Vatcharain Assawadarachai ◽  
Silawun Ampol ◽  
...  

2017 ◽  
Vol 92 (6) ◽  
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Julien Guy ◽  
Orianne Wagner-Ballon ◽  
Olivier Pages ◽  
François Bailly ◽  
Jessica Borgeot ◽  
...  

2010 ◽  
Vol 77A (11) ◽  
pp. 1091-1097 ◽  
Author(s):  
Alice Vines ◽  
Gethin J. McBean ◽  
Alfonso Blanco-Fernández

2005 ◽  
Vol 2005 (3) ◽  
pp. 232-237 ◽  
Author(s):  
Saurabh Singh ◽  
Vasker Bhattacherjee ◽  
Partha Mukhopadhyay ◽  
Christopher A. Worth ◽  
Samuel R. Wellhausen ◽  
...  

During the early stages of embryogenesis, pluripotent neural crest cells (NCC) are known to migrate from the neural folds to populate multiple target sites in the embryo where they differentiate into various derivatives, including cartilage, bone, connective tissue, melanocytes, glia, and neurons of the peripheral nervous system. The ability to obtain pure NCC populations is essential to enable molecular analyses of neural crest induction, migration, and/or differentiation. CrossingWnt1-CreandZ/EGtransgenic mouse lines resulted in offspring in which theWnt1-Cretransgene activated permanent EGFP expression only in NCC. The present report demonstrates a flow cytometric method to sort and isolate populations of EGFP-labeled NCC. The identity of the sorted neural crest cells was confirmed by assaying expression of known marker genes by TaqMan Quantitative Real-Time Polymerase Chain Reaction (QRT-PCR). The molecular strategy described in this report provides a means to extract intact RNA from a pure population of NCC thus enabling analysis of gene expression in a defined population of embryonic precursor cells critical to development.


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