scholarly journals Synthesis of a precursor of D-fagomine by immobilized fructose-6-phosphate aldolase

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250513
Author(s):  
Gerard Masdeu ◽  
Luis Miguel Vázquez ◽  
Josep López-Santín ◽  
Gloria Caminal ◽  
Slavko Kralj ◽  
...  
Keyword(s):  
Magnetic Nanoparticle ◽  
Reaction Rate ◽  
Immobilized Enzyme ◽  
Conversion Yield ◽  
Bond Forming ◽  
Nanoparticle Clusters ◽  
Reaction Cycles ◽  
Rate Conversion ◽  
Important Enzyme ◽  
High Reaction Rate

Fructose-6-phosphate aldolase (FSA) is an important enzyme for the C-C bond-forming reactions in organic synthesis. The present work is focused on the synthesis of a precursor of D-fagomine catalyzed by a mutant FSA. The biocatalyst has been immobilized onto several supports: magnetic nanoparticle clusters (mNC), cobalt-chelated agarose (Co-IDA), amino-functionalized agarose (MANA-agarose) and glyoxal-agarose, obtaining a 29.0%, 93.8%, 89.7% and 53.9% of retained activity, respectively. Glyoxal-agarose FSA derivative stood up as the best option for the synthesis of the precursor of D-fagomine due to the high reaction rate, conversion, yield and operational stability achieved. FSA immobilized in glyoxal-agarose could be reused up to 6 reaction cycles reaching a 4-fold improvement in biocatalyst yield compared to the non-immobilized enzyme.

Analysis of NiMoP/γ-Al2O3 Catalyst Preparation with Impregnation and Microwave Polyol Methods for Bio-Jet Production

2020 ◽  
Vol 1000 ◽  
pp. 257-264
Author(s):  
Bambang Heru Susanto ◽  
Joshua Raymond Valentino Siallagan
Keyword(s):  
Coconut Oil ◽  
Catalyst Preparation ◽  
Operating Conditions ◽  
Catalyst Loading ◽  
Impregnation Method ◽  
Conversion Yield ◽  
Jet Production ◽  
Cracking Process ◽  
Catalyst Impregnation ◽  
Rate Conversion

Bio-Jet could be produced by the synthesis of vegetable oil through the hydrodeoxygenation, decarboxylation, decarbonization, and catalytic cracking process. Physical characteristics, activities, and selectivity of the catalyst used will determine the rate, conversion, and yield of the reaction that being carried out. This study aims to compare and obtain the best characteristics of NiMoP/γ-Al2O3 catalysts synthesized using two types of preparation, impregnation and microwave polyol methods, which will be used for bio-jet production. The impregnation method takes more than 24 hours for catalyst preparation, while microwave polyols that use microwaves can synthesize catalysts faster. Both catalysts have almost the same loading on the weight of the catalyst, which in the microwave polyol method has a more dispersed promotor and active site, although the crystallinity level is deficient and tends to be amorphous compared to the impregnation method with high crystallinity. In bio-jet synthesis reaction with operating conditions of 5% catalyst loading by comparison to Coconut Oil, 400°C, and 15 bar, the conversion, yield, and selectivity of catalyst impregnation were 91.705%, 47.639%, and 84.511%, while microwave polyol catalysts were 90.296%, 42.752%, and 82.517%, respectively. In conclusion, microwave polyol provides a more effective and efficient preparation method.

Simulated Clustering Dynamics of Colloidal Magnetic Nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Frederik Laust Durhuus ◽  
Lau Halkier Wandall ◽  
Mathias Hoeg Boisen ◽  
Mathias Kure ◽  
Marco Beleggia ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Self Assembly ◽  
Biomedical Applications ◽  
Magnetic Nanoparticle ◽  
Bottom Up ◽  
Novel Materials ◽  
Nanoparticle Clusters ◽  
Clustering Dynamics

Magnetically guided self-assembly of nanoparticles is a promising bottom-up method to fabricate novel materials and superstructures, such as, for example, magnetic nanoparticle clusters for biomedical applications. The existence of assembled...

scholarly journals Detection of Salmonella bacteria in milk using gold-coated magnetic nanoparticle clusters and lateral flow filters

RSC Advances ◽  
2016 ◽  
Vol 6 (54) ◽  
pp. 48445-48448 ◽  
Author(s):  
Jeongin Hwang ◽  
Donghoon Kwon ◽  
Sanghee Lee ◽  
Sangmin Jeon
Keyword(s):  
Magnetic Nanoparticle ◽  
Lateral Flow ◽  
Nanoparticle Clusters ◽  
Novel Method

A novel method was developed for the detection of Salmonella bacteria using gold-coated magnetic nanoparticle clusters (Au/MNCs) and lateral flow filters.

scholarly journals Fabrication of Colorimetric Textile Sensor Based on Rhodamine Dye for Acidic Gas Detection

Polymers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 431 ◽  
Author(s):  
Young Ki Park ◽  
Byeong M. Oh ◽  
A Ra Jo ◽  
Ji Hyeon Han ◽  
Jee Young Lim ◽  
...  
Keyword(s):  
Reaction Rate ◽  
Color Change ◽  
Ph Sensitivity ◽  
Gas Detection ◽  
Textile Sensors ◽  
Printed Sensors ◽  
Textile Sensor ◽  
Rhodamine Dye ◽  
Strong Acids ◽  
High Reaction Rate

For the immediate detection of gaseous strong acids, it is advantageous to employ colorimetric textile sensors based on halochromic dyes. Thus, a rhodamine dye with superior pH sensitivity and high thermal stability was synthesized and incorporated in nylon 6 and polyester fabrics to fabricate textile sensors through dyeing and printing methods. The spectral properties and solubility of the dye were examined; sensitivity to acidic gas as well as durability and reversibility of the fabricated textile sensors were investigated. Both dyed and printed sensors exhibited a high reaction rate and distinctive color change under the acidic condition owing to the high pH sensitivity of the dye. In addition, both sensors have outstanding durability and reversibility after washing and drying.

scholarly journals Syncatalytic inactivation of prolyl 4-hydroxylase by anthracyclines

1988 ◽  
Vol 251 (2) ◽  
pp. 365-372 ◽  
Author(s):  
V Günzler ◽  
H M Hanauske-Abel ◽  
R Myllylä ◽  
D D Kaska ◽  
A Hanauske ◽  
...  
Keyword(s):  
Reaction Rate ◽  
Total Radioactivity ◽  
Human Origin ◽  
Iron Ion ◽  
First Order ◽  
Irreversible Inhibitors ◽  
Semiquinone Radicals ◽  
Enzyme Subunits ◽  
High Concentrations ◽  
Reaction Cycles

The anthracyclines doxorubicin and daunorubicin were found to act as irreversible inhibitors of prolyl 4-hydroxylase. The reaction rate for enzyme from both chick and human origin was first order, the concentration of inhibitor giving 50% inhibition being 60 microM for both compounds after 1 h. The effect was dependent on the presence of iron ions in the reaction mixture. Inactivation could be prevented by addition of high concentrations of ascorbate, but not 2-oxoglutarate, before the inactivation period. The same results were obtained with competitive analogues of these cosubstrates. Lysyl hydroxylase from chick embryos was also susceptible to inactivation. Its activity was decreased by 50% after incubation for 1 h with a 150 microM concentration of the inhibitors. When chick-embryo prolyl 4-hydroxylase was incubated with [14-14C]doxorubicin, both enzyme subunits were radioactively labelled, about 70% of the total radioactivity being found in the alpha-subunit. Since the anthracyclines are known to undergo a redox reaction generating semiquinone radicals with Fe3+ only, the results suggest that the enzyme-bound iron ion is oxidized to a tervalent intermediate in uncoupled reaction cycles. The data also suggest that both enzyme subunits contribute to the catalytic site of prolyl 4-hydroxylase.

Development and Potential Theranostic Applications of a Self-Assembled Hybrid of Magnetic Nanoparticle Clusters with Polysaccharide Nanogels

ChemPlusChem ◽  
2014 ◽  
Vol 79 (11) ◽  
pp. 1631-1637 ◽  
Author(s):  
Kiyofumi Katagiri ◽  
Keiko Ohta ◽  
Kaori Sako ◽  
Kei Inumaru ◽  
Koichiro Hayashi ◽  
...  
Keyword(s):  
Magnetic Nanoparticle ◽  
Nanoparticle Clusters ◽  
Self Assembled ◽  
Theranostic Applications

Colorimetric detection of pathogenic bacteria using platinum-coated magnetic nanoparticle clusters and magnetophoretic chromatography

2015 ◽  
Vol 883 ◽  
pp. 61-66 ◽  
Author(s):  
Donghoon Kwon ◽  
Sanghee Lee ◽  
Myung Mo Ahn ◽  
In Seok Kang ◽  
Ki-Hwan Park ◽  
...  
Keyword(s):  
Magnetic Nanoparticle ◽  
Pathogenic Bacteria ◽  
Colorimetric Detection ◽  
Nanoparticle Clusters

scholarly journals Direct carbon-carbon coupling of furanics with acetic acid over Brønsted zeolites

2016 ◽  
Vol 2 (9) ◽  
pp. e1601072 ◽  
Author(s):  
Abhishek Gumidyala ◽  
Bin Wang ◽  
Steven Crossley
Keyword(s):  
Acetic Acid ◽  
Density Functional ◽  
Reaction Rate ◽  
Activation Barrier ◽  
Density Functional Theory Calculations ◽  
Step Change ◽  
Functional Theory ◽  
Transportation Fuels ◽  
Bond Forming ◽  
Direct Acylation

Effective carbon-carbon coupling of acetic acid to form larger products while minimizing CO2emissions is critical to achieving a step change in efficiency for the production of transportation fuels from sustainable biomass. We report the direct acylation of methylfuran with acetic acid in the presence of water, all of which can be readily produced from biomass. This direct coupling limits unwanted polymerization of furanics while producing acetyl methylfuran. Reaction kinetics and density functional theory calculations illustrate that the calculated apparent barrier for the dehydration of the acid to form surface acyl species is similar to the experimentally measured barrier, implying that this step plays a significant role in determining the net reaction rate. Water inhibits the overall rate, but selectivity to acylated products is not affected. We show that furanic species effectively stabilize the charge of the transition state, therefore lowering the overall activation barrier. These results demonstrate a promising new route to C–C bond–forming reactions for the production of higher-value products from biomass.

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