scholarly journals Identifying suitable tester for evaluating Striga resistant lines using DArTseq markers and agronomic traits

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0253481
Author(s):  
Degife Zebire ◽  
Abebe Menkir ◽  
Victor Adetimirin ◽  
Wende Mengesha ◽  
Silvestro Meseka ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Agronomic Traits ◽  
Genetic Difference ◽  
Inbred Lines ◽  
Agronomic Trait ◽  
Breeding Value ◽  
Hybrid Breeding ◽  
Significant Positive Association ◽  
Maize Inbred Lines ◽  
Resistant Lines

A desirable tester that elicits greater genetic difference in Striga resistance among test crosses in a breeding program has not been reported. Therefore, this study was conducted to characterize 30 Striga resistant yellow endosperm maize inbred lines and three testers with varying resistance levels to Striga using DArTseq SNP markers and agronomic traits to identify a suitable tester for resistance hybrid breeding. Marker-based and agronomic trait-based genetic distances were estimated for yellow endosperm maize inbred lines and testers with varying resistance levels to Striga. The Marker-based cluster analysis separated the Striga resistant lines and testers into two distinct groups. Although the susceptible tester (T3) was the most distantly related to the 30 Striga resistant inbred lines, it exhibited a narrower range in genetic distance estimates and poor agronomic performance under Striga infestation in crosses with the resistant lines. In contrast, the resistant tester (T2) showed a broader range in genetic distance estimates in pairs with the 30 resistant lines. Also, it formed many high yielding hybrids with desirable traits under parasite pressure. Furthermore, the most significant positive association between agronomic trait-based and marker-based distance estimates (r = 0.389, P = 0.01) was observed when T2 has paired with the Striga resistant maize inbred lines. It thus appears that T2 may be used as a suitable tester to determine the breeding value of lines in hybrid maize resistance breeding programs. T2 was the most suitable tester, with a tolerant tester (T1) as an alternative tester to characterize the combining ability of Striga resistant maize inbred lines. This result can also encourage other breeders to investigate testers relative discriminating ability with varying levels of resistance in hybrid breeding for resistance to diseases, pests, and other parasitic plants.

Resistance of some Australian inbred maize lines to maize dwarf mosaic disease

1969 ◽  
Vol 9 (40) ◽  
pp. 541 ◽  
Author(s):  
PW Grogan ◽  
DS Teakle
Keyword(s):  
New South ◽  
Natural Infection ◽  
New South Wales ◽  
Inbred Lines ◽  
Mosaic Disease ◽  
The Other ◽  
Maize Inbred Lines ◽  
South Wales ◽  
Resistant Lines

Seven out of eight maize inbred lines developed at Lawes in Queensland from open-pollinated varieties were resistant to maize dwarf mosaic disease when exposed to natural infection in the field. Five of the seven resistant inbred lines failed to become systemically infected when inoculated with infectious sap in the glasshouse. By contrast, only three out of twenty lines introduced from the U.S.A., and two out of eight lines developed at the Grafton and Glen Innes Breeding Stations in New South Wales, were resistant in the field. All three resistant lines from the U.S.A. were systemically infected when inoculated in the glasshouse, but the two resistant lines from Grafton in New South Wales were not. The resistant Lawes and Grafton maize inbred lines would appear to be better sources of genes conferring resistance to maize dwarf mosaic disease than the other lines tested.

scholarly journals Evaluation of Elite Maize Inbred Lines for Reduced Aspergillus flavus Infection, Aflatoxin Accumulation, and Agronomic Traits

Crop Science ◽  
2019 ◽  
Vol 59 (6) ◽  
pp. 2562-2571 ◽  
Author(s):  
Jacob J. Pekar ◽  
Seth C. Murray ◽  
Thomas S. Isakeit ◽  
Brian T. Scully ◽  
Baozhu Guo ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Agronomic Traits ◽  
Inbred Lines ◽  
Maize Inbred Lines ◽  
Aflatoxin Accumulation

scholarly journals Relationship between hybrid performance and AFLP based genetic distance in highland maize inbred lines

Euphytica ◽  
2007 ◽  
Vol 162 (3) ◽  
pp. 313-323 ◽  
Author(s):  
B. W. Legesse ◽  
A. A. Myburg ◽  
K. V. Pixley ◽  
S. Twumasi-Afriyie ◽  
A. M. Botha
Keyword(s):  
Genetic Distance ◽  
Inbred Lines ◽  
Hybrid Performance ◽  
Maize Inbred Lines ◽  
Highland Maize

scholarly journals Combining Ability Study for Grain Yield and Agronomic Traits of Quality Protein Maize (Zea mays L.) Inbred Lines Adapted to Mid-Altitude Agroecology of Ethiopia

2021 ◽  
Vol 4 (3) ◽  
pp. 286-304
Author(s):  
Lemi Mideksa Yadesa ◽  
Sentayehu Alamerew ◽  
Berhanu Tadesse
Keyword(s):  
Grain Yield ◽  
Combining Ability ◽  
Agronomic Traits ◽  
Inbred Lines ◽  
Quality Protein Maize ◽  
Lattice Design ◽  
Maize Cultivars ◽  
Maize Inbred Lines ◽  
Maize Varieties ◽  
Almost All

In spite of the importance of quality protein maize to alleviate protein deficiency, almost all maize varieties cultivated in Ethiopia are normal maize varieties, which are devoid of lysine and tryptophan. Perusing the combining ability of QPM inbred for grain yield and its components is vital to design appropriate breeding strategies for the development of nutritionally enhanced maize cultivars. A line x tester analysis involving 36 crosses generated by crossing 9  elite maize inbred lines with 4 testers were evaluated for different desirable agronomic traits during the 2019 main season at BNMRC and JARC. The experiment was conducted using alpha lattice design with 3 replications. The objectives were to determine the combining ability of quality protein maize inbred lines, adapted to mid altitude agroecology of Ethiopia for agronomic traits. The crosses were evaluated in alpha lattice design replicated 3 times. Analyses of variances showed significant mean squares due to crosses for almost all the traits studied. GCA mean squares due to lines and testers were significant (P<0.05 or P<0.01) for most studied traits. SCA mean squares were also significant for most attributes across locations. The comparative importance of GCA and SCA variances observed in the current study for most studied traits indicated the preponderance of additive genetic variance in governing these attributes. Only L3 was the best general combiner for grain yield. Inbred line L3, for days to anthesis and L5 for days to silking had negative and significant GCA effects. L5 and L6 displayed negative and significant GCA effects for plant and ear height. Crosses, L2xT4, L3xT4, L4xT4, L5xT2, L6xT3, L7xT2, L9xT1 and L9xT4 were good specific combiners for grain yield. In general, these genotypes help as a source of promising alleles that could be used for forthcoming breeding work in the development of quality protein maize cultivars with desirable traits.

scholarly journals Combining ability and response to CMS in reverse diploid maize lines developed at VIR

2020 ◽  
Vol 2 (4) ◽  
pp. 15-23
Author(s):  
E. B. Khatefov ◽  
B. R. Shomakhov ◽  
R. S. Kushkhova ◽  
R. A. Kudaev ◽  
Z. T. Khashirova ◽  
...  
Keyword(s):  
Combining Ability ◽  
Selection Index ◽  
Inbred Lines ◽  
Breeding Value ◽  
Hybrid Breeding ◽  
Maize Breeding ◽  
Tetraploid Population ◽  
Grain Moisture ◽  
Hybrid Maize ◽  
Period Duration

Abstract. Hybrid maize breeding requires constant renewal of the source material. In this regard, broadening of genetic variation in parental lines is one of the primary tasks in heterotic hybrid breeding programs. The use of reverse diploid inbred lines derived from a tetraploid population is considered as an innovative approach to achieve this goal.Results. The investigated material comprised 106 reverse diploid (rediploid) inbred lines originating from diploid plants selected in segregating selfed progenies of triploid populations and consequently subjected to inbreeding, while triploid populations resulted from a cross between plants of a tetraploid population with a broad genetic basis and a diploid line. The use of a system of crosses with 37 sterile testers belonging to different FAO maize maturity groups allowed the evaluation of the rediploid lines’ combining ability and the response to M and C types of CMS. Field tests were conducted in 2019 in the steppe zone of Kabardino-Balkaria. Forty-six lines (43.3%) with the combining ability ranging from ultra-high to good, and 78 lines (73.6%) maintaining the CMS character were identified. Among them, 59 lines (55.7%) were maintainers for the M type CMS, 15 lines (14.1%)  for C type CMS, and 4 lines maintained sterility for both CMS types. Sixteen lines (15.1%) restored pollen fertility of the forms with M type CMS, 11 lines (10.4%) were restorers for the C-type and one line turned out to be a universal restorer for both CMS types. Ranking by the “sprout - flowering of ears” interstage period duration showed that most of the lines (66.0%) with the ability to maintain sterility or restore male fertility of M and C CMS types, as well as with the combining ability from ultrahigh to good (32.6%) fell into the group with the flowering period duration of 51-55 days. According to the results of the harvested grain moisture assessment, the hybrids ♀(РГС246с × OL213) × ♂92с5986·2·3, ♀714М  ×  ♂1/67-1 and ♀714М  ×  ♂92н136-4, with the values of 13, 6%, 13.9%, 14.0%, respectively, were identified. The hybrids ♀714М × ♂1/67- 1 and ♀(OL563С × KL1392) × ♂92с0653 2 1 2 were characterized by the maximum value of the selection index, i.e. 5.03 and 5.13, respectively.Conclusions. The results of the studies showed the breeding value of rediploid lines as an initial material for hybrid maize breeding. 

scholarly journals Genetic diversity among tropical maize inbred lines as revealed by SSR markers

2020 ◽  
pp. 2010-2019
Author(s):  
Maizura Abu Sin ◽  
Ghizan Saleh ◽  
Nur Ashikin Psyquay Abdullah ◽  
Pedram Kashiani
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Similarity Index ◽  
Inbred Lines ◽  
Hybrid Breeding ◽  
Heterotic Groups ◽  
Breeding Programs ◽  
Maize Inbred Lines

Genetic diversity and phenotypic superiority are important attributes of parental inbred lines for use in hybrid breeding programs. In this study, genetic diversity among 30 maize (Zea mays L.) inbred lines comprising of 28 introductions from the International Maize and Wheat Improvement Center (CIMMYT), one from Indonesia and a locally developed, were evaluated using 100 simple sequence repeat (SSR) markers, as early screening for potential parents of hybrid varieties. All markers were polymorphic, with a total of 550 unique alleles detected on the 100 loci from the 30 inbred lines. Allelic richness ranged from 2 to 13 per locus, with an average of 5.50 alleles (na). Number of effective alleles (ne) was 3.75 per locus, indicating their high effectiveness in revealing diversity among inbred lines. Average polymorphic information content (PIC) was 0.624, with values ranging from 0.178 to 0.874, indicating high informativeness of the markers. High gene diversity was observed on Chromosomes 8 and 4, with high number of effective alleles, indicating their potential usefulness for QTL analysis. The UPGMA dendrogram constructed identified four heterotic groups within a similarity index of 0.350, indicating that these markers were able to group the inbred lines. The three-dimensional PCoA plot also supports the dendrogram grouping, indicating that these two methods complement each other. Inbred lines in different heterotic groups have originated from different backgrounds and population sources. Information on genetic diversity among the maize inbred lines are useful in developing strategies exploiting heterosis in breeding programs

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