scholarly journals How many begomovirus copies are acquired and inoculated by its vector, whitefly (Bemisia tabaci) during feeding?

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258933
Author(s):  
Buddhadeb Roy ◽  
Prosenjit Chakraborty ◽  
Amalendu Ghosh
Keyword(s):  
Bemisia Tabaci ◽  
Absolute Quantification ◽  
New Delhi ◽  
Feeding Duration ◽  
Active Feeding ◽  
Detached Leaves ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Chilli Leaf Curl Virus ◽  
Leaf Curl

Begomoviruses are transmitted by whitefly (Bemisia tabaci Gennadius, Hemiptera: Aleyrodidae) in a persistent-circulative way. Once B. tabaci becomes viruliferous, it remains so throughout its life span. Not much is known about the copies of begomoviruses ingested and/or released by B. tabaci during the process of feeding. The present study reports the absolute quantification of two different begomoviruses viz. tomato leaf curl New Delhi virus (ToLCNDV, bipartite) and chilli leaf curl virus (ChiLCV, monopartite) at different exposure of active acquisition and inoculation feeding using a detached leaf assay. A million copies of both the begomoviruses were acquired by a single B. tabaci with only 5 min of active feeding and virus copy number increased in a logarithmic model with feeding exposure. Whereas, a single B. tabaci could inoculate 8.21E+09 and 4.19E+11 copies of ToLCNDV and ChiLCV, respectively in detached leaves by 5 min of active feeding. Virus copies in inoculated leaves increased with an increase in feeding duration. Comparative dynamics of these two begomoviruses indicated that B. tabaci adult acquired around 14-fold higher copies of ChiLCV than ToLCNDV 24 hrs post feeding. Whereas, the rate of inoculation of ToLCNDV by individual B. tabaci was significantly higher than ChiLCV. The study provides a better understanding of begomovirus acquisition and inoculation dynamics by individual B. tabaci and would facilitate research on virus-vector epidemiology and screening host resistance.

scholarly journals ​Molecular Characterization of Viruses Associated with Leaf Curl Symptoms on Capsicum (Bell Pepper) under Protected Cultivations in Dharwad, Karnataka

2021 ◽  
Author(s):  
C. Channakeshava. M.S. Patil. B. Gurupad ◽  
N.B. Moger
Keyword(s):  
Tomato Leaf ◽  
Bell Pepper ◽  
Ammonium Bromide ◽  
New Delhi ◽  
Tomato Leaf Curl Virus ◽  
Study Results ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Chilli Leaf Curl Virus ◽  
Leaf Curl

Background: Leaf curl or yellowing symptoms, typical of those caused by begomovirus infection, are commonly observed in capsicum (bell pepper) plants in polyhouses. Three capsicum samples with leaf curl and yellowing symptoms were collected from polyhouse at Hi-tech Horticulture, University of Agricultural Sciences, Dharwad, Karnataka, India, during 2017-2018. Methods: Total nucleic acid was isolated from symptomatic and non-symptomatic samples by following CTAB (Cetyl trimethyl ammonium bromide) method and PCR amplified using degenerate and coat protein gene primers of chilli and tomato leaf curl virus. The virus was confirmed by gel electrophoresis with 0.8 per cent agarose. Amplified PCR product was eluted and sent to sequencing (Chromous biotech Pvt. Ltd., Bengaluru). Result: The primer pair could amplify only Chilli leaf curl virus at ~500 bp but not Tomato leaf curl virus. Sequence homology of ChiLCV isolate form Dharwad matched with ChiLCV-Papaya-New Delhi (HM14036), ChiLCV-Chilli-Jodhpu (HM007104) and ChiLCV-Chilli-Noida (HM007114) respectively. The present study results showed that Chilli leaf curl virus is associated with the leaf curl and yellowing symptoms on capsicum under protected cultivation in Dharwad, Karnataka, India.

Identification of Chilli leaf curl virus associated with tomato leaf curl betasatellite infecting Mentha in Oman

2019 ◽  
Vol 41 (2) ◽  
pp. 291-295 ◽  
Author(s):  
Muhammad Shafiq Shahid ◽  
Amir Raza ◽  
Abdullah M. Al-Sadi ◽  
Rob W. Briddon
Keyword(s):  
Tomato Leaf ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Chilli Leaf Curl Virus ◽  
Leaf Curl

scholarly journals First Report of Association of Tomato leaf curl virus-New Delhi with Yellow Mosaic Disease of Luffa cylindrica in India

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1148-1148 ◽  
Author(s):  
S. S. Sohrab ◽  
B. Mandal ◽  
R. P. Pant ◽  
A. Varma
Keyword(s):  
Mosaic Disease ◽  
Tomato Leaf ◽  
New Delhi ◽  
Luffa Cylindrica ◽  
Yellow Mosaic Disease ◽  
Tomato Leaf Curl Virus ◽  
Sponge Gourd ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Sponge gourd (Luffa cylindrica), an important cucurbitaceous vegetable in India, is affected by a disease (2) causing yellow spots on newly emerged leaves, mosaic, mild leaf curling and distortion, small leaves, and misshapen fruits. Nearly 100% of sponge gourd plants were symptomatic in Delhi. Geminivirus-like particles were observed with electron microscopy of uranyl acetate-stained leaf-dip preparations of the diseased plants collected from experimental fields at the Indian Agricultural Research Institute in New Delhi during May and June of 2002. The virus was transmitted by the whitefly (Bemisia tabaci) to sponge and ridge gourd (L. acutangula) after an acquisition and inoculation access period of 24 h each. Whitefly-inoculated plants produced typical yellow mosaic symptoms and contained geminate particles. Nucleic acid extracted from the field-infected and experimentally infected plants hybridized with 32P-labeled probe to DNA-A of Indian cassava mosaic virus, suggesting association of a begomovirus. The viral DNA, isolated by the alkali denaturation method (1) from the experimentally infected sponge gourd plants, was cloned in pBS SK+ at the EcoRI site. A clone with an insert of 2,658 bp was sequenced (GenBank Accession Nos. AJ557219, AJ555488, and AY309957) which shared 89.6 to 95.1% identity with the DNA-A of different strains of Tomato leaf curl virus-New Delhi (ToLCV-NDe). The highest sequence identity (95.1%) was with the severe strain of ToLCV-NDe (GenBank Accession No. U15015). The data suggest that the begomovirus associated with the yellow mosaic disease of L. cylindrica in India is a putative strain of ToLCV-NDe. Reference: (1) K. M. Srivastava et al. J. Virol. Methods 51:297, 1995. (2) A. Varma and B. K. Giri. Virus diseases. Pages 225–245 in: Cucurbits. N. M. Nayar and T. A More, eds. Oxford and IBH Publishing House Private Ltd., New Delhi, India, 1998.

scholarly journals Screening chilli genotypes for whitefly (Bemisia tabaci Genn.) resistance: A vector for chilli leaf curl virus

2020 ◽  
Vol 8 (1) ◽  
pp. 971-979
Author(s):  
Rajeev Kumar Yadav ◽  
PD Kamala Jayanthi ◽  
Manish Kumar ◽  
P Saravan Kumar ◽  
V Keshava Rao ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Leaf Curl Virus ◽  
Chilli Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Tomato leaf curl Karnataka virus from Bangalore, India, Appears to be a Recombinant Begomovirus

2002 ◽  
Vol 92 (6) ◽  
pp. 637-645 ◽  
Author(s):  
Orawan Chatchawankanphanich ◽  
Douglas P. Maxwell
Keyword(s):  
Particle Bombardment ◽  
Phylogenetic Trees ◽  
Tomato Leaf ◽  
New Delhi ◽  
Intergenic Regions ◽  
Biotype B ◽  
Distinct Member ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

The genome of Tomato leaf curl virus (ToLCV) from Bangalore, India, a whitefly-transmitted geminivirus, was cloned (pIND9) and sequenced. The circular DNA of 2,759 nucleotides (U38239) is organized similarly to that of other begomoviruses with monopartite genomes. Comparison of the nucleotide sequence of pIND9 with other tomato-associated begomoviruses from India (Tomato leaf curl Bangalore virus [ToLCBV, Z48182]) and Tomato leaf curl New Delhi virus-Severe (ToLCNdV-Svr, U15015) showed moderate DNA sequence identities (82 to 87%) between capsid protein (CP) genes but low identities (66 to 67%) for the intergenic regions and the replication-associated protein (Rep) genes (75 to 81% identity). Phylogenetic trees generated with nucleotide sequences of the Rep and CP genes of 26 begomoviruses indicated that this ToLCV is distinct from other begomoviruses and that it may be a recombinant virus derived from at least three different viral lineages. Tomatoes (Lycopersicon esculentum) inoculated with the cloned DNA monomer of ToLCV (pIND9) via particle bombardment developed leaf curling and yellowing symptoms. The virus was transmitted by Bemisia tabaci biotype B from tomatoes infected via particle bombardment to healthy tomatoes and by sap inoculation from infected tomatoes to tomato, Nicotiana benthamiana and N. tabacum. This ToLCV is a distinct member of the genus Begomovirus from India that differs from the previously characterized Tomato leaf curl Sadasivanagar virus isolate Bangalore 1 (L12739), ToLCBV (Z48182), ToLCBV isolate Bangalore 4 (AF165098), and the bipartite ToLCNdV (U15015, U15016). Thus, this ToLCV is named Tomato leaf curl Karnataka virus (ToLCKV).

scholarly journals Tomato leaf curl New Delhi virus (Tomato New Delhi virus).

2021 ◽  
Author(s):  
Pedro Gómez ◽  
Miguel Juárez
Keyword(s):  
Bemisia Tabaci ◽  
Mediterranean Basin ◽  
Indian Subcontinent ◽  
Tomato Leaf ◽  
New Delhi ◽  
Dna Virus ◽  
Tomato Leaf Curl ◽  
Whitefly Vector ◽  
The Mediterranean Basin ◽  
Leaf Curl

Abstract Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite, single-stranded DNA virus transmitted by the whitefly, Bemisia tabaci. The virus was first identified in India in 1995 affecting solanaceous crops (Padidam et al., 1995) and thereafter, causing major damage to cucurbit crops on the Indian subcontinent (Zaidi et al., 2017). ToLCNDV was first detected in Europe in 2012, affecting zucchini squash crops in Spain (Juárez et al., 2014), with subsequent detections in Tunisia (Mnari-Hattab et al., 2015), Italy (Panno et al., 2016) and Morocco (Sifres et al., 2018). ToLCNDV is responsible for severe outbreaks of disease in cucurbit crops in the Mediterranean basin (Juárez et al., 2019; Panno et al., 2019) and represents a serious threat to economically important solanaceous crops in the region (Moriones et al., 2017). ToLCNDV appears to be spreading rapidly and is listed as a quarantine pest by EPPO (EPPO, 2019). There are quarantine measures to control its whitefly vector (Bertin et al., 2018).

scholarly journals Identification of Replication Specificity Determinants in Two Strains of Tomato Leaf Curl Virus from New Delhi

1999 ◽  
Vol 73 (7) ◽  
pp. 5481-5489 ◽  
Author(s):  
Anju Chatterji ◽  
Malla Padidam ◽  
Roger N. Beachy ◽  
Claude M. Fauquet
Keyword(s):  
Binding Site ◽  
Tomato Leaf ◽  
Single Amino Acid ◽  
New Delhi ◽  
Rep Protein ◽  
Tomato Leaf Curl Virus ◽  
Severe Strain ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

ABSTRACT We used two strains of tomato leaf curl virus from New Delhi to investigate specificity in replication of their cognate genomes. The strains share 94% sequence identity and are referred to as severe and mild on the basis of symptoms on tomato and tobacco. Replication assays in tobacco protoplasts and plants showed that a single amino acid change, Asn10 to Asp in the N terminus of Rep protein, determines specificity for replication of the two strains based upon its interaction with the origin of replication (ori) sequences. The change of Asp10 to Asn in Rep protein of the mild strain coupled with point mutations at the 3rd and 10th nucleotides of the 13-mer binding site altered its replication ability, resulting in increased levels of virus accumulation. Similarly, changing Asn10 to Asp in Rep protein of the severe strain impaired replication of the virus and altered its severe phenotype in plants. Site-directed mutations made inori and Asn10 of Rep protein suggested that Asn10 recognizes the third base pair of the putative binding site sequence GGTGTCGGAGTC in the severe strain.

Sign in / Sign up

Export Citation Format

Share Document