scholarly journals Profiling of inflammatory cytokines in patients with caustic gastrointestinal tract injury

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260012
Author(s):  
Hao-Tsai Cheng ◽  
Chen-June Seak ◽  
Chien-Cheng Cheng ◽  
Tsung-Hsing Chen ◽  
Chang-Mu Sung ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Inflammatory Cytokines ◽  
Tumor Necrosis ◽  
Necrosis Factor Alpha ◽  
Gastrointestinal Complications ◽  
Severe Group ◽  
Factor Alpha ◽  
Necrosis Factor

Introduction Study of inflammatory cytokines in patients with caustic gastrointestinal tract injury is sketchy. This study investigated the cytokine profiling of patients with caustic substance ingestion, and analyzed the differences between patients with severe and mild injury. Methods This prospective, cross-sectional study enrolled 22 patients admitted to Chang Gung Memorial Hospital between March and October 2018. All patients underwent esophagogastroduodenoscopy in 24 hours. Patients were categorized into two subgroups, as mild (<2b, n = 11) or severe (≥2b, n = 11) group. Results The neutrophil count was higher in severe than mild group (P = 0.032). Patients in mild and severe groups exhibited significantly higher circulating inflammatory cytokines than healthy control, including interleukin (IL)-2, IL-5, IL-8, IL-9, IL-12, IL-13, interferon-gamma inducible protein-10, macrophage inflammatory protein-1 beta, regulated upon activation, normal T cell expressed and presumably secreted and tumor necrosis factor-alpha. Furthermore, the levels of IL-2 and tumor necrosis factor-alpha were significantly higher in patients with severe group than mild group. Although there was no difference in cumulative survival between both groups (P = 0.147), the severe group received more operations (P = 0.035) and suffered more gastrointestinal complications (P = 0.035) than mild group. Conclusion Caustic substance ingestion produces mucosal damages and leads to excessive neutrophils and inflammatory cytokines in peripheral blood.

scholarly journals The concentration of tumor necrosis factor alpha in periapical lesions

2014 ◽  
Vol 61 (1) ◽  
pp. 7-13
Author(s):  
Jelena Popovic ◽  
Tatjana Cvetkovic ◽  
Tanja Dzopalic ◽  
Aleksandar Mitic ◽  
Marija Nikolic ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Inflammatory Cytokines ◽  
Tumor Necrosis ◽  
Clinical Presentation ◽  
Necrosis Factor Alpha ◽  
Periapical Lesions ◽  
Tissue Homogenates ◽  
Factor Alpha ◽  
Necrosis Factor

Introduction. The balance between proinflammatory and anti-inflammatory cytokines plays an important role in the pathogenesis of chronic periapical lesions. The aim of this study was to determine the concentration of TNF-? in tissue homogenates of periapical lesions and analyze its levels in relation to the symptomatology and the size of lesions. Materials and Methods. 93 samples of chronic periapical lesions were obtained after extraction of teeth. Samples were classified according to the clinical presentation as symptomatic and asymptomatic, and according to the size as large and small. The concentration of TNF-? was analyzed using ELISA. Results. The results showed increased production of TNF-? in symptomatic lesions compared to asymptomatic. Higher concentration of TNF-? was demonstrated in large lesions compared to small. Large symptomatic lesions showed greater concentration of TNF-? compared to small symptomatic lesions, while bigger asymptomatic lesions demonstrated higher amount of the cytokines compared to small asymptomatic lesions. Conclusion. Higher concentration of TNF-? in large symptomatic lesions indicates that TNF-? is an important factor responsible for the progression of lesions.

scholarly journals Levels of Intestinal Inflammation and Fibrosis in Resection Specimens after Preoperative Anti-Tumor Necrosis Factor Alpha Treatment in Patients with Crohn’s Disease: A Comparative Pilot Study

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
J. Torle ◽  
P. D. Dabir ◽  
U. Korsgaard ◽  
J. Christiansen ◽  
N. Qvist ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Inflammatory Cytokines ◽  
Tumor Necrosis ◽  
Intestinal Inflammation ◽  
Bowel Resection ◽  
Fibrosis Score ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
Necrosis Factor

Background. Strictures are a common complication in Crohn’s disease (CD), found in more than 50% of patients. They are characterized by the excessive deposition of extracellular proteins in the tissue as a result of the chronic inflammatory process. The effect of anti-tumor necrosis factor alpha (TNF-α) therapy on the development of fibrosis is not yet fully understood. Aim. To investigate whether the degree of intestinal inflammation and fibrosis is correlated with preoperative anti-TNF-α therapy in patients with CD who are undergoing bowel resection. Methods. This unblinded, prospective, single tertiary center, pilot cohort study included all adult patients with CD who underwent elective, laparoscopic, or open intestinal resection. Preoperative investigations included measurement of blood TNF-α concentration, specific antidrug antibodies, and the concentration of selected inflammatory cytokines. Three pathologists independently examined the specimens and assessed the degree of inflammation and fibrosis. Results. Histopathological specimens from 10 patients with CD who underwent ileocecal or ileocolic resections were retrieved. Four of those patients were on anti-TNF-α treatment prior to surgery. The last dose of the anti-TNF-α agent was administered 1–9 weeks prior to bowel resection. Patients on anti-TNF-α treatment had a higher fibrosis score than controls (p=0.01). Anti-TNF-α treatment was not associated with an increase in CD68- or CD163-positive macrophages. There was no significant relationship between the time from the final preoperative anti-TNF-α dose to surgery and the fibrosis score. No significant association was found between the concentration of major inflammatory cytokines, including TNF-α, and the fibrosis score or degree of inflammation. Conclusions. Patients who underwent preoperative anti-TNF-α treatment had a higher fibrosis score than controls.

scholarly journals Tumor Necrosis Factor Alpha and Interleukin 1β Up-Regulate Gastric Mucosal Fas Antigen Expression inHelicobacter pylori Infection

2000 ◽  
Vol 68 (3) ◽  
pp. 1189-1195 ◽  
Author(s):  
JeanMarie Houghton ◽  
Lisa S. Macera-Bloch ◽  
Lawrence Harrison ◽  
Kyung H. Kim ◽  
Reju M. Korah
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Inflammatory Cytokines ◽  
Tumor Necrosis ◽  
Fas Ligand ◽  
Necrosis Factor Alpha ◽  
Fas Antigen ◽  
Factor Alpha ◽  
H Pylori ◽  
Necrosis Factor

ABSTRACT Fas-mediated gastric mucosal apoptosis is gaining attention as a cause of tissue damage due to Helicobacter pyloriinfection. We explored the effects of H. pylori directly, and the effects of the inflammatory environment established subsequent to H. pylori infection, on Fas-mediated apoptosis in a nontransformed gastric mucosal cell line (RGM-1). Exposure to H. pylori-activated peripheral blood mononuclear cells (PBMCs), but not H. pylori itself, induced Fas antigen (Fas Ag) expression, indicating a Fas-regulatory role for inflammatory cytokines in this system. Of various inflammatory cytokines tested, only interleukin 1β and tumor necrosis factor alpha induced Fas Ag expression, and removal of either of these from the conditioned medium abrogated the response. When exposed to Fas ligand, RGM-1 cells treated with PBMC-conditioned medium underwent massive and rapid cell death, interestingly, with a minimal effect on total cell numbers early on. Cell cycle analysis revealed a substantial increase in S phase cells among cells exposed to Fas ligand, suggesting an increase in their proliferative response. Taken together, these data indicate that the immune environment secondary to H. pylori infection plays a critical role in priming gastric mucosal cells to undergo apoptosis or to proliferate based upon their Fas Ag status.

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