Type 1 Fimbriae, a Colonization Factor of Uropathogenic Escherichia coli, Are Controlled by the Metabolic Sensor CRP-cAMP

Adhesion is an influential step for bacterial vigor in clinical micro-environments, type 1 fimbriae are essential virulence factors help uropathogenic E. coli in invasion and colonization of uroepithelial cells, the first step of UTIs and biofilm formation. Type 1 fimbriae of E. coli contain FimH protein at the tip encoding via fimH gene cluster, this study was conducted for determination the fimH gene distribution in uro-pathogenic E. coli isolated from UTIs patients. The results of adhesion assay show that (83.6%) of uropathogenic E. coli were high adherent isolates. While the results of E. coli fimH gene amplification prove that, of all E. coli isolates, the fimH gene was found in (87.1%), while among high adherent isolates it was found in (92.6%), and that Shows the function of type 1 fimbriae in the colonization and infection of urinary tracts in addition to other adhesions virulence agents of uropathogenic E. coli.

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Salicylate increases the expression of marA and reduces in vitro biofilm formation in uropathogenic Escherichia coli by decreasing type 1 fimbriae expression

Virulence ◽

10.4161/viru.19205 ◽

2012 ◽

Vol 3 (3) ◽

pp. 280-285 ◽

Cited By ~ 21

Author(s):

Jordi Vila ◽

Sara M. Soto

Keyword(s):

Escherichia Coli ◽

Biofilm Formation ◽

Uropathogenic Escherichia Coli ◽

Type 1 Fimbriae

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Type 1 fimbriae and extracellular polysaccharides are preeminent uropathogenic Escherichia coli virulence determinants in the murine urinary tract

Molecular Microbiology ◽

10.1046/j.1365-2958.2002.03078.x ◽

2002 ◽

Vol 45 (4) ◽

pp. 1079-1093 ◽

Cited By ~ 173

Author(s):

Farah K. Bahrani-Mougeot ◽

Eric L. Buckles ◽

C. V. Lockatell ◽

J. R. Hebel ◽

D. E. Johnson ◽

...

Keyword(s):

Escherichia Coli ◽

Urinary Tract ◽

Uropathogenic Escherichia Coli ◽

Extracellular Polysaccharides ◽

Virulence Determinants ◽

Type 1 Fimbriae

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Faculty Opinions recommendation of Adaptive mutations in the signal peptide of the type 1 fimbrial adhesin of uropathogenic Escherichia coli.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1119456.575652 ◽

2008 ◽

Author(s):

Esther Bullitt

Keyword(s):

Escherichia Coli ◽

Signal Peptide ◽

Uropathogenic Escherichia Coli ◽

Adaptive Mutations ◽

Fimbrial Adhesin

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Role of the ceramide-signaling pathway in cytokine responses to P-fimbriated Escherichia coli.

Journal of Experimental Medicine ◽

10.1084/jem.183.3.1037 ◽

1996 ◽

Vol 183 (3) ◽

pp. 1037-1044 ◽

Cited By ~ 115

Author(s):

M Hedlund ◽

M Svensson ◽

A Nilsson ◽

R D Duan ◽

C Svanborg

Keyword(s):

Escherichia Coli ◽

Signaling Pathway ◽

Kinase Inhibitors ◽

Human Kidney ◽

Cytokine Response ◽

Protein Kinase Inhibitors ◽

E Coli ◽

Type 1 Fimbriae ◽

Outer Leaflet

Escherichia coli express fimbriae-associated adhesins through which they attach to mucosal cells and activate a cytokine response. The receptors for E. coli P fimbriae are the globoseries of glycosphingolipids; Gal alpha 1-->4Gal beta-containing oligosaccharides bound to ceramide in the outer leaflet of the lipid bilayer. The receptors for type 1 fimbriae are mannosylated glycoproteins rather than glycolipids. This study tested the hypothesis that P-fimbriated E. coli elicit a cytokine response through the release of ceramide in the receptor-bearing cell. We used the A498 human kidney cell line, which expressed functional receptors for P and type 1 fimbriae and secreted higher levels of interleukin (IL)-6 when exposed to the fimbriated strains than to isogenic nonfimbriated controls. P-fimbriated E. coli caused the release of ceramide and increased the phosphorylation of ceramide to ceramide 1-phosphate. The IL-6 response to P-fimbriated E. coli was reduced by inhibitors of serine/threonine kinases but not by other protein kinase inhibitors. In contrast, ceramide levels were not influenced by type 1-fimbriated E. coli, and the IL-6 response was insensitive to the serine/threonine kinase inhibitors. These results demonstrate that the ceramide-signaling pathway is activated by P-fimbriated E. coli, and that the receptor specificity of the P fimbriae influences this process. We propose that this activation pathway contributes to the cytokine induction by P-fimbriated E. coli in epithelial cells.

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