scholarly journals HYR1-Mediated Detoxification of Reactive Oxygen Species Is Required for Full Virulence in the Rice Blast Fungus

2011 ◽  
Vol 7 (4) ◽  
pp. e1001335 ◽  
Author(s):  
Kun Huang ◽  
Kirk J. Czymmek ◽  
Jeffrey L. Caplan ◽  
James A. Sweigard ◽  
Nicole M. Donofrio
Keyword(s):  
Reactive Oxygen Species ◽  
Rice Blast ◽  
Reactive Oxygen ◽  
Rice Blast Fungus ◽  
Oxygen Species ◽  
Blast Fungus

scholarly journals Suppression of plant-generated reactive oxygen species is required for successful infection by the rice blast fungus

Virulence ◽  
2011 ◽  
Vol 2 (6) ◽  
pp. 559-562 ◽  
Author(s):  
Kun Huang ◽  
Kirk J. Czymmek ◽  
Jeffrey L. Caplan ◽  
James A. Sweigard ◽  
Nicole M. Donofrio
Keyword(s):  
Reactive Oxygen Species ◽  
Rice Blast ◽  
Reactive Oxygen ◽  
Rice Blast Fungus ◽  
Oxygen Species ◽  
Blast Fungus ◽  
Successful Infection

Release of elicitors from rice blast spores under the action of reactive oxygen species

2010 ◽  
Vol 57 (5) ◽  
pp. 615-619 ◽  
Author(s):  
T. S. Zakharenkova ◽  
A. A. Aver’yanov ◽  
T. D. Pasechnik ◽  
V. P. Lapikova ◽  
C. J. Baker
Keyword(s):  
Reactive Oxygen Species ◽  
Rice Blast ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Generation of reactive oxygen species by fungal NADPH oxidases is required for rice blast disease

2007 ◽  
Vol 104 (28) ◽  
pp. 11772-11777 ◽  
Author(s):  
M. J. Egan ◽  
Z.-Y. Wang ◽  
M. A. Jones ◽  
N. Smirnoff ◽  
N. J. Talbot
Keyword(s):  
Reactive Oxygen Species ◽  
Rice Blast ◽  
Reactive Oxygen ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Oxygen Species ◽  
Nadph Oxidases

scholarly journals Every Coin Has Two Sides: Reactive Oxygen Species during Rice–Magnaporthe oryzae Interaction

2019 ◽  
Vol 20 (5) ◽  
pp. 1191 ◽  
Author(s):  
Yanjun Kou ◽  
Jiehua Qiu ◽  
Zeng Tao
Keyword(s):  
Reactive Oxygen Species ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Blast Resistance ◽  
Reactive Oxygen ◽  
Redox Balance ◽  
Ros Generation ◽  
Oxygen Species ◽  
Rice Blast Resistance ◽  
Ros Accumulation

Reactive oxygen species (ROS) are involved in many important processes, including the growth, development, and responses to the environments, in rice (Oryza sativa) and Magnaporthe oryzae. Although ROS are known to be critical components in rice–M. oryzae interactions, their regulations and pathways have not yet been completely revealed. Recent studies have provided fascinating insights into the intricate physiological redox balance in rice–M. oryzae interactions. In M. oryzae, ROS accumulation is required for the appressorium formation and penetration. However, once inside the rice cells, M. oryzae must scavenge the host-derived ROS to spread invasive hyphae. On the other side, ROS play key roles in rice against M. oryzae. It has been known that, upon perception of M. oryzae, rice plants modulate their activities of ROS generating and scavenging enzymes, mainly on NADPH oxidase OsRbohB, by different signaling pathways to accumulate ROS against rice blast. By contrast, the M. oryzae virulent strains are capable of suppressing ROS accumulation and attenuating rice blast resistance by the secretion of effectors, such as AvrPii and AvrPiz-t. These results suggest that ROS generation and scavenging of ROS are tightly controlled by different pathways in both M. oryzae and rice during rice blast. In this review, the most recent advances in the understanding of the regulatory mechanisms of ROS accumulation and signaling during rice–M. oryzae interaction are summarized.

scholarly journals Identifying unique and overlapping roles of reactive oxygen species in rice blast and Southern corn leaf blight

2017 ◽  
Author(s):  
Benjamin Horwitz ◽  
Nicole M. Donofrio
Keyword(s):  
Reactive Oxygen Species ◽  
Rice Blast ◽  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Reactive Oxygen ◽  
Leaf Blight ◽  
Confocal Imaging ◽  
Oxygen Species ◽  
Fungal Genes ◽  
Corn Leaf Blight

Plants and their fungal pathogens both produce reactive oxygen species (ROS). CytotoxicROS act both as stressors and signals in the plant-fungal interaction. In biotrophs, a compatible interaction generates little ROS, but is followed by disease. An incompatible interaction results in a strong oxidative burst by the host, limiting infection. Necrotrophs, in contrast, thrive on dead and dying cells in an oxidant-rich local environment. Rice blast, Magnaportheoryzae, a hemibiotroph, occurs worldwide on rice and related hosts and can decimate enough rice each year to feed sixty million people. Cochliobolusheterostrophus, a necrotroph, causes Southern corn leaf blight (SLB), responsible for a major epidemic in the 1970s. The objectives of our study of ROS signaling and response in these two cereal pathogens were:  Confocal imaging of ROS production using genetically encoded redox sensor in two pathosystems over time. Forward genetic screening of HyPer sensor lines in two pathosystems for fungal genes involved in altered ROSphenotypes. RNA-seq for discovery of genes involved in ROS-related stress and signaling in two pathosystems. Revisions to the research plan: Library construction in SLB was limited by low transformation efficiency, compounded by a protoplasting enzyme being unavailable during most of year 3. Thus Objective 2 for SLB re-focused to construction of sensor lines carrying deletion mutations in known or candidate genes involved in ROS response. Imaging on rice proved extremely challenging, so mutant screening and imaging were done with a barley-infecting line, already from the first year.   In this project, ROS imaging at unprecedented time and spatial resolution was achieved, using genetically-encoded ratio sensors in both pathogens. This technology is currently in use for a large library of rice blast mutants in the ROS sensor background, and Southern corn leaf blight mutants in final stages of construction. The imaging methods developed here to follow the redox state of plant pathogens in the host tissue should be applicable to fungal pathogens in general. Upon completion of mutant construction for SCLB we hope to achieve our goal of comparison between intracellular ROS status and response in hemibiotroph and necrotroph cereal pathogens. 

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