scholarly journals Genome-wide discovery of novel M1T1 group A streptococcal determinants important for fitness and virulence during soft-tissue infection

2017 ◽  
Vol 13 (8) ◽  
pp. e1006584 ◽  
Author(s):  
Yoann Le Breton ◽  
Ashton T. Belew ◽  
Jeffrey A. Freiberg ◽  
Ganesh S. Sundar ◽  
Emrul Islam ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Soft Tissue Infection ◽  
Tissue Infection ◽  
Genome Wide ◽  
Group A

scholarly journals Phosphotransferase System Uptake and Metabolism of the β-Glucoside Salicin Impact Group A Streptococcal Bloodstream Survival and Soft Tissue Infection

2020 ◽  
Vol 88 (10) ◽  
Author(s):  
Rezia Era Braza ◽  
Aliyah B. Silver ◽  
Ganesh S. Sundar ◽  
Sarah E. Davis ◽  
Afrooz Razi ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Soft Tissue Infection ◽  
Tissue Infection ◽  
Phosphotransferase System ◽  
Content Type ◽  
Growth And Survival ◽  
Successful Infection ◽  
Group A ◽  
The Individual

ABSTRACT Streptococcus pyogenes (group A Streptococcus [GAS]), a major human-specific pathogen, relies on efficient nutrient acquisition for successful infection within its host. The phosphotransferase system (PTS) couples the import of carbohydrates with their phosphorylation prior to metabolism and has been linked to GAS pathogenesis. In a screen of an insertional mutant library of all 14 annotated PTS permease (EIIC) genes in MGAS5005, the annotated β-glucoside PTS transporter (bglP) was found to be crucial for GAS growth and survival in human blood and was validated in another M1T1 GAS strain, 5448. In 5448, bglP was shown to be in an operon with a putative phospho-β-glucosidase (bglB) downstream and a predicted antiterminator (licT) upstream. Using defined nonpolar mutants of the β-glucoside permease (bglP) and β-glucosidase enzyme (bglB) in 5448, we showed that bglB, not bglP, was important for growth in blood. Furthermore, transcription of the licT-blgPB operon was found to be repressed by glucose and induced by the β-glucoside salicin as the sole carbon source. Investigation of the individual bglP and bglB mutants determined that they influence in vitro growth in the β-glucoside salicin; however, only bglP was necessary for growth in other non-β-glucoside PTS sugars, such as fructose and mannose. Additionally, loss of BglP and BglB suggests that they are important for the regulation of virulence-related genes that control biofilm formation, streptolysin S (SLS)-mediated hemolysis, and localized ulcerative lesion progression during subcutaneous infections in mice. Thus, our results indicate that the β-glucoside PTS transports salicin and its metabolism can differentially influence GAS pathophysiology during soft tissue infection.

Steroids in the Treatment of Group A Streptococcal Necrotizing Soft Tissue Infection

2011 ◽  
Vol 12 (1) ◽  
pp. 77-81 ◽  
Author(s):  
Addison K. May ◽  
Titus L. Daniels ◽  
William T. Obremskey ◽  
Allen B. Kaiser ◽  
Thomas R. Talbot
Keyword(s):  
Soft Tissue ◽  
Soft Tissue Infection ◽  
Tissue Infection ◽  
Necrotizing Soft Tissue Infection ◽  
Group A

scholarly journals Necrotising soft tissue infection in a UK metropolitan population

2015 ◽  
Vol 97 (1) ◽  
pp. 46-51 ◽  
Author(s):  
GE Glass ◽  
F Sheil ◽  
JC Ruston ◽  
PEM Butler
Keyword(s):  
Soft Tissue ◽  
Soft Tissue Infection ◽  
Young Patients ◽  
Retrospective Chart Review ◽  
Tissue Infection ◽  
Teaching Hospitals ◽  
Risk Indicator ◽  
First Line ◽  
Group A ◽  
Lrinec Score

Introduction Necrotising soft tissue infection (NSTI) is a rare but life threatening diagnosis. Geographic, economic and social variances influence presentation and prognosis. As the current literature does not reflect a UK metropolitan population, we conducted a retrospective chart review to establish pertinent features relevant to our practice. Methods Patients with histologically confirmed diagnoses of NSTI presenting to two London teaching hospitals between January 2007 and July 2013 were included in the study. Features of presentation, surgical and medical management, microbiological findings and outcome were evaluated. Results Twenty-four patients with histologically confirmed NSTI were included. Two age clusters were identified, with means of 46 years (standard deviation [SD]: 10 years) and 80 years (SD: 6 years). Pain, erythema and sepsis were common findings. Hypertension, hypercholesterolaemia and type II diabetes mellitus were common co-morbidities. A third of younger patients had human immunodeficiency virus or hepatitis C, with a quarter dependent on drugs and/or alcohol. The mean Laboratory Risk Indicator for Necrotising Fasciitis (LRINEC) score was 5.8 (SD: 3.3). The lower extremities, groin and perineum were common sites of infection. Fourteen patients required inotropic support and seventeen required transfusions. The median number of surgical procedures was 5 (range: 1–17). Group A Streptococcus was the most frequently identified pathogen. Five patients died. Being elderly, female sex and failure to use clindamycin as a first-line antibiotic were associated with significantly higher mortality. Conclusions In contrast to other recent series, group A streptococcal monomicrobial NSTI remains the most common presentation in our population. Survival is anticipated in young patients, regardless of premorbid status. Elderly patients have a poor prognosis. The negative predictive value of the LRINEC score is questioned. Use of clindamycin as a first-line antibiotic is supported.

scholarly journals A Rare Cause of Necrotizing Fasciitis: Psoas Abscess

2005 ◽  
Vol 12 (4) ◽  
pp. 242-245
Author(s):  
AYC Siu ◽  
SL Kwok ◽  
CH Chung ◽  
KK Lai
Keyword(s):  
Soft Tissue ◽  
Necrotizing Fasciitis ◽  
Soft Tissue Infection ◽  
Psoas Abscess ◽  
Fatal Case ◽  
Tissue Infection ◽  
Mortality And Morbidity ◽  
Group A ◽  
Clinical Tools ◽  
Very High

Necrotizing fasciitis always carries very high mortality and morbidity rates. It can be due to group A beta-haemolytic streptococci, which are traditionally described as the flesh-eating bacteria. More commonly, it is related to a mixed growth of bacteria that can be secondary to trauma or surgery. Secondary necrotizing fasciitis due to concomitant soft tissue infection is uncommon. We reported a fatal case of necrotizing fasciitis which was caused by a coexisting psoas abscess. A search for concomitant soft tissue infection is warranted in patients presenting with necrotizing fasciitis. This article also reviewed the clinical tools that may help to make an early diagnosis of the disease.

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