scholarly journals Mass Spectrometric Identification of Cardiac Troponin T in Urine of Patients Suffering from Acute Myocardial Infarction

2017 ◽  
Vol 2 (6) ◽  
pp. 857-867 ◽  
Author(s):  
Alexander S. Streng ◽  
Noreen van der Linden ◽  
Jordy M.M. Kocken ◽  
Otto Bekers ◽  
Freek G. Bouwman ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cardiac Troponin ◽  
Troponin T ◽  
Mass Spectrometric ◽  
Cardiac Troponin T ◽  
Spectrometric Identification ◽  
Mass Spectrometric Identification

scholarly journals 1-h Evaluation for Acute Myocardial Infarction Using the Generation 5 Cardiac Troponin T Assay

2018 ◽  
Vol 72 (21) ◽  
pp. 2677-2679 ◽  
Author(s):  
Richard M. Nowak ◽  
Chaun M. Gandolfo ◽  
Gordon Jacobsen ◽  
Robert H. Christenson ◽  
Michele Moyer ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cardiac Troponin ◽  
Troponin T ◽  
Cardiac Troponin T

scholarly journals Prospective validation of a 1-hour algorithm to rule-out and rule-in acute myocardial infarction using a high-sensitivity cardiac troponin T assay

2015 ◽  
Vol 187 (8) ◽  
pp. E243-E252 ◽  
Author(s):  
Tobias Reichlin ◽  
Raphael Twerenbold ◽  
Karin Wildi ◽  
Maria Rubini Gimenez ◽  
Nathalie Bergsma ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cardiac Troponin ◽  
Troponin T ◽  
High Sensitivity ◽  
Cardiac Troponin T ◽  
Rule Out

Elevated admission cardiac troponin T is associated with microvascular dysfunction in acute myocardial infarction treated with emergency angioplasty

2009 ◽  
Vol 10 (8) ◽  
pp. 664-668 ◽  
Author(s):  
Italo Porto ◽  
Francesco Burzotta ◽  
Marta Brancati ◽  
Carlo Trani ◽  
Antonella Lombardo ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cardiac Troponin ◽  
Troponin T ◽  
Microvascular Dysfunction ◽  
Cardiac Troponin T

scholarly journals Identification and Characterization of Cardiac Troponin T Fragments in Serum of Patients Suffering from Acute Myocardial Infarction

2017 ◽  
Vol 63 (2) ◽  
pp. 563-572 ◽  
Author(s):  
Alexander S Streng ◽  
Douwe de Boer ◽  
William P T M van Doorn ◽  
Freek G Bouwman ◽  
Edwin C M Mariman ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Mass Spectrometry ◽  
Acute Myocardial Infarction ◽  
Western Blotting ◽  
Cardiac Troponin ◽  
Epitope Mapping ◽  
Troponin T ◽  
Amino Acid Sequences ◽  
Cardiac Troponin T ◽  
Selected Ion Monitoring

AbstractBACKGROUNDCardiac troponin T (cTnT) is the preferred biomarker for the diagnosis of acute myocardial infarction (AMI). It has been suggested that cTnT is present predominantly in fragmented forms in human serum following AMI. In this study, we have used a targeted mass spectrometry assay and epitope mapping using Western blotting to confirm this hypothesis.METHODScTnT was captured from the serum of 12 patients diagnosed with AMI using an immunoprecipitation technique employing the M11.7 catcher antibody and fractionated with SDS-PAGE. Coomassie-stained bands of 4 patients at 37, 29, and 16 kDa were excised from the gel, digested with trypsin, and analyzed on a Q Exactive instrument set on targeted Selected Ion Monitoring mode with data-dependent tandem mass spectrometry (MS/MS) for identification. Western blotting employing 3 different antibodies was used for epitope mapping.RESULTSTen cTnT peptides of interest were targeted. By using MS/MS, all of these peptides were identified in the 37-kDa, intact, cTnT band. In the 29- and 16-kDa fragment bands, 8 and 4 cTnT-specific peptides were identified, respectively. Some of these peptides were “semitryptic,” meaning that their C-termini were not formed by trypsin cleavage. The C-termini of these semitryptic peptides represent the C-terminal end of the cTnT molecules present in these bands. These results were confirmed independently by epitope mapping.CONCLUSIONSUsing LC-MS, we have succeeded in positively identifying the 29- and 16-kDa fragment bands as cTnT-derived products. The amino acid sequences of the 29- and 16-kDa fragments are Ser79-Trp297 and Ser79-Gln199, respectively.

Plasma cardiac troponin T closely correlates with infarct size in a mouse model of acute myocardial infarction

2002 ◽  
Vol 325 (1-2) ◽  
pp. 87-90 ◽  
Author(s):  
Bernhard Metzler ◽  
Angelika Hammerer-Lercher ◽  
Johannes Jehle ◽  
Hermann Dietrich ◽  
Otmar Pachinger ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Mouse Model ◽  
Infarct Size ◽  
Cardiac Troponin ◽  
Troponin T ◽  
Cardiac Troponin T

Cardiac Troponin T as a Marker in the Diagnosis of Acute Myocardial Infarction at Autopsy

2017 ◽  
Vol 17 (1) ◽  
pp. 88
Author(s):  
B Venkata Naga Mohan Rao ◽  
Bandi Sree Ramulu ◽  
B Raju
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cardiac Troponin ◽  
Troponin T ◽  
Cardiac Troponin T
Sign in / Sign up

Export Citation Format

Share Document