Evaluation of Polymerase Chain Reaction, Adenosine Deaminase, and Interferon-γ in Pleural Fluid for the Differential Diagnosis of Pleural Tuberculosis

CHEST Journal ◽  
2000 ◽  
Vol 118 (5) ◽  
pp. 1355-1364 ◽  
Author(s):  
Maria Virginia Villegas ◽  
Luz Angela Labrada ◽  
Nancy Gore Saravia
Keyword(s):  
Polymerase Chain Reaction ◽  
Differential Diagnosis ◽  
Adenosine Deaminase ◽  
Pleural Fluid ◽  
Interferon Γ ◽  
Chain Reaction ◽  
Pleural Tuberculosis ◽  
Polymerase Chain

scholarly journals MOLECULAR DIAGNOSIS OF CLINICALLY SUSPECTED PLEURAL TUBERCULOSIS IN MOROCCO

2019 ◽  
Author(s):  
I. CHAOUI ◽  
S. Taoudi ◽  
A. Oudghiri ◽  
J. Benamor ◽  
J. Bourkadi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Prospective Study ◽  
Pleural Fluid ◽  
Sensitivity And Specificity ◽  
Molecular Diagnosis ◽  
Histopathologic Examination ◽  
Chain Reaction ◽  
Pleural Tuberculosis ◽  
Polymerase Chain ◽  
Pleural Tissue

Pleural tuberculosis (pTB) is a very common form of extrapulmonary tuberculosis (TB). Its diagnosis presents worldwidea major burning challenge due to the limitations of available conventional diagnosis tools. These latter include microscopic examination of the pleural fluid for acid-fast bacilli, mycobacterial culture of pleural fluid in solid or liquid media, sputum or pleural tissue, and histopathological examination of pleural tissue; these tests have recognized limitations for clinical use. Hence, to overcome these limitations, attention has been devoted to new nucleic acid amplification (NAA) diagnosistests such as the polymerase chain reaction (PCR) and real-time PCR (RT-PCR), owing to their accuracy, rapidity, high sensitivity and specificity. Within this context, this prospective study was conducted to evaluate the performance of molecular diagnosis methods for differentiation between tuberculosis and non-tuberculosis pleural effusions. Fifty patients with pleural effusion were enrolled in this prospective study in Rabat, Morocco. The efficacy of conventional polymerase chain reaction (PCR) in the diagnosis of tuberculous pleurisy by targeting IS6110 and mycobacterial internal transcribed spacer(MYITS) was evaluated against histopathologic examination and culture results.Our results showed that IS6110 PCR could “rule in” pTB, the sensitivity and specificity being 41.6 % and 85.7 % respectively.Therefore, the findings confirmed that molecular tests have a relatively high specificity in EPTB but lower sensitivity, thus a positive test is treated as a pTB case whereas negative one cannot exclude the disease. Although the study was limited by small sample size, it adds to the body of evidence of usefulness of molecular testing as adjunctsto histopathologic examination for accurate diagnosis of pTB, to treat timely and to avoid the emergence and spread of drug resistant pTB. However, further efforts should be made to increase the sensitivity of NAA methods and to identify the best molecular targets to be useful in clinical practice.

Polymerase chain reaction in the diagnosis of parotid gland tuberculosis

1998 ◽  
Vol 112 (5) ◽  
pp. 494-496 ◽  
Author(s):  
Enis Alpin Güneri ◽  
Ahmet Ömer İkiz ◽  
Nese Atabey ◽  
Özlem İzci ◽  
Semih Sütay
Keyword(s):  
Polymerase Chain Reaction ◽  
Differential Diagnosis ◽  
Parotid Gland ◽  
Mycobacterial Infection ◽  
Social Characteristics ◽  
Causative Organism ◽  
Chain Reaction ◽  
First Case ◽  
Polymerase Chain ◽  
High Degree

AbstractA parotid gland mass with presenting features of malignancy is a diagnostic and therapeutic challenge. The histological nature of the lesion must be clearly determined before proceeding with facial nerve sacrificing surgery. Although rare, tuberculosis of the parotid gland must be included in the differential diagnosis of a parotid gland mass especially when the social characteristics of the patient suggests a mycobacterial infection. Primary tuberculosis of the parotid gland is generally encountered among populations with a high incidence of pulmonary disease. The difficulty in the differential diagnosis of a parotid gland malignancy may be helped by a high degree of clinical suspicion, since laboratory tests generally do not identify the specific causative organism. This article reports the first case of parotid gland tuberculosis with clinical and radiodiagnostical features simulating malignancy in which the diagnosis was confirmed by the polymerase chain reaction (PCR).

scholarly journals The utility of real-time polymerase chain reaction in detecting Coccidioides immitis among clinical specimens in the Central California San Joaquin Valley

2018 ◽  
Vol 57 (6) ◽  
pp. 688-693 ◽  
Author(s):  
Dominic Dizon ◽  
Marilyn Mitchell ◽  
Bernadette Dizon ◽  
Robert Libke ◽  
Michael W Peterson
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Pleural Fluid ◽  
Clinical Setting ◽  
Predictive Value ◽  
Coccidioides Immitis ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Acute Pneumonia ◽  
Polymerase Chain

AbstractCoccidioidomycosis, the fungal infection caused by dimorphic Coccidioides species, is typically diagnosed by histopathologic identification of spherules, by culture, or by serology. These tests are reliable but time-intensive, delaying diagnosis and treatment. Rapid real-time polymerase chain reaction (RT-PCR) can be performed and was validated to identify Coccidioides immitis using an in-house developed assay for the Becton Dickinson molecular instrument (BD MAXTM). These studies were performed using patient samples that had been shown to be positive on previously set up fungal cultures. To evaluate this new RT-PCR test in the clinical setting, we conducted a retrospective chart review of patients (N = 1160) who underwent Coccidioides PCR (Cocci PCR) on clinical samples between March 1, 2014, and Dec 31, 2016. We abstracted clinical, microbiologic, serologic, radiographic, treatment, and follow-up data. Specimens of cerebrospinal fluid (CSF), bronchioalveolar lavage fluid (BAL), lung tissue biopsy (LTB), sputum, and pleural fluid were evaluated to determine sensitivity and specificity. Of the 113 specimens that tested positive for Cocci PCR, all had clinical disease defined by traditional clinical criteria, yielding 100% specificity. Overall sensitivity was 74% versus 46% for fungal culture and was available in 4 hours rather than 1–2 weeks. Sensitivities varied by source material and clinical setting. CSF had a sensitivity of 59%, BAL for acute pneumonia 91%, sputum for acute pneumonia 94%, pleural fluid 86%, but LTB for lung nodules only 44%. Overall positive predictive value (PPV) was 100%, while negative predictive value (NPV) was 96%, but again this varied by specimen and clinical setting. Our experience with clinical testing of >1160 specimens over 2–3 years shows we can utilize this technology to improve our ability to diagnose disease but that the sensitivity varies by specimen source and clinical setting.

scholarly journals Evaluation of Nested Polymerase Chain Reaction for Detecting Myocobacterial DNA in Pleural Fluid

1995 ◽  
Vol 69 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Kazuyoshi KUWANO ◽  
Wakio MINAMIDE ◽  
Shinji KUSUNOKI ◽  
Hirotsune IGIMI ◽  
Tetsuro FUJIKI ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pleural Fluid ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain
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