Fibered Confocal Fluorescence Microscopy Imaging in Diffuse Parenchymal Lung Diseases

CHEST Journal ◽  
2015 ◽  
Vol 148 (4) ◽  
pp. 788A
Author(s):  
Peng Meng ◽  
Gan Liang Tan ◽  
Su Ying Low ◽  
Angela Takano ◽  
Yuen Li Ng ◽  
...  
2007 ◽  
Vol 6 (2) ◽  
pp. 7290.2007.00004 ◽  
Author(s):  
Marie-Sophie Martina ◽  
Jean-Paul Fortin ◽  
Laure Fournier ◽  
Christine Ménager ◽  
Florence Gazeau ◽  
...  

2021 ◽  
Vol 26 (09) ◽  
Author(s):  
Brendon S. Restall ◽  
Pradyumna Kedarisetti ◽  
Nathaniel J. M. Haven ◽  
Matthew T. Martell ◽  
Roger J. Zemp

2004 ◽  
Vol 41 (5) ◽  
pp. 400-411 ◽  
Author(s):  
Elisabeth Laemmel ◽  
Magalie Genet ◽  
Georges Le Goualher ◽  
Aymeric Perchant ◽  
Jean-François Le Gargasson ◽  
...  

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Biliana Todorova ◽  
Nina Salabert ◽  
Sabine Tricot ◽  
Raphaël Boisgard ◽  
Mélanie Rathaux ◽  
...  

Purpose. We developed a new approach to visualize skin Langerhans cells by in vivo fluorescence imaging in nonhuman primates. Procedures. Macaques were intradermally injected with a monoclonal, fluorescently labeled antibody against HLA-DR molecule and were imaged for up to 5 days by fibered confocal microscopy (FCFM). Results. The network of skin Langerhans cells was visualized by in vivo fibered confocal fluorescence microscopy. Quantification of Langerhans cells revealed no changes to cell density with time. Ex vivo experiments confirmed that injected fluorescent HLA-DR antibody specifically targeted Langerhans cells in the epidermis. Conclusions. This study demonstrates the feasibility of single-cell, in vivo imaging as a noninvasive technique to track Langerhans cells in nontransgenic animals.


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