In Vivo Imaging of the Bronchial Wall Microstructure Using Fibered Confocal Fluorescence Microscopy

2009 ◽  
Vol 2009 ◽  
pp. 167-169
Author(s):  
L.T. Tanoue
Keyword(s):  
Fluorescence Microscopy ◽  
In Vivo Imaging ◽  
Confocal Fluorescence Microscopy ◽  
Bronchial Wall ◽  
Confocal Fluorescence ◽  
Fibered Confocal Fluorescence Microscopy

scholarly journals Magnetic Targeting of Rhodamine-Labeled Superparamagnetic Liposomes to Solid Tumors: In Vivo Tracking by Fibered Confocal Fluorescence Microscopy

2007 ◽  
Vol 6 (2) ◽  
pp. 7290.2007.00004 ◽  
Author(s):  
Marie-Sophie Martina ◽  
Jean-Paul Fortin ◽  
Laure Fournier ◽  
Christine Ménager ◽  
Florence Gazeau ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Solid Tumors ◽  
Confocal Fluorescence Microscopy ◽  
Magnetic Targeting ◽  
Confocal Fluorescence ◽  
Fibered Confocal Fluorescence Microscopy

scholarly journals Fibered Confocal Fluorescence Microscopy for the Noninvasive Imaging of Langerhans Cells in Macaques

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Biliana Todorova ◽  
Nina Salabert ◽  
Sabine Tricot ◽  
Raphaël Boisgard ◽  
Mélanie Rathaux ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Langerhans Cells ◽  
Ex Vivo ◽  
Confocal Fluorescence Microscopy ◽  
Noninvasive Technique ◽  
Hla Dr ◽  
Confocal Fluorescence ◽  
In Vivo Fluorescence Imaging ◽  
Fibered Confocal Fluorescence Microscopy

Purpose. We developed a new approach to visualize skin Langerhans cells by in vivo fluorescence imaging in nonhuman primates. Procedures. Macaques were intradermally injected with a monoclonal, fluorescently labeled antibody against HLA-DR molecule and were imaged for up to 5 days by fibered confocal microscopy (FCFM). Results. The network of skin Langerhans cells was visualized by in vivo fibered confocal fluorescence microscopy. Quantification of Langerhans cells revealed no changes to cell density with time. Ex vivo experiments confirmed that injected fluorescent HLA-DR antibody specifically targeted Langerhans cells in the epidermis. Conclusions. This study demonstrates the feasibility of single-cell, in vivo imaging as a noninvasive technique to track Langerhans cells in nontransgenic animals.

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