Serum or Plasma Sample Preparation for Two-Dimensional Gel Electrophoresis

2005 ◽  
pp. 49-54 ◽  
Author(s):  
Anthony G. Sullivan ◽  
Stephen Russell ◽  
Henry Brzeski ◽  
Richard I. Somiari ◽  
Craig D. Shriver
Keyword(s):  
Sample Preparation ◽  
Gel Electrophoresis ◽  
Plasma Sample ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis of peripheral nerve proteins: Optimized sample preparation

2007 ◽  
Vol 159 (1) ◽  
pp. 125-133 ◽  
Author(s):  
Antonio Qualtieri ◽  
Maria Le Pera ◽  
Elena Urso ◽  
Francesco Bono ◽  
Paola Valentino ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Peripheral Nerve ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

scholarly journals Optimized sample preparation for two-dimensional gel electrophoresis of soluble proteins from chicken bursa of Fabricius

2009 ◽  
Vol 7 (1) ◽  
pp. 38 ◽  
Author(s):  
Yongping Wu ◽  
Jiyong Zhou ◽  
Xin Zhang ◽  
Xiaojuan Zheng ◽  
Xuetao Jiang ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Gel Electrophoresis ◽  
Soluble Proteins ◽  
Bursa Of Fabricius ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

"High-resolution" mini-two-dimensional gel electrophoresis automatically run and stained in less than 6 h with small, ready-to-use slab gels.

1988 ◽  
Vol 34 (1) ◽  
pp. 166-170 ◽  
Author(s):  
D Hochstrasser ◽  
V Augsburger ◽  
T Pun ◽  
D Weber ◽  
C Pellegrini ◽  
...  
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Plasma Sample ◽  
Clinical Chemistry ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Automatic Computer ◽  
Clinical Laboratories ◽  
Highly Sensitive ◽  
Slab Gels

Abstract Although two-dimensional (2-D) gel electrophoresis is one of the most powerful techniques for analyzing protein mixtures, its application in routine clinical laboratories is currently limited, because it is time-consuming, complex, and relatively expensive. Here we describe a method for automatically running and staining "high-resolution" mini 2-D electrophoresis gels in less than 6 h, by using "ready-to-use" slab gels and a PhastSystem electrophoresis apparatus. We present 2-D gel electrophoretograms of 25 nL of plasma, as well as their automatic computer analysis. For comparison, a conventional 2-D gel electrophoresis profile of 200 nL of a plasma sample is shown. The technique is easy to perform, highly sensitive, rapid, and potentially useful in semi-routine clinical chemistry laboratories.

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