Assembly and Characterization of Biomolecule–Gold Nanoparticle Conjugates and Their Use in Intracellular Imaging

2005 ◽  
pp. 085-100 ◽  
Author(s):  
Alexander Tkachenko ◽  
Huan Xie ◽  
Stefan Franzen ◽  
Daniel L. Feldheim
Keyword(s):  
Gold Nanoparticle ◽  
Intracellular Imaging

Synthesis and characterization of a sulfur-containing phthalocyanine-gold nanoparticle hybrid

2015 ◽  
Vol 19 (01-03) ◽  
pp. 335-343 ◽  
Author(s):  
Vicente M. Blas-Ferrando ◽  
Javier Ortiz ◽  
Fernando Fernández-Lázaro ◽  
Ángela Sastre-Santos
Keyword(s):  
Gold Nanoparticle ◽  
Lipoic Acid ◽  
Zinc Phthalocyanine ◽  
Synthesis And Characterization ◽  
Ligand Exchange Reaction ◽  
H Nmr ◽  
Fluorescence Measurements ◽  
Transmission Electron ◽  
Yield Values

This work reports on the synthesis and characterization of a new gold nanoparticle-zinc phthalocyanine system, AuNP - S (t Bu )3 ZnPc , prepared by a ligand exchange reaction of tetraoctylammonium bromide with a novel unsymmetrically substituted zinc phthalocyanine which contains one thioester group in the peripheral position [ AcS (t Bu )3 ZnPc ]. The AuNP - S (t Bu )3 ZnPc hybrid was characterized using UV-vis and 1 H NMR spectroscopies. Transmission electron microscopy allowed the estimation of the size, which was calculated to be ~5 nm. AuNPs - S (t Bu )3 ZnPc conjugate showed much lower fluorescence quantum yield values than the AcS (t Bu )3 ZnPc demonstrating either an energy or electron transfer from the ZnPc to the AuNP . The AuNP - S (t Bu )3 ZnPc hybrid has been anchored to a TiO 2 semiconducting layer using lipoic acid. A solid configuration of TiO 2-lipoic acid- AuNP - S (t Bu )3 ZnPc has been prepared by anchoring lipoic acid to the TiO 2 ( TiO 2-LA) and introducing later the TiO 2-LA with free thiol groups in a toluene solution of AuNP - S (t Bu )3 ZnPc . We have also observed by UV-vis and fluorescence measurements the importance of the ZnPc in avoiding AuNP aggregation on the TiO 2 surface.

Thermodynamics of Adsorption of Alcohol Dehydrogenase on Gold Nanoparticle Surface: A Model Based Analysis Versus Direct Measurement

2021 ◽  
Author(s):  
Akriti Mishra ◽  
Kamini Mishra ◽  
Dipayan Bose ◽  
Abhijit Chakrabarti ◽  
Puspendu Kumar Das
Keyword(s):  
Alcohol Dehydrogenase ◽  
Gold Nanoparticle ◽  
Protein Interaction ◽  
Protein Corona ◽  
Quality Data ◽  
Nanoparticle Surface ◽  
Tremendous Importance ◽  
Good Quality Data ◽  
Model Based Analysis

Characterization of nanoparticle protein corona has gained tremendous importance lately. The parameters which quantitatively establish a specific nanoparticle-protein interaction need to be measured accurately since good quality data is necessary...

scholarly journals Characterization of Conventional One-Step Sodium Thiosulfate Facilitated Gold Nanoparticle Synthesis

2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Scott-Eugene Saverot ◽  
Laura M Reese ◽  
Daniela Cimini ◽  
Peter J Vikesland ◽  
Lissett Ramirez Bickford
Keyword(s):  
Gold Nanoparticle ◽  
Sodium Thiosulfate ◽  
Nanoparticle Synthesis ◽  
One Step ◽  
Gold Nanoparticle Synthesis

The Synthesis and Characterization of PEG-SH-Modified Gold Nanoparticle in One-Pot Synthesis by Stenotrophomonas maltophilia

2019 ◽  
Vol 19 (11) ◽  
pp. 7278-7284
Author(s):  
Jian-Yuan Huang ◽  
Min-Hua Chen ◽  
Feng-Huei Lin
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Stenotrophomonas Maltophilia ◽  
Culture Medium ◽  
Synthesis And Characterization ◽  
One Pot ◽  
Residual Solvent ◽  
Green Route ◽  
The Relationship

Colloidal gold nanoparticles have been researched and utilized in many technical applications. However, the conventionalmethods to produce polyethyleneglycol (PEG) immobilized gold nanoparticles have to take several steps, including residual solvent removing. In the study, we propose an idea green route to synthesize gold nanoparticles by using Stenotrophomonas maltophilia in a one-pot reaction. The relationship between Au precursor and S. maltophilia was evaluated systematically. After PEG-SH addition, the bacterial cell wall was broken down and the synthetic nanoparticles could be released into culture medium. Furthermore, we identified that the crystal structure of synthetic gold nanoparticle was face-center cubic and PEG-SH was immobilized on synthetic gold nanoparticle ideally. The size of Au-PEG-SH was smaller than 30 nm. These findings suggest that gold nanoparticle with PEG-SH modification could be prepared in an eco-friendly one-pot reaction through the metabolic activity of S. maltophilia.

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