Gibberellic Acid Production by Solid-State Fermentation in Coffee Husk

Gibberellic acid production using Fusarium moniliforme, isolated from wilted sugarcane plant has been investigated by solid state fermentation (SSF). The gibberellic acid production of 154mgm/gm was obtained on commercial wheat bran (CWB) mineral salt acid bed in 500 ml flasks after 168 h incubation. The gibberellic acid production rate was about 0.6 to 0.9 mgm/gm/hr during 96 to 168 h. Different carbon sources namely sucrose, lactose, maltose, soluble starch, glycerol, wheat flour and maize flour were tested as an additional substrate along with CWB at the concentration of 25% w/w or v/w base to observe its effects on gibberellic acid production. Soluble starch has been proved the best additional carbon source for gibberellic acid production, which yielded 1160mgm/gm of gibberellic acid after 168 h. Similarly, various nitrogen sources namely NH4Cl, NH4NO3, (NH4)2SO4, (NH4)MoO4 and urea were tested as an additional substrate at the concentration of 0.07% w/w of CWB. Urea was proved as the best nitrogen source which yielded 532 mgm/gm of gibberellic acid after 168 h incubation. We have observed about 7.5-fold and 3.5-fold increase in gibberellic acid production upon addition of soluble starch and urea respectively, in CWB using Fusarium moniliforme.Int J Appl Sci Biotechnol, Vol 4(3): 402-407

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Effect of water activity on gibberellic acid production by Gibberella fujikuroi under solid-state fermentation conditions

Process Biochemistry ◽

10.1016/j.procbio.2004.11.008 ◽

2005 ◽

Vol 40 (8) ◽

pp. 2655-2658 ◽

Cited By ~ 27

Author(s):

Andrés Corona ◽

Doris Sáez ◽

Eduardo Agosin

Keyword(s):

Solid State ◽

Gibberellic Acid ◽

Water Activity ◽

Solid State Fermentation ◽

Acid Production ◽

Gibberella Fujikuroi ◽

Fermentation Conditions ◽

Effect Of Water

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Solid state fermentation: physical and nutritional factors influencing gibberellic acid production

Applied Microbiology and Biotechnology ◽

10.1007/bf00166770 ◽

1990 ◽

Vol 34 (2) ◽

pp. 145-148 ◽

Cited By ~ 22

Author(s):

P. K. R. Kumar ◽

B. K. Lonsane

Keyword(s):

Solid State ◽

Gibberellic Acid ◽

Solid State Fermentation ◽

Acid Production ◽

Nutritional Factors ◽

Factors Influencing

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Kinetics of Gibberella fujikuroi Growth and Gibberellic Acid Production by Solid-State Fermentation in a Packed-Bed Column Bioreactor

Biotechnology Progress ◽

10.1021/bp049819x ◽

2004 ◽

Vol 20 (5) ◽

pp. 1449-1453 ◽

Cited By ~ 18

Author(s):

C.M.M. Machado ◽

B.O. Oishi ◽

A. Pandey ◽

C.R. Soccol

Keyword(s):

Solid State ◽

Gibberellic Acid ◽

Solid State Fermentation ◽

Acid Production ◽

Packed Bed ◽

Gibberella Fujikuroi ◽

Packed Bed Column ◽

Column Bioreactor ◽

Kinetics Of

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A new alternative to produce gibberellic acid by solid state fermentation

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132009000700023 ◽

2009 ◽

Vol 52 (spe) ◽

pp. 181-188 ◽

Cited By ~ 14

Author(s):

Cristine Rodrigues ◽

Luciana Porto de Souza Vandenberghe ◽

Juliana Teodoro ◽

Juliana Fraron Oss ◽

Ashok Pandey ◽

...

Keyword(s):

Solid State ◽

Gibberellic Acid ◽

Solid State Fermentation ◽

Growth And Development ◽

Production Costs ◽

Coffee Husk ◽

Inoculum Production ◽

Cassava Bagasse ◽

Inoculum Preparation ◽

Citric Pulp

Gibberellic acid (GA3) is an important hormone, which controls plant's growth and development. Solid State Fermentation (SSF) allows the use of agro-industrial residues reducing the production costs. The screening of strains (four of Gibberella fujikuoroi and one of Fusarium moniliforme) and substrates (citric pulp, soy bran, sugarcane bagasse, soy husk, cassava bagasse and coffee husk) and inoculum preparation study were conducted in order to evaluate the best conditions to produce GA3 by SSF. Fermentation assays were carried out in erlenmeyers flasks at 29°C, with initial moisture of 75-80%. Different medium for inoculum production were tested in relation to cells viability and GA3 production by SSF. F. moniliforme LPB 03 and citric pulp were chosen for GA3 production. The best medium for inoculum production was citric pulp extract supplemented with sucrose. GA3 production by SSF reached 5.9 g /kg of dry CP after 3 days of fermentation.

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