A new alternative to produce gibberellic acid by solid state fermentation

Gibberellic acid (GA3) is an important hormone, which controls plant's growth and development. Solid State Fermentation (SSF) allows the use of agro-industrial residues reducing the production costs. The screening of strains (four of Gibberella fujikuoroi and one of Fusarium moniliforme) and substrates (citric pulp, soy bran, sugarcane bagasse, soy husk, cassava bagasse and coffee husk) and inoculum preparation study were conducted in order to evaluate the best conditions to produce GA3 by SSF. Fermentation assays were carried out in erlenmeyers flasks at 29°C, with initial moisture of 75-80%. Different medium for inoculum production were tested in relation to cells viability and GA3 production by SSF. F. moniliforme LPB 03 and citric pulp were chosen for GA3 production. The best medium for inoculum production was citric pulp extract supplemented with sucrose. GA3 production by SSF reached 5.9 g /kg of dry CP after 3 days of fermentation.

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Solid-State Fermentation in Brewer’s Spent Grains by Fusarium fujikuroi for Gibberellic Acid Production

Biointerface Research in Applied Chemistry ◽

10.33263/briac115.1304213052 ◽

2021 ◽

Vol 11 (5) ◽

pp. 13042-13052

Keyword(s):

Solid State ◽

Moisture Content ◽

Gibberellic Acid ◽

Solid State Fermentation ◽

Production Costs ◽

Fusarium Fujikuroi ◽

New Production ◽

Fermentation Time ◽

Spent Grains ◽

Biotechnological Processes

The search for new production methodologies of gibberellic acid (GA3), such as solid-state fermentation (SSF), and the use of agro-industrial waste are important to lower production costs. Therefore, the aim of this study was GA3 production by Fusarium fujikuroi on SSF mode using brewer’s spent grains (BSG). BSG presents in its composition components that are known to be excellent inducers of metabolite production, showing, this way, its potential to be used as the substrate in biotechnological processes. Optimization of GA3 production was carried out using a 22 central composite design, considering the effects of moisture content, temperature, and fermentation time. The highest mycelial growth and GA3 production (0.82 g.Kg-1) was obtained in the condition of 80% moisture content, 28 °C in 96 hours of fermentation. These results suggest that the SSF using BSG as the medium for the growth of F. fujikuroi is a viable way to GA3 produce.

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Gibberellic Acid Production by Solid-State Fermentation in Coffee Husk

Applied Biochemistry and Biotechnology ◽

10.1385/abab:102-103:1-6:179 ◽

2002 ◽

Vol 102-103 (1-6) ◽

pp. 179-192 ◽

Cited By ~ 32

Author(s):

Cristina M M Machado ◽

Carlos R Soccol ◽

Brás H De Oliveira ◽

Ashok Pandey

Keyword(s):

Solid State ◽

Gibberellic Acid ◽

Solid State Fermentation ◽

Acid Production ◽

Coffee Husk

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Gibberellic acid production by Gibberella fujikuroi under solid-state fermentation of cassava bagasse and rice hull

Journal of Biotechnology ◽

10.1016/j.jbiotec.2008.07.853 ◽

2008 ◽

Vol 136 ◽

pp. S371 ◽

Cited By ~ 1

Author(s):

Ángela M. Otálvaro ◽

Germán D. Gutiérrez ◽

Diego A. Pierotty ◽

Fabián A. Parada ◽

Néstor A. Algecira

Keyword(s):

Solid State ◽

Gibberellic Acid ◽

Solid State Fermentation ◽

Acid Production ◽

Gibberella Fujikuroi ◽

Rice Hull ◽

Cassava Bagasse

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Towards a competitive solid state fermentation: Cellulases production from coffee husk by sequential batch operation and role of microbial diversity

The Science of The Total Environment ◽

10.1016/j.scitotenv.2017.02.184 ◽

2017 ◽

Vol 589 ◽

pp. 56-65 ◽

Cited By ~ 32

Author(s):

Alejandra Cerda ◽

Teresa Gea ◽

M Carmen Vargas-García ◽

Antoni Sánchez

Keyword(s):

Solid State ◽

Microbial Diversity ◽

Solid State Fermentation ◽

Batch Operation ◽

Coffee Husk

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Study of Gibberellic Acid Production by Solid State Fermentation Using Fusarium Moniliforme Sheldon

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v4i3.15588 ◽

2016 ◽

Vol 4 (3) ◽

pp. 402-407 ◽

Cited By ~ 3

Author(s):

Rakeshkumar Ramanlal Panchal ◽

Piyushbhai Vishnubhai Desai

Keyword(s):

Solid State ◽

Gibberellic Acid ◽

Solid State Fermentation ◽

Acid Production ◽

Fusarium Moniliforme ◽

Carbon Sources ◽

Nitrogen Sources ◽

Fold Increase ◽

Soluble Starch ◽

Sugarcane Plant

Gibberellic acid production using Fusarium moniliforme, isolated from wilted sugarcane plant has been investigated by solid state fermentation (SSF). The gibberellic acid production of 154mgm/gm was obtained on commercial wheat bran (CWB) mineral salt acid bed in 500 ml flasks after 168 h incubation. The gibberellic acid production rate was about 0.6 to 0.9 mgm/gm/hr during 96 to 168 h. Different carbon sources namely sucrose, lactose, maltose, soluble starch, glycerol, wheat flour and maize flour were tested as an additional substrate along with CWB at the concentration of 25% w/w or v/w base to observe its effects on gibberellic acid production. Soluble starch has been proved the best additional carbon source for gibberellic acid production, which yielded 1160mgm/gm of gibberellic acid after 168 h. Similarly, various nitrogen sources namely NH4Cl, NH4NO3, (NH4)2SO4, (NH4)MoO4 and urea were tested as an additional substrate at the concentration of 0.07% w/w of CWB. Urea was proved as the best nitrogen source which yielded 532 mgm/gm of gibberellic acid after 168 h incubation. We have observed about 7.5-fold and 3.5-fold increase in gibberellic acid production upon addition of soluble starch and urea respectively, in CWB using Fusarium moniliforme.Int J Appl Sci Biotechnol, Vol 4(3): 402-407

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