Painting Beyond Itself: The Medium in the Post-Medium Condition, Isabella Graw and Ewa Lajer-Burcharth (eds) (2016)

2019 ◽  
Vol 5 (2) ◽  
pp. 313-315
Author(s):  
Alison Green
Keyword(s):  
Medium Condition

Review of: Painting Beyond Itself: The Medium in the Post-Medium Condition, Isabella Graw and Ewa Lajer-Burcharth (eds) (2016)Berlin: Sternberg Press, 292 pp.,ISBN 978-3-95679-007-2, p/bk, £13.95

Studies in flock mating of sheep. 3. Effect of undernutrition of ewes during joining

1971 ◽  
Vol 11 (51) ◽  
pp. 375 ◽  
Author(s):  
AWH Braden
Keyword(s):  
Genital Tract ◽  
Mating Behaviour ◽  
Fertilization Rate ◽  
Early Embryo ◽  
Ovulation Rate ◽  
Medium Condition ◽  
Under Nutrition ◽  
Embryonic Loss ◽  
The Mean

One hundred five-year-old Merino ewes in medium condition (44 kg) were fed at one-third of the maintenance level from the start of joining until 3-4 weeks post-coitum (P.C.). The mean decrease in liveweight in the 3-4 weeks was 1.5 kg/week. One hundred similar ewes were fed so as to maintain liveweight. A ram was joined with each group during daylight hours for 15 days, and the course of mating observed constantly. Ewes were killed either 1-2 days p.c. or 24-38 days p.c. There was no evidence of an effect of the under-nutrition on mating behaviour, number of spermatoza in the ewe genital tract, fertilization rate (89 per cent for both) or early embryo losses (7 per cent and 3 per cent). The relatively low embryonic loss appeared to be related to the low ovulation rate (1.16).

scholarly journals A lipid-free and insulin-supplemented medium supports de novo fatty acid synthesis gene activation in melanoma cells

2018 ◽  
Author(s):  
Su Wu ◽  
Anders M. Näär
Keyword(s):  
Cell Culture ◽  
Fatty Acid ◽  
Cell Lines ◽  
Melanoma Cell ◽  
De Novo ◽  
Melanoma Cells ◽  
Controlled Study ◽  
Gene Promoters ◽  
Medium Condition ◽  
Melanoma Cell Lines

AbstractWhile investigating the role played by de novo lipid (DNL) biosynthesis in cancer cells, we sought a medium condition that would support cell proliferation without providing any serum lipids. Here we report that a defined serum free cell culture medium condition containing insulin, transferrin and selenium (ITS) supports controlled study of transcriptional regulation of de novo fatty acid (DNFA) production and de novo cholesterol synthesis (DNCS) in melanoma cell lines. This lipid-free ITS medium is able to support continuous proliferation of several melanoma cell lines that utilize DNL to support their lipid requirements. We show that the ITS medium stimulates gene transcription in support of both DNFA and DNCS, specifically mediated by SREBP1/2 in melanoma cells. We further found that the ITS medium promoted SREBP1 nuclear localization and occupancy on DNFA gene promoters. Our data show clear utility of this serum and lipid-free medium for melanoma cancer cell culture and lipid-related areas of investigation.

scholarly journals Workshop: Making Beyond the Post-Medium Condition

2017 ◽  
Vol 18 (2) ◽  
pp. 133-162
Author(s):  
Christine Ellison
Keyword(s):  
Medium Condition
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