scholarly journals Genetic variation and phylogenetic analysis of Indonesian indigenous catfish (baung fish) based on mitochondrial 12S rRNA gene

2021 ◽  
Vol 14 (3) ◽  
pp. 751-757
Author(s):  
Rini Widayanti ◽  
Ken Ayik Kusumaastuti ◽  
Joana Martha Novi ◽  
Fadila Khairuna Adani ◽  
Catrine Relia Patrecia Gultom ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Phylogenetic Relationships ◽  
Population Based ◽  
Genetic Distances ◽  
12S Rrna ◽  
Rrna Gene ◽  
Mystus Vittatus ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna

Background and Aim: Baung fish is an essential commodity in Indonesia; however, few studies have explored the genetic diversity of Indonesian catfish. Thus, this study aimed to analyze the genetic variation and phylogenetic relationships among Indonesian catfish based on the mitochondrial 12S ribosomal RNA (rRNA) gene. Materials and Methods: In total, 28 catfish were collected from nine rivers in seven provinces and from the Indian Ocean. Catfish genomes were obtained from epaxial and hepaxial muscle samples. The mitochondrial 12S rRNA gene was amplified by polymerase chain reaction using a pair of primers (Baung12SF and Baung12SR). The 12S rRNA sequences were analyzed using MEGA X to determine genetic variation and phylogenetic relationships. Results: In total, 178 variation sites in the 12S rRNA gene were substituted among Indonesian catfish. The genetic distance between all Indonesian catfish samples was 0.1-16.0%. The closest genetic distance was between MP and PM catfish, whereas the farthest genetic distances were between BF and EM and PD and EM. For the entire population, based on mean diversity calculations, the number of base substitutions per site was 0.08. Conclusion: Indonesian catfish were divided into four clades based on the 12S rRNA gene. The catfish MP, KR, PM, MS, BB, and KS were grouped with Hemibagrus nemurus, the catfish EM was grouped with Mystus vittatus, the catfish BSBJ was grouped with Pangasius pangasius, and the catfish PD and BF were grouped with Netuma thalassina.

Phylogenetic Relationships among the Species of the Genus Testudo (Testudines: Testudinidae) Inferred from Mitochondrial 12S rRNA Gene Sequences

2002 ◽  
Vol 22 (2) ◽  
pp. 174-183 ◽  
Author(s):  
Antoinette C. van der Kuyl ◽  
Donato L. Ph. Ballasina ◽  
John T. Dekker ◽  
Jolanda Maas ◽  
Ronald E. Willemsen ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
12S Rrna ◽  
Rrna Gene ◽  
Gene Sequences ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna

scholarly journals Phylogenetic relationships of snails of the genera Oncomelania and Tricula inferred from the mitochondrial 12S rRNA gene

2003 ◽  
Vol 31 (1) ◽  
pp. 5-10 ◽  
Author(s):  
MUNEHIRO OKAMOTO ◽  
CHIN-TSON LO ◽  
WILFRED U. TIU ◽  
DONGCHUAN QUI ◽  
PINARDI HADIDJAJA ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
12S Rrna ◽  
Rrna Gene ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna

Assessing the phylogenetic signal of the nuclear β-Fibrinogen intron 7 in salamandrids (Amphibia: Salamandridae)

2006 ◽  
Vol 27 (3) ◽  
pp. 409-418 ◽  
Author(s):  
Fernando Sequeira ◽  
Nuno Ferrand ◽  
D. James Harris
Keyword(s):  
Phylogenetic Relationships ◽  
Phylogenetic Signal ◽  
12S Rrna ◽  
Rrna Gene ◽  
Length Variation ◽  
Β Subunit ◽  
Phylogenetic Studies ◽  
12S Rrna Gene ◽  
Considerable Length ◽  
Mitochondrial 12S Rrna

AbstractThe potential of the seventh intron of the β-subunit of the fibrinogen gene (β-fibint 7) for phylogenetic analysis within the Salamandridae family was explored, comparing the topologies of trees based on this marker to those based on mitochondrial 12S rRNA gene previously published. Using primers designed specifically for amphibians, we amplified 25 sequences of β-fibint 7 corresponding to 15 species of salamandrids and one plethodontid species. There was considerable length variation among the β-fibint 7 sequences examined, ranging from 1123 bp in S. atra to 400 bp in P. waltl. Many aspects of the phylogenetic relationships estimated by the two independent loci were congruent and corroborate current taxonomic hypothesis. Although the number of taxa analysed is small, the data obtained in this work suggested that β-fibint 7 is a useful marker for assessing phylogenetic relationships within the Salamandridae family, and is probably appropriate for phylogenetic studies among closely related salamanders that have diverged over the last 20 Myr.

scholarly journals Phylogenetic Relationships Between Zokors Myospalax (Mammalia, Rodentia) Determined on the Basis of Morphometric and Molecular Analyses

2020 ◽  
Vol 74 (1) ◽  
pp. 25-34
Author(s):  
Dalius Butkauskas ◽  
Marija Starodubaitė ◽  
Mikhail Potapov ◽  
Olga Potapova ◽  
Sergei Abramov ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
Molecular Data ◽  
12S Rrna ◽  
Rrna Gene ◽  
Novosibirsk Oblast ◽  
Geographic Isolation ◽  
Morphological Adaptations ◽  
12S Rrna Gene ◽  
Genetic Features ◽  
Mitochondrial 12S Rrna

AbstractPhylogenetic relationships between zokors living in different territories of Russia: Altai zokors Myospalax myospalax from “Altai” (Altai Republic and Altaiskii Krai) and “Priobie” from the River Ob zone (Tomsk oblast and Novosibirsk oblast) and subspecies M. m. tarbagataicus from Kazakhstan (ridge Tarbagatai) and M. aspalax and M. psilurus from Zabaikalskii Krai were determined on the basis of craniometrical and molecular analysis of the mitochondrial 12S rRNA gene. The comparison of the craniometrical and molecular data revealed significant differences between the Altai zokors of the “Priobie” (the River Ob zone) and “Altai” populations. The importance of geographic isolation to the formation of morphometric and genetic differentiation of distinct geographic forms of the investigated zokors is shown. Specific ecological and morphological adaptations and distinct genetic features of two forms of zokors indicate the existence of separate subspecies of the species M. myospalax.

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