Genetic variation and phylogenetic analysis of Indonesian indigenous catfish (baung fish) based on mitochondrial 12S rRNA gene

Background and Aim: Baung fish is an essential commodity in Indonesia; however, few studies have explored the genetic diversity of Indonesian catfish. Thus, this study aimed to analyze the genetic variation and phylogenetic relationships among Indonesian catfish based on the mitochondrial 12S ribosomal RNA (rRNA) gene. Materials and Methods: In total, 28 catfish were collected from nine rivers in seven provinces and from the Indian Ocean. Catfish genomes were obtained from epaxial and hepaxial muscle samples. The mitochondrial 12S rRNA gene was amplified by polymerase chain reaction using a pair of primers (Baung12SF and Baung12SR). The 12S rRNA sequences were analyzed using MEGA X to determine genetic variation and phylogenetic relationships. Results: In total, 178 variation sites in the 12S rRNA gene were substituted among Indonesian catfish. The genetic distance between all Indonesian catfish samples was 0.1-16.0%. The closest genetic distance was between MP and PM catfish, whereas the farthest genetic distances were between BF and EM and PD and EM. For the entire population, based on mean diversity calculations, the number of base substitutions per site was 0.08. Conclusion: Indonesian catfish were divided into four clades based on the 12S rRNA gene. The catfish MP, KR, PM, MS, BB, and KS were grouped with Hemibagrus nemurus, the catfish EM was grouped with Mystus vittatus, the catfish BSBJ was grouped with Pangasius pangasius, and the catfish PD and BF were grouped with Netuma thalassina.

Status of Ornamental Fish Diversity of Urpod Beel of Goalpa-ra District, Assam

The North Eastern Sates including Assam are very rich in aquatic bio-resources and dominated by several endemic fish resources. The study was conducted from June 2018- July 2019 in two different fish assembling villages viz. Chamaguri (W) and Garokuta (North) of Urpad Beel and fish species were recorded along with their IUCN Conservation status. Altogether, 31 ornamental fish species belonging to 16 Genera, 15 Families, and 6 Orders be-longing to orders like Beloniformes, Cypriniformes, Osteoglossiformes, Siluriformes, Sym-branchiformes, Perciformes, Tetradontiformes were recorded. Out of the recorded fish spe-cies, Lepidocephalichthys guntea (Hamilton-Buchanan,1822), Channa gachua (Hamilton, 1822) and Chanda nama (Hamilton, 1822) were assessed as NE, Botia rostrata (Gun-ther,1868) and Channa gachua (Hamilton, 1822), Mystus vittatus ( Bloch, 1794) as VU, Ctenops nobilis (Mc Clelland, 1845) as NT as per IUCN Status 2013. The ornamental fishes are found in the weed assemblage of the Beel including Eichhornia crassipes, Hydrilla verticillate and Ceratophyllum demersum.