scholarly journals THE INFLUENCE OF CULTIVATION CONDITIONS ON MORPHOPHYSIOLOGICAL ACTIVITY AND CONTENT OF PHENOLIC COMPOUNDS OF ST. JOHN'S WORT (HYPERICUM PERFORATUM L.) IN VITRO CULTURE

2018 ◽  
pp. 223-229
Author(s):  
Вера (Vera) Николаевна (Nikolaevna) Овчинникова (Ovchinnikova) ◽  
Наталья (Natal'ya) Петровна (Petrovna) Карсункина (Karsunkina) ◽  
Петр (Petr) Николаевич (Nikolaevich) Харченко (Kharchenko) ◽  
Наталья (Natal'ya) Владимировна (Vladimirovna) Никифорова (Nikiforova)
Keyword(s):  
Phenolic Compounds ◽  
Hypericum Perforatum ◽  
Spectral Composition ◽  
In Vitro Cultivation ◽  
Light Spectrum ◽  
St John’S Wort ◽  
St John's Wort ◽  
Hypericum Perforatum L ◽  
Number Of Shoots

The paper discusses the joint effect of cytokine 6-BAP and different spectra when illuminated by LEDs on the morphological parameters of growth, development and the content of phenolic compounds in plants of St. John's Wort (Hypericum perforatum L.) of two genotypes – wild and cultivated (cultivar Zolotodolinskiy) – in conditions of in vitro cultivation.It is shown that the light of different spectral composition and the hormonal composition of medium influences the morphogenesis, the productivity of biomass and the synthesis of phenolic compounds by plants under in vitro cultivation conditions.It is established that the combination of the light spectrum, and the hormonal composition of nutrient medium may substantially increase the content of soluble phenolic compounds in both wild and cultural genotypes of Hypericum.The analysis of the obtained results shows a direct correlation dependence of the content of phenolic compounds on the number of shoots and their mass. The correlation between the number of shoots and the content of phenolic compounds has a direct character and strongly expressed.

scholarly journals Development of a Micropropagation Protocol for St. John's Wort (Hypericum perforatum L.)

HortScience ◽  
2002 ◽  
Vol 37 (6) ◽  
pp. 978-980 ◽  
Author(s):  
Joe-Ann McCoy ◽  
N.D. Camper
Keyword(s):  
Depressive Disorders ◽  
Hypericum Perforatum ◽  
Leaf Disc ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
St John’S Wort ◽  
St John's Wort ◽  
Explant Source ◽  
Hypericum Perforatum L

Hypericum perforatum L. (St. John's Wort) has an extensive history as an important medicinal herb used for the treatment of neurological and depressive disorders (Linde et al., 1996). The objective of this study was to establish an in vitro tissue culture protocol for St. John's Wort. Nodal segments, axillary buds, and leaf disc explants produced multiple shoots and callus on Murashige and Skoog minimal organics medium supplemented with combinations of indoleacetic acid (IAA; 0.57, 2.85, 5.71 μm) and benzylaminopurine (BA; 2.22, 4.44, 8.88 μm). Shoot production occurred on all combinations of IAA/BA tested and was significantly less in treatments without hormones. Callus production was higher on treatments containing 2.85 μm IAA + 4.44 μm BA, or 5.71 μm IAA + 8.88 μm BA. Shoots transferred to hormone-free medium at 8 weeks formed roots by 12 weeks. A micropropagation protocol was established for St. John's Wort using mature plants as the explant source.

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