scholarly journals Preliminary results of studying the genetic diversity of Malaxis monophyllos (Orchidaceae) in the Amur region

2021 ◽  
Vol 20 (1) ◽  
pp. 429-432
Author(s):  
E. I. Terentieva ◽  
G. V. Degtjareva ◽  
S. V. Efimov ◽  
T. I. Varlygina
Keyword(s):  
Morphological Characters ◽  
Amur Region ◽  
Nuclear Ribosomal Dna ◽  
Large Area ◽  
Molecular Phylogenetic Tree ◽  
Molecular Studies ◽  
Molecular Phylogenetic ◽  
Number Of Leaves ◽  
Herbarium Collections ◽  
Its1 And Its2

For research the plants of Malaxis monophyllos (L.) Sw. were sampled in the Amur region and takenfrom the herbarium collections of the same region (MW and MHA). The plants of M. monophyllos have a high degreeof vari-ability of quantitative morphological characters. First, we paid attention to the number of leaves, the height ofplants and peduncles, the number and size of leaves and the number of flowers. Therefore, the purpose of this workwas compara-tive molecular studies of individuals of the same species with different morphological characteristics.Internal transcribed spacers (ITS1 and ITS2) of the 18S–26S region of nuclear ribosomal DNA were chosen as amolecular marker. On the molecular phylogenetic tree, the analyzed M. monophyllos plants form clade with a highposterior probability (1.0). The ITS sequences of the analyzed samples are not identical. The level of geneticdifferentiation of nucleotide sequences was 0.12 %, which corresponds to the variability within the species, whichconfirms the belonging of the analyzed samples to the same species. Since the species has a large area, it is necessary toanalyze plant samples from other regions to obtain reliable results. The different regions of the nuclear and plastidgenomes should be added to the genetic analysis.

scholarly journals Phylogeny of the Labeoninae (Teleostei, Cypriniformes) based on nuclear DNA sequences and implications on character evolution and biogeography

2012 ◽  
Vol 58 (6) ◽  
pp. 837-850 ◽  
Author(s):  
Lanping Zheng ◽  
Junxing Yang ◽  
Xiaoyong Chen
Keyword(s):  
Molecular Phylogeny ◽  
Dna Sequences ◽  
Nuclear Dna ◽  
Morphological Characters ◽  
Character Evolution ◽  
Lower Lip ◽  
Molecular Phylogenetic Tree ◽  
Systematic Exploration ◽  
Molecular Phylogenetic ◽  
History Of

Abstract The Labeoninae is a subfamily of the family Cyprinidae, Order Cypriniformes. Oromandibular morphology within the Labeoninae is the greatest among cyprinid fishes. Although several phylogenetic studies about labeonines have been undertaken the results have been inconsistent and a comprehensive phylogeny is needed. Further, an incongruence between morphological and molecular phylogeny requires a systematic exploration of the significance of morphological characters on the basis of the molecular phylogeny. In this study, a total of 292 nucleotide sequences from 73 individuals (representing 24 genera and 73 species) of Labeoninae were analyzed. The results of the phylogenetic analysis indicate that there are four major clades within Labeoninae and three monophyletic lineages within the fourth clade. Results of the character evolution show that all oroman-dibular morphological characters are homoplastically distributed on the molecular phylogenetic tree and suggests that these characters evolved several times during the history of labeonines. In particular, the labeonine , a specific disc on the lower lip, has been acquired three times and reversed twice. These morphological characters do not have systematic significance but can be useful for taxonomy. The results of biogeography suggest that the Labeoninae originated from Southeast Asia and separately dispersed to Africa, East Asia and South Asia.

Pseudosperma albobrunneum sp. nov. from coniferous forests of Pakistan

Mycotaxon ◽  
2021 ◽  
Vol 136 (2) ◽  
pp. 361-372
Author(s):  
Sana Jabeen ◽  
Zainab ◽  
Hira Bashir ◽  
Abdul Nasir Khalid
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Its Region ◽  
Morphological Characters ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
Coniferous Forests ◽  
Molecular Phylogenetic ◽  
Khyber Pakhtunkhwa Province ◽  
A New Species

A new species, Pseudosperma albobrunneum, is described and illustrated. The specimens are from different areas of Khyber Pakhtunkhwa province, Pakistan. The identification is based on morphological characters, in combination with molecular phylogenetic analysis of sequences of the ITS region of nuclear ribosomal DNA. The species is found distinct morphologically from all closely related taxa, and molecular data confirm its novelty.

Inocybe pakistanensis, a new species in section Rimosae s. str. from Pakistan

Phytotaxa ◽  
2018 ◽  
Vol 348 (4) ◽  
pp. 279 ◽  
Author(s):  
ZIA ULLAH ◽  
SANA JABEEN ◽  
HABIB AHMAD ◽  
ABDUL NASIR KHALID
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Morphological Characters ◽  
Nuclear Ribosomal Dna ◽  
Molecular Phylogenetic Analysis ◽  
Its Regions ◽  
Molecular Phylogenetic ◽  
A New Species

Inocybe pakistanensis is described and illustrated as a new species based on morphological characters and molecular phylogenetic analysis of nuclear ribosomal DNA including the internal transcribed spacer (ITS) regions along with larger subunit (LSU). The distinctive basidiomata have a highly rimose and fibrillose golden brown pileus with a reddish brown, prominent umbo; ellipsoid to amygdaliform, slightly phasoeliform smooth basidiospores; and clamped septa in all the tissues. Molecular phylogenetic analysis supports the placement of I. pakistanensis in section Rimosae s. str.

scholarly journals A multi-gene phylogeny of Chlorophyllum (Agaricaceae, Basidiomycota): new species, new combination and infrageneric classification

MycoKeys ◽  
2018 ◽  
Vol 32 ◽  
pp. 65-90 ◽  
Author(s):  
Zai-Wei Ge ◽  
Adriaana Jacobs ◽  
Else C. Vellinga ◽  
Phongeun Sysouphanthong ◽  
Retha van der Walt ◽  
...  
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Nuclear Ribosomal Dna ◽  
New Combination ◽  
Infrageneric Classification ◽  
Molecular Phylogenetic

Taxonomic and phylogenetic studies of Chlorophyllum were carried out on the basis of morphological differences and molecular phylogenetic analyses. Based on the phylogeny inferred from the internal transcribed spacer (ITS), the partial large subunit nuclear ribosomal DNA (nrLSU), the second largest subunit of RNA polymerase II (rpb2) and translation elongation factor 1-α (tef1) sequences, six well-supported clades and 17 phylogenetic species are recognised. Within this phylogenetic framework and considering the diagnostic morphological characters, two new species, C.africanum and C.palaeotropicum, are described. In addition, a new infrageneric classification of Chlorophyllum is proposed, in which the genus is divided into six sections. One new combination is also made. This study provides a robust basis for a more detailed investigation of diversity and biogeography of Chlorophyllum.

Molecular phylogenetic and morphological analyses of the traditional tribe Coriandreae (Umbelliferae-Apioideae)

Phytotaxa ◽  
2015 ◽  
Vol 195 (4) ◽  
pp. 251 ◽  
Author(s):  
Elena I Terenteva ◽  
CARMEN M. VALIEJO-ROMAN ◽  
TAHIR H. SAMIGULLIN ◽  
MICHAEL G. PIMENOV ◽  
PATRICIA M. TILNEY
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Morphological Characters ◽  
Nuclear Ribosomal Dna ◽  
Monophyletic Clade ◽  
Molecular Systematic ◽  
Molecular Phylogenetic ◽  
One New Species ◽  
New Nomenclatural Combinations ◽  
Molecular Trees

All eight genera (Coriandrum, Bifora, Fuernrohria, Schrenkia, Schtschurowskia, Kosopoljanskia, Lipskya and Sclerotiaria) of the tribe Coriandreae (Umbelliferae), delimited on the basis of morphological characters, were studied using molecular systematic methods to check the monophyly of the tribe. Plastid psbA-trnH data contained very little information and were incongruent with nrDNA data. The nrDNA analyses clearly showed that the tribe Coriandreae consists of three separate groups, differing in their sequences of ITS and ETS of nuclear ribosomal DNA. Only Bifora appears to be closely related to Coriandrum, whereas Fuernrohria falls into tribe Careae, and the five remaining taxa, endemic or subendemic to Middle Asia, form a separate clade, informally named the “Schrenkia clade”. The species of Kosopoljanskia do not form a monophyletic clade, being scattered in molecular trees among the Schrenkia species. Kosopoljanskia and Schrenkia are regarded as congeneric. One new species is described and two new nomenclatural combinations are proposed.

Urocystis piptatheri sp. nov. (Urocystaceae), a new species of smut fungus on Piptatherum laterale (Poaceae) from Pakistan

Phytotaxa ◽  
2020 ◽  
Vol 442 (2) ◽  
pp. 80-88
Author(s):  
NAJAM-UL-SAHAR AFSHAN ◽  
AYESHA ANWAR ◽  
MARIA RIAZ ◽  
SIRAJ UD DIN ◽  
ABDUL NASIR KHALID
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Large Subunit ◽  
Morphological Characters ◽  
Spore Wall ◽  
Nuclear Ribosomal Dna ◽  
Smut Fungi ◽  
Large Size ◽  
Molecular Phylogenetic ◽  
A New Species

A new species of smut fungi, Urocystis piptatheri is described from Kumrat Valley, Pakistan based on morphological characters and molecular phylogenetic analysis of nuclear ribosomal DNA comprising the internal transcribed spacer (ITS) regions along with large subunit (LSU). The new species is characterized by the large size of its spore balls, size and number of central spores, spore wall thickness, sori in leaves and by the host plant species (Piptatherum laterale). Phylogenetic analysis of nuclear ribosomal ITS and LSU nucleotide sequences confirmed the novelty of Urocystis piptatheri. A key for the identification of Urocystis species on Pipthatherum spp. is provided.

Transfer of the Kobresia taxa of the flora of Russia and adjacent countries to the genus Carex (Cyperaceae)

2020 ◽  
pp. 125-129
Author(s):  
D. G. Melnikov ◽  
L. I. Krupkina
Keyword(s):  
International Collaboration ◽  
Morphological Characters ◽  
Published Data ◽  
Phylogenetic Studies ◽  
Molecular Phylogenetic ◽  
New Nomenclatural Combinations

Based on the published data of molecular phylogenetic studies of the tribe Cariceae Dumort. genera (Cyperaceae), obtained by an international collaboration (The Global Carex Group, 2016; et al.), and morphological characters of the genera (Kukkonen, 1990; and others), new nomenclatural combinations and replacement names in the genus Carex L. are published for 11 species, one subspecies and two sections previously included in the genus Kobresia Willd.

scholarly journals Disentangling relationships among eastern Mediterranean Cymbalaria including description of a novel species from the southern Peloponnese (Greece)

2021 ◽  
Vol 307 (2) ◽  
Author(s):  
Pau Carnicero ◽  
Núria Garcia-Jacas ◽  
Llorenç Sáez ◽  
Theophanis Constantinidis ◽  
Mercè Galbany-Casals
Keyword(s):  
Eastern Mediterranean ◽  
Phylogenetic Analyses ◽  
Taxonomic Revision ◽  
Distribution Patterns ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Long Distance ◽  
Aegean Islands ◽  
Molecular Phylogenetic

AbstractThe eastern Mediterranean basin hosts a remarkably high plant diversity. Historical connections between currently isolated areas across the Aegean region and long-distance dispersal events have been invoked to explain current distribution patterns of species. According to most recent treatments, at least two Cymbalaria species occur in this area, Cymbalaria microcalyx and C. longipes. The former comprises several intraspecific taxa, treated at different ranks by different authors based on morphological data, evidencing the need of a taxonomic revision. Additionally, some populations of C. microcalyx show exclusive morphological characters that do not match any described taxon. Here, we aim to shed light on the systematics of eastern Mediterranean Cymbalaria and to propose a classification informed by various sources of evidence. We performed molecular phylogenetic analyses using ITS, 3’ETS, ndhF and rpl32-trnL sequences and estimated the ploidy level of some taxa performing relative genome size measures. Molecular data combined with morphology support the division of traditionally delimited C. microcalyx into C. acutiloba, C. microcalyx and C. minor, corresponding to well-delimited nrDNA lineages. Furthermore, we propose to combine C. microcalyx subsp. paradoxa at the species level. A group of specimens previously thought to belong to Cymbalaria microcalyx constitute a well-defined phylogenetic and morphological entity and are described here as a new species, Cymbalaria spetae. Cymbalaria longipes is non-monophyletic, but characterized by being glabrous and diploid, unlike other eastern species. The nrDNA data suggest at least two dispersals from the mainland to the Aegean Islands, potentially facilitated by marine regressions.

Integrative taxonomic and geographic variation analyses in Cyrtodactylus aequalis (Squamata: Gekkonidae) from southern Myanmar (Burma): one species, two different stories

2020 ◽  
Vol 66 (3-4) ◽  
pp. 151-179
Author(s):  
L. Lee Grismer ◽  
L. Wood Perry ◽  
Marta S. Grismer ◽  
Evan S.H. Quah ◽  
Myint Kyaw Thura ◽  
...  
Keyword(s):  
Geographic Variation ◽  
Evolutionary History ◽  
Phylogenetic Inference ◽  
Morphological Characters ◽  
Molecular Data ◽  
Biodiversity Crisis ◽  
Allopatric Populations ◽  
Compelling Argument ◽  
Historical Accuracy ◽  
Molecular Phylogenetic

The historical accuracy of building taxonomies is improved when they are based on phylogenetic inference (i.e., the resultant classifications are less apt to misrepresent evolutionary history). In fact, taxonomies inferred from statistically significant diagnostic morphological characters in the absence of phylogenetic considerations, can contain non-monophyletic lineages. This is especially true at the species level where small amounts of gene flow may not preclude the evolution of localized adaptions in different geographic areas while underpinning the paraphyletic nature of each population with respect to the other. We illustrate this point by examining genetic and morphological variation among three putatively allopatric populations of the granite-dwelling Bent-toed Gecko Cyrtodactylus aequalis from hilly regions in southeastern Myanmar. In the absence of molecular phylogenetic inference, a compelling argument for three morphologically diagnosable species could be marshaled. However, when basing the morphological analyses of geographic variation on a molecular phylogeny, there is a more compelling argument that only one species should be recognized. We are cognizant of the fact however, that when dealing with rare species or specimens for which no molecular data are possible, judicious morphological analyses are the only option—and the desired option given the current worldwide biodiversity crisis.

New combinations in Struthiopteris spicant for the European flora

Phytotaxa ◽  
2017 ◽  
Vol 302 (2) ◽  
pp. 198 ◽  
Author(s):  
PAWEL WASOWICZ ◽  
JOSE MARIA GABRIEL Y GALAN ◽  
RUBEN PINO PEREZ
Keyword(s):  
Morphological Characters ◽  
New Combinations ◽  
Phylogenetic Relation ◽  
Molecular Studies ◽  
Long Time

Delimitation of genera in Blechnaceae Newman (1844: 8), a subcosmopolitan fern family with ca. 250 species, has remained uncertain for a long time. During the last decade, evidence has been accumulating about the polyphyletism within Blechnum Linnaeus (1753: 1077) (e.g. Shepherd et al. 2007, Rothfels et al. 2012, Gabriel y Galán et al. 2013, Perrie et al. 2014). Recent molecular studies (Gasper et al. 2016a) lead to an updated classification attempting to put morphological characters into a natural, phylogenetic relation (Gasper et al. 2016b). Because of these changes, the species most people associate with the genus Blechnum, B. spicant (Linnaeus 1753: 1066) Roth (1794: 56), is now treated under Struthiopteris Scopoli (1754: 25).

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