Tracking single molecule dynamics in the adult Drosophila brain

UQ eSpace ◽  
2021 ◽  
Author(s):  
Adam D Hines ◽  
Bruno van Swinderen
Keyword(s):  
Single Molecule ◽  
Single Molecule Dynamics ◽  
Drosophila Brain ◽  
Molecule Dynamics ◽  
Adult Drosophila

scholarly journals Tracking single molecule dynamics in the adult Drosophila brain

eNeuro ◽  
2021 ◽  
pp. ENEURO.0057-21.2021
Author(s):  
Adam D. Hines ◽  
Bruno van Swinderen
Keyword(s):  
Single Molecule ◽  
Single Molecule Dynamics ◽  
Drosophila Brain ◽  
Molecule Dynamics ◽  
Adult Drosophila

scholarly journals Tracking single molecule dynamics in the anesthetized Drosophila brain

2020 ◽  
Author(s):  
Adam D. Hines ◽  
Bruno van Swinderen
Keyword(s):  
General Anesthesia ◽  
Single Molecule ◽  
Ex Vivo ◽  
Super Resolution ◽  
Valuable Insight ◽  
General Anesthetics ◽  
Novel Approach ◽  
Single Molecule Dynamics ◽  
Drosophila Brain ◽  
Molecule Dynamics

AbstractSuper-resolution microscopy provides valuable insight for understanding the nanoscale organization within living tissue, although this method is typically restricted to cultured or dissociated cells. Here, we develop a method to track the mobility of individual proteins in ex vivo adult Drosophila melanogaster brains, focusing on a key component of the presynaptic release machinery, syntaxin1A. We show that individual syntaxin1A dynamics can be reliably tracked within neurons in the whole fly brain, and that the mobility of syntaxin1A molecules increases following conditional neural stimulation. We then apply this preparation to the problem of general anesthesia, to address how different anesthetics might affect single molecule dynamics in intact brain synapses. We find that propofol, etomidate, and isoflurane significantly impair syntaxin1A mobility, while ketamine and sevoflurane have little effect. Resolving single molecule dynamics in intact fly brains provides a novel approach to link localized molecular effects with systems-level phenomena such as general anesthesia.Impact statementA new approach to track the mobility of individual molecules using intact fly brains reveals a common presynaptic effect for different intravenous and volatile general anesthetics.

Multidimensional Characterization of Single‐Molecule Dynamics in a Plasmonic Nanocavity

2021 ◽  
Author(s):  
Lucas Domulevicz ◽  
Hyunhak Jeong ◽  
Nayan K. Paul ◽  
Juan Sebastian Gomez-Diaz ◽  
Joshua Hihath
Keyword(s):  
Single Molecule ◽  
Single Molecule Dynamics ◽  
Molecule Dynamics

Single-molecule dynamics of site-specific labeled transforming growth factor type II receptors on living cells

2014 ◽  
Vol 50 (94) ◽  
pp. 14724-14727 ◽  
Author(s):  
Ming Cheng ◽  
Wei Zhang ◽  
Jinghe Yuan ◽  
Wangxi Luo ◽  
Nan Li ◽  
...  
Keyword(s):  
Growth Factor ◽  
Single Molecule ◽  
Transforming Growth Factor ◽  
Living Cells ◽  
Unnatural Amino Acid ◽  
Type Ii ◽  
Site Specific ◽  
Single Molecule Dynamics ◽  
Factor Type ◽  
Molecule Dynamics

Single-molecule dynamics of the transforming growth factor type II receptor (TβRII) labeled by an unnatural amino acid.

Nanoconfinement greatly speeds up the nucleation and the annealing in single-DNA collapse

Soft Matter ◽  
2017 ◽  
Vol 13 (37) ◽  
pp. 6363-6371 ◽  
Author(s):  
Liang Dai ◽  
Jeremy J. Jones ◽  
Alexander R. Klotz ◽  
Stephen Levy ◽  
Patrick S. Doyle
Keyword(s):  
Single Molecule ◽  
Single Molecule Dynamics ◽  
New Phenomena ◽  
Molecule Dynamics

Manipulating and measuring single-molecule dynamics and reactions in nanofluidics is a rapidly growing field with broad applications in developing new biotechnologies, understanding nanoconfinement effects in vivo, and exploring new phenomena in confinement.

scholarly journals InferenceMAP: mapping of single-molecule dynamics with Bayesian inference

2015 ◽  
Vol 12 (7) ◽  
pp. 594-595 ◽  
Author(s):  
Mohamed El Beheiry ◽  
Maxime Dahan ◽  
Jean-Baptiste Masson
Keyword(s):  
Bayesian Inference ◽  
Single Molecule ◽  
Single Molecule Dynamics ◽  
Molecule Dynamics

Variable Temperature Single-Molecule Dynamics of MEH-PPV

ChemPhysChem ◽  
2005 ◽  
Vol 6 (11) ◽  
pp. 2404-2409 ◽  
Author(s):  
Young Jong Lee ◽  
Doo Young Kim ◽  
John K. Grey ◽  
Paul F. Barbara
Keyword(s):  
Single Molecule ◽  
Variable Temperature ◽  
Single Molecule Dynamics ◽  
Molecule Dynamics

scholarly journals Biophysical nanotools for single-molecule dynamics

2018 ◽  
Vol 10 (5) ◽  
pp. 1349-1357 ◽  
Author(s):  
Qian Peter Su ◽  
Lining Arnold Ju
Keyword(s):  
Single Molecule ◽  
Single Molecule Dynamics ◽  
Molecule Dynamics
Sign in / Sign up

Export Citation Format

Share Document