scholarly journals Protective effect of ethyl acetate extract of Ishige okamurae against carbon tetrachloride-induced acute liver injury in rats

2011 ◽  
Vol 51 (4) ◽  
pp. 259-265
Author(s):  
Sohi Kang ◽  
Wonjun Yang ◽  
Hanseul Oh ◽  
Yeonji Bae ◽  
Meejung Ahn ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Protective Effect ◽  
Ethyl Acetate ◽  
Acute Liver Injury ◽  
Ethyl Acetate Extract ◽  
Ishige Okamurae

scholarly journals A Polyphenol-Rich Fraction from Eugenia uniflora Exhibits Antioxidant and Hepatoprotective Activities In Vivo

2020 ◽  
Vol 13 (5) ◽  
pp. 84
Author(s):  
Mansour Sobeh ◽  
Marwa S. Hamza ◽  
Mohamed L. Ashour ◽  
Mona Elkhatieb ◽  
Mohamed A El Raey ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Ethyl Acetate ◽  
Acute Liver Injury ◽  
Antioxidant Activities ◽  
Biological Effects ◽  
Ethyl Acetate Fraction ◽  
Eugenia Uniflora ◽  
Quercetin Derivatives

Leaves from Eugenia uniflora, the red Brazilian cherry, have a high content of flavonoids that possess several biological effects such as anti-inflammatory, antioxidant, and antidiabetic activities. However, their influence on carbon tetrachloride (CCl4)-induced acute liver injury in rats has not been investigated. In the current study, a bioguided fractionation assay revealed that the ethyl acetate fraction (EAF) of Eugenia uniflora is the safest and most active fraction. LC-MS analysis of the ethyl acetate fraction revealed 22 secondary metabolites, mainly myricetin and quercetin derivatives. EAF did not show toxicity up to 2000 mg/kg, and exhibited antioxidant activities in vitro in DPPH assay with IC50 of 3.35 µg/mL. Additionally, EAF exhibited substantial antioxidant activities in vivo by counteracting the oxidative damage of the prooxidant juglone [80 µM] in Caenorhabditis elegans model organism and increased its survival rate in a dose-dependent fashion through the DAF-16/Foxo pathway. Furthermore, the hepatoprotective activity of EAF (200 mg/kg against carbon tetrachloride (CCl4) intoxicated male Wistar rats was assessed. EAF significantly inhibited CCl4-induced elevation of alanine aminotransferase (ALT), aspartate transaminase (AST), total bilirubin (TB), total cholesterol (TC), and triglycerides (TG), in the blood serum and prevented lipid peroxidation and restored superoxide dismutase (SOD) activity and glutathione (GSH) content in liver tissues. The observed hepatoprotective effects of EAF, which were supported by histopathological observations as pretreatment with EAF, effectively attenuated the CCl4-induced histopathological changes. In conclusion, EAF of Eugenia uniflora leaves has substantial hepatoprotective activities against CCl4 induced acute liver injury in rats due to its antioxidant activity.

scholarly journals Protective Effect of Eckol against Acute Hepatic Injury Induced by Carbon Tetrachloride in Mice

Marine Drugs ◽  
2018 ◽  
Vol 16 (9) ◽  
pp. 300 ◽  
Author(s):  
Shulan Li ◽  
Juan Liu ◽  
Mengya Zhang ◽  
Yuan Chen ◽  
Tianxing Zhu ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Protective Effect ◽  
Acute Liver Injury ◽  
Treated Group ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Enhanced Expression ◽  
Pre Treatment

Several in vitro studies have shown the potential hepatoprotective properties of eckol, a natural phlorotannin derived from the brown alga. However, the in vivo hepatoprotective potential of eckol has not been determined. In this study, we performed an in vivo study to investigate the protective effect of eckol and its possible mechanisms on the carbon tetrachloride (CCl4)-induced acute liver injury model in mice. Results revealed that eckol pre-treatment at the dose of 0.5 and 1.0 mg/kg/day for 7 days significantly suppressed the CCl4-induced increases of alanine transaminase (ALT) and aspartate aminotransferase (AST) levels in serum and meliorated morphological liver injury. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) analysis showed that the number of positive apoptotic hepatocytes in the eckol-treated group was lower than that in the CCl4 model group. Western blotting analysis also demonstrated the enhanced expression of bcl-2 and suppressed expression of cleaved caspase-3 by eckol. The CCl4-induced oxidative stress in liver was significantly ameliorated by eckol, which was characterized by reduced malondialdehyde (MDA) formations, and enhanced superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and glutathione (GSH) content. Moreover, the CCl4-induced elevations of pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 were markedly suppressed in the eckol-treated group. However, eckol enhanced the level of IL-10, a potent anti-inflammatory cytokine, and recruited CD11c+ dendritic cells into the liver tissues of CCl4-treated mice. These results indicated that eckol has the protective effect on CCl4-induced acute liver injury via multiple mechanisms including anti-apoptosis, anti-oxidation, anti-inflammation and immune regulation.

scholarly journals Protective effect and mechanism of ginsenoside Rg1 on carbon tetrachloride-induced acute liver injury

2017 ◽  
Vol 16 (3) ◽  
pp. 2814-2822 ◽  
Author(s):  
Benquan Qi ◽  
Suzhi Zhang ◽  
Daohua Guo ◽  
Sanxing Guo ◽  
Xiaodong Jiang ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Protective Effect ◽  
Acute Liver Injury ◽  
Ginsenoside Rg1

scholarly journals Protective Effect of Rifampicin against Acute Liver Injury Induced by Carbon Tetrachloride in Mice

1995 ◽  
Vol 69 (4) ◽  
pp. 325-334 ◽  
Author(s):  
Renbin Huang ◽  
Hiroyasu Okuno ◽  
Masashi Takasu ◽  
Yasuko Shiozaki ◽  
Kyoichi Inoue
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Protective Effect ◽  
Acute Liver Injury

Protective effect of antrosterol from Antrodia camphorata submerged whole broth against carbon tetrachloride-induced acute liver injury in mice

2012 ◽  
Vol 132 (2) ◽  
pp. 709-716 ◽  
Author(s):  
Guan-Jhong Huang ◽  
Jeng-Shyan Deng ◽  
Shyh-Shyun Huang ◽  
Yi-Yuan Shao ◽  
Chin-Chu Chen ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Protective Effect ◽  
Acute Liver Injury ◽  
Antrodia Camphorata

Mechanism of the Protective Effects of Sumac Gall Extract and Gallic Acid on the Progression of CCl4-induced Acute Liver Injury in Rats

1998 ◽  
Vol 26 (03n04) ◽  
pp. 333-341 ◽  
Author(s):  
Shigeyuki Kanai ◽  
Hideyuki Okano
Keyword(s):  
Superoxide Dismutase ◽  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Protective Effect ◽  
Gallic Acid ◽  
Cell Membranes ◽  
Acute Liver Injury ◽  
Radical Scavenging ◽  
Protective Effects ◽  
Preventive Effect

To examine the mechanism of the preventive effect of tannins on the progression of carbon tetrachloride (CCl4)-induced acute liver injury in rats, sumac gall (SG) extract and gallic acid (GA) were used as substitutes for crude tannins, because SG is a kind of Chinese traditional medicinal herb containing large amounts of various tannins, and GA is one of the major constituents of SG. The protective effect of oral (p.o.) and intraperitoneal (i.p.) administration of each substance on progression of CCl4-induced hepatitis was investigated in rats. Speculating that the superoxide dismutase (SOD)-like activities (O2 radical-scavenging activities) and/or protective effects of these substances on cell membranes might play a key role in the mechanism opposing the progression of CCl4-induced hepatitis, the O2 radical-scavenging activities in liver cells and serum in rats were monitored. Both substances significantly prevented the progression of acute liver injury with both p.o. and i.p. administration. These findings suggest that the mechanism for this prevention might be due mainly to the protective effect of these substances on cell membranes rather than O2 radical-scavenging activities.

scholarly journals Antioxidant and Protective Effect of Ethyl Acetate Extract ofPodophyllum hexandrumRhizome on Carbon Tetrachloride Induced Rat Liver Injury

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Showkat Ahmad Ganie ◽  
Ehtishamul Haq ◽  
Akbar Masood ◽  
Abid Hamid ◽  
Mohmmad Afzal Zargar
Keyword(s):  
Carbon Tetrachloride ◽  
Protective Effect ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Peroxide Radical ◽  
Scavenging Activity

The antioxidant and hepatoprotective activities of ethyl acetate extract was carefully investigated by the methods of DPPH radical scavenging activity, Hydroxyl radical scavenging activity, superoxide radical scavenging activity, hydrogen peroxide radical scavenging activity, and its reducing power ability. All thesein vitroantioxidant activities were concentration dependent, which were compared with standard antioxidants such as BHT, α-tocopherol. The hepatoprotective potential ofPodophyllum hexandrumextract was also evaluated in male Wistar rats against carbon tetrachloride- (CCl4-) induced liver damage. Pretreated rats were given ethyl acetate extract at 20, 30, and 50 mg/kg dose prior to CCl4administration (1 mL/kg, 1:1 in olive oil). Rats pretreated withP. hexandrumextract remarkably prevented the elevation of serum AST, ALT, LDH, and liver lipid peroxides in CCl4-treated rats. Hepatic glutathione levels were significantly increased by the treatment with the extract in all the experimental groups. The extract at the tested doses also restored the levels of liver homogenate enzymes (glutathione peroxidase, glutathione reductase, superoxide dismutase, and glutathione-S-transferase) significantly. This study suggests that ethyl acetate extract ofP. hexandrumhas a liver-protective effect against CCl4-induced hepatotoxicity and possessin vitroantioxidant activities.

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