scholarly journals Detección molecular y análisis filogenético de Hepatozoon canis (Eucoccidiorida: Haemogregarinidae) en perros clínicamente sanos de Bahía Blanca (Buenos Aires)

2017 ◽  
Vol 16 (1) ◽  
pp. 46-49
Author(s):  
Gabriel CICUTTIN ◽  
María N. DE SALVO
Keyword(s):  
Buenos Aires ◽  
Hepatozoon Canis ◽  
San Luis ◽  
Babesia Spp ◽  
Theileria Spp

Las enfermedades caninas causadas por protozoarios y transmitidas por garrapatas representan un importante problema en medicina veterinaria. El objetivo del estudio fue detectar molecularmente Hepatozoon spp., Babesia spp. y Theileria spp. en perros clínicamente sanos de distintas regiones de Argentina y analizar la diversidad genética de los hallazgos obtenidos. Se analizaron 163 muestras de ADN de sangre de perros (40 de Ciudad Autónoma de Buenos Aires; 33 de Bahía Blanca, Buenos Aires; 15 de Castelli, Chaco; 27 de Salsipuedes, Córdoba; 40 de Merlo, San Luis; y 8 de San Miguel, Corrientes). Mediante una PCR que amplifica un fragmento variable (460-540 pb) del gen ARNr 18S incluyendo la región V4 de los géneros Hepatozoon, Babesia y Theileria, el 12,1 % (4/33) de los perros de Bahía Blanca (Buenos Aires) resultaron positivos. Las secuencias obtenidas se identificaron como Hepatozoon canis y resultaron filogenéticamente similares a hallazgos en Sudamérica y en el resto del mundo. El estudio de H. canis en Argentina mediante técnicas moleculares de diagnóstico junto con el análisis filogenético resulta de suma importancia para conocer la situación de este patógeno en el país.

scholarly journals Ticks and associated pathogens in camels (Camelus dromedarius) from Riyadh province, Saudi Arabia

2020 ◽  
Author(s):  
Abdullah Daria Alanazi ◽  
Viet Linh Nguyen ◽  
Mohamed Saleh Alyousif ◽  
Ranju Ravindran Santhakumari Manoj ◽  
Abdulaziz Saleh Alouffi ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Anaplasma Phagocytophilum ◽  
Ixodid Ticks ◽  
Hepatozoon Canis ◽  
Hyalomma Dromedarii ◽  
Health And Wellbeing ◽  
Hyalomma Excavatum ◽  
Prevalent Species ◽  
Babesia Spp ◽  
Theileria Spp

Abstract Background: Camel production in Saudi Arabia is severely affected by various diseases and by inadequate veterinary services. Ticks and tick-borne pathogens (TBPs ) affect the health and wellbeing of camels consequently diminishing their productivity and performances. In addition, camels may act as hosts for TBPs (e.g. Anaplasma phagocytophilum ) causing diseases in humans. The current study aimed to determine the prevalence of ixodid ticks and molecularly investigate the associated pathogens in camels from Saudi Arabia. Methods: Blood and tick samples were collected from camels ( n = 170) in Riyad Province of Saudi Arabia. Ticks were morphologically identified, and blood of camels were molecularly screened for apicomplexan (i.e. Babesia spp . , Theileria spp., Hepatozoon spp.) and rickettsial parasites (i.e. Ehrlichia spp. and Anaplasma spp.). Results: Of the 170 camels examined, 116 (68.2%; 95% CI: 60.9–75.1%) were infested by ticks with a mean intensity of 2.53 (95% CI: 2.4–2.6). In total of 296 ticks collected, Hyalomma dromedarii was the most prevalent (76.4%), followed by Hyalomma impeltatum (23.3%) and Hyalomma excavatum (0.3%). Of the tested animals, 13 (7.6%; 95% CI: 4.3–12.8%) scored positive to at least one TBP, with Anaplasma platys (5.3%; 95% CI: 2.7–9.9%) being the most prevalent species, followed by Anaplasma phagocytophilum , Anaplasma sp., Ehrlichia canis and Hepatozoon canis (0.6% each; 95% CI: 0.04–3.4%). None of the camels were found to be co-infected with more than one pathogen. All samples tested negative for Babesia spp. and Theileria spp. Conclusions: The present study reveals the occurrence of different tick species and tick-borne pathogens in camels from Saudi Arabia. Importantly, these camels may carry A. phagocytophilum and A. platys , representing a potential risk to humans.

scholarly journals Ticks and associated pathogens in camels (Camelus dromedarius) from Riyadh province, Saudi Arabia

2020 ◽  
Author(s):  
Abdullah Daria Alanazi ◽  
Viet Linh Nguyen ◽  
Mohamed Saleh Alyousif ◽  
Ranju Ravindran Santhakumari Manoj ◽  
Abdulaziz Saleh Alouffi ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Anaplasma Phagocytophilum ◽  
Ixodid Ticks ◽  
Hepatozoon Canis ◽  
Veterinary Services ◽  
Hyalomma Dromedarii ◽  
Hyalomma Excavatum ◽  
Prevalent Species ◽  
Babesia Spp ◽  
Theileria Spp

Abstract Background Camel production in Saudi Arabia is severely affected by various diseases and by inadequate veterinary services. Ticks and tick-borne pathogens (TBPs) represent a major threat to the health of camels consequently affecting their productivity and performances. In addition, camels may act as hosts for TBPs (e.g. Anaplasma phagocytophilum ) causing diseases in humans. The current study aimed to determine the prevalence of ixodid ticks and molecularly investigate the associated pathogens in camels from Saudi Arabia. Methods Blood and tick samples were collected from camels (n=170) in Riyad Province of Saudi Arabia. Ticks were morphologically identified and blood of camels were molecularly screened for apicomplexan (i.e. Babesia spp ., Theileria spp., Hepatozoon spp.) and rickettsial parasites (i.e. Ehrlichia spp. and Anaplasma spp.). Results Of the 170 camels examined, 116 (68.2%; 95% CI: 60.9–75.1) were infested by ticks with a mean intensity of 2.53 (95% CI =2.4–2.6). In total of 296 ticks collected, Hyalomma dromedarii was the most prevalent (76.4%), followed by Hyalomma impeltatum (23.3%) and Hyalomma excavatum (0.3%). Of the tested animals, 13 (7.6%; 95% CI: 4.3–12.8) scored positive to at least one TBP, with Anaplasma platys (5.3%; 95% CI: 2.7–9.9) being the most prevalent species, followed by Anaplasma phagocytophilum , Anaplasma sp., Ehrlichia canis and Hepatozoon canis (0.6% each; 95% CI: 0.04–3.4). None of the camels were found to be co-infected with more than one pathogen. All samples tested negative for Babesia spp. and Theileria spp. Conclusions Current study reveals the occurrence of different tick species and tick-borne pathogens in camels from Saudi Arabia. Importantly, these camels may carry A. phagocytophilum and A. platys , representing a potential risk to humans.

scholarly journals Ticks and associated pathogens in camels (Camelus dromedarius) from Riyadh province, Saudi Arabia

2019 ◽  
Author(s):  
Abdullah Daria Alanazi ◽  
Viet Linh Nguyen ◽  
Mohamed Saleh Alyousif ◽  
Ranju Ravindran Santhakumari Manoj ◽  
Abdulaziz Saleh Alouffi ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Anaplasma Phagocytophilum ◽  
Ixodid Ticks ◽  
Hepatozoon Canis ◽  
Veterinary Services ◽  
Hyalomma Dromedarii ◽  
Hyalomma Excavatum ◽  
Prevalent Species ◽  
Babesia Spp ◽  
Theileria Spp

Abstract Background Camel production in Saudi Arabia is severely affected by various diseases and by inadequate veterinary services. Ticks and tick-borne pathogens (TBPs) represent a major threat to the health of camels consequently affecting their productivity and performances. In addition, camels may act as hosts for TBPs (e.g. Anaplasma phagocytophilum ) causing diseases in humans. The current study aimed to determine the prevalence of ixodid ticks and molecularly investigate the associated pathogens in camels from Saudi Arabia.Methods Blood and tick samples were collected from camels (n=170) in Riyad Province of Saudi Arabia. Ticks were morphologically identified and blood of camels were molecularly screened for apicomplexan (i.e. Babesia spp ., Theileria spp., Hepatozoon spp.) and rickettsial parasites (i.e. Ehrlichia spp. and Anaplasma spp.).Results Of the 170 camels examined, 116 (68.2%; 95% CI: 60.9–75.1) were infested by ticks with a mean intensity of 2.53 (95% CI =2.4–2.6). In total of 296 ticks collected, Hyalomma dromedarii was the most prevalent (76.4%), followed by Hyalomma impeltatum (23.3%) and Hyalomma excavatum (0.3%). Of the tested animals, 13 (7.6%; 95% CI: 4.3–12.8) scored positive to at least one TBP, with Anaplasma platys (5.3%; 95% CI: 2.7–9.9) being the most prevalent species, followed by Anaplasma phagocytophilum , Anaplasma sp., Ehrlichia canis and Hepatozoon canis (0.6% each; 95% CI: 0.04–3.4). None of the camels were found to be co-infected with more than one pathogen. All samples tested negative for Babesia spp. and Theileria spp.Conclusions Current study reveals the occurrence of different tick species and tick-borne pathogens in camels from Saudi Arabia. Importantly, these camels may carry A. phagocytophilum and A. platys , representing a potential risk to humans.

scholarly journals ¿Qué sabemos y qué nos falta estudiar sobre agentes infecciosos transmitidos por garrapatas en Costa Rica?

2018 ◽  
Vol 36 (3) ◽  
pp. 48
Author(s):  
Gaby Dolz ◽  
Alexander Barrantes González ◽  
Marta C. Bonilla ◽  
Liliana Campos ◽  
Laura Bouza Mora ◽  
...  
Keyword(s):  
Costa Rica ◽  
Ehrlichia Canis ◽  
Hepatozoon Canis ◽  
De Medicina ◽  
Babesia Spp ◽  
Theileria Spp

Las condiciones propias de un país tropical como Costa Rica representan grandes ventajas para ladistribución y actividad de las garrapatas, así como facilita que exista una serie de hospedadores paraeste tipo de ectoparásitos. Ambos factores inciden en la presentación y prevalencia de enfermedadesvectoriales zoonóticas. En el presente trabajo se presentan los principales resultados obtenidos en lasinvestigaciones realizadas durante los últimos diez años en la Escuela de Medicina Veterinaria, en lascuales se ha determinado la presencia, se ha estimado la prevalencia y se ha establecido la importancia,en la salud pública, de agentes rickettsiales (Ehrlichia spp., Anaplasma spp. y Rickettsia spp.), piroplasmas(Babesia spp., Theileria spp.), y Hepatozoon canis en animales domésticos (caninos, felinos, equinosy ovinos), animales silvestres (mapaches, pizotes, venados cola blanca) y garrapatas de nuestro país.Además, se presentan los resultados obtenidos en un estudio serológico de donadores de sangre; enespecial, la identificación de un nuevo genotipo de Ehrlichia canis hallado en ellos. Finalmente, sediscute sobre la diseminación, transmisión y curso de las diferentes enfermedades, como también sobreel diagnóstico, el tratamiento y los estudios que hacen falta para establecer las medidas de controlapropiadas a adoptar para el control de estas enfermedades en nuestro país.

scholarly journals Blood survey of Babesia spp and Theileria spp in Monos cattle, Benin

2016 ◽  
Vol 11 (14) ◽  
pp. 1266-1272 ◽  
Author(s):  
B Adehan Safiou ◽  
Biguezoton Abel ◽  
Dossoumou Aquilas ◽  
N Assogba Marc ◽  
Adehan Razacki ◽  
...  
Keyword(s):  
Babesia Spp ◽  
Theileria Spp

scholarly journals Celia Lapalma de Emery: experiencia en la cooperación salesiana. Argentina, 1906-1929

2021 ◽  
Vol 29 (2) ◽  
Author(s):  
Lucía Bracamonte
Keyword(s):  
Buenos Aires ◽  
San Luis ◽  
Las Mujeres

Resumen: Celia Lapalma, quien nació en 1867 en Salto (provincia de San Luis) y falleció en 1937 en Buenos Aires, fue una militante católica reconocida por su compromiso con la situación de las obreras y la infancia desvalida. Además de integrar asociaciones benéficas y desempeñar funciones de inspección en organismos oficiales fue docente, escritora, conferencista, esposa y madre. En este trabajo se analiza su actuación como tesorera de la Comisión Central de Señoras Cooperadoras Salesianas Argentinas, con el objetivo de identificar las articulaciones que realizaba en sus discursos epistolares entre su función en la congregación y sus papeles laborales y familiares. Esto permitirá aportar, a partir de su itinerario y sus representaciones de género, a la comprensión de la experiencia como benefactoras de las mujeres de la elite, en la esfera de la sociabilidad asociativa

Molecular Characterization and Phylogenetic Analysis of Theileria spp. and Babesia spp. Isolated from Various Ticks in Southeastern and Northwestern Regions of Iran

2018 ◽  
Vol 18 (11) ◽  
pp. 595-600 ◽  
Author(s):  
Ahmad Jafarbekloo ◽  
Maryam Roya Ramzgouyan ◽  
Sadegh Shirian ◽  
Leila Tajedin ◽  
Hassan Bakhshi ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Babesia Spp ◽  
Theileria Spp

scholarly journals Occurrence of Ehrlichia canis and Hepatozoon canis and probable exposure to Rickettsia amblyommatis in dogs and cats in Natal, RN

2019 ◽  
Vol 28 (1) ◽  
pp. 151-156 ◽  
Author(s):  
Marcos Gomes Lopes ◽  
Felipe da Silva Krawczak ◽  
Julia Teresa Ribeiro de Lima ◽  
Gislene Fatima da Silva Rocha Fournier ◽  
Igor da Cunha Lima Acosta ◽  
...  
Keyword(s):  
Spotted Fever ◽  
Immunofluorescence Assay ◽  
Ehrlichia Canis ◽  
Hepatozoon Canis ◽  
Spotted Fever Group ◽  
Accession Number ◽  
Serum Samples ◽  
Control Center ◽  
Related Genotype ◽  
Babesia Spp

Abstract Occurrence of infection or exposure to Ehrlichia canis, Hepatozoon canis and Rickettsia spp. was detected in feral cats living in two fragments from Atlantic rainforest, in Natal, RN, Brazil, and in dogs living around the parks. While serum samples were collected from 155 animals (53 cats living in the parks; 29 dogs living in human homes around the parks; and 73 dogs living at an animal control center - ACC), spleen samples were collected from 20 dogs that were euthanized at ACC. Serum samples were analyzed to Rickettsia spp. and E. canis antibodies using the indirect immunofluorescence assay. Seventeen of the 102 dogs (17%) had E. canis antibodies and 13% (20/155) of all dogs and cats (i.e. 3% (3/102) of the dogs and 32% (17/53) of the cats) were seropositive for Rickettsia spp. antigens. The animals were therefore been exposed to R. amblyommatis or by a very closely related genotype. Among the 20 dog spleen samples analyzed, eight were PCR positive for E. canis and two for H. canis (GenBank accession number MG772657 and MG772658, respectively). In none of the spleen samples were obtained amplicons for Babesia spp. through PCR. This study provided the first evidence that Rickettsia of the spotted fever group is circulating among dogs and cats in Natal.

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