scholarly journals Technical validation of a polymerase chain reaction for Chlamydia trachomatis detection

2016 ◽  
Vol 32 (3) ◽  
pp. 398-410
Author(s):  
Diana Jurado-Orejuela ◽  
◽  
Claudia Paredes-Amaya ◽  
Gerardo Libreros-Zúñiga ◽  
◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Chain Reaction ◽  
Technical Validation ◽  
Polymerase Chain

scholarly journals Technical validation of a polymerase chain reaction for Chlamydia trachomatis detection

2021 ◽  
Vol 32 (3) ◽  
pp. 398-410
Author(s):  
Diana Jurado-Orejuela ◽  
Claudia Paredes-Amaya ◽  
Gerardo Libreros-Zúñiga
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Chain Reaction ◽  
Technical Validation ◽  
Polymerase Chain ◽  
Para Determinar

Objetivo: Implementar una técnica de PCR (Reacción en cadena de la polimerasa) para la detección de Chlamydia trachomatis. Materiales y Métodos: Se realizó un estudio experimental en el que se estandarizaron las condiciones de PCR (Concentración de MgCl2 , Taq polimerasa y temperatura de alineamiento de cebadores) para la amplificación de una región de 201pb del plásmido de C. trachomatis con los cebadores CtP1 5´-TAGTAACTGCCACTTCATCA-3´ y CtP2 5´- TTCCCCTTGTAATTCGTTGC-3. Se realizaron diluciones seriadas del ADN de C. trachomatis ATCC VR885D para determinar la sensibilidad analítica. La especificidad analítica de los cebadores se determinó con ADN de diferentes microorganismos patógenos y comensales del tracto urogenital. Se determinó la variabilidad intra e interensayo de la PCR sobre triplicados de diferentes muestras de ADN. Resultados: Las condiciones de amplificación fueron 94°C/4 min, seguido de 40 ciclos a 94°C/1 min, 56°C/1 min y 72°C/1.5 min y extensión final de 72°C/4 min. La concentración óptima de MgCl2 fue 1.5 mM y de ADN polimerasa 1U. La sensibilidad analítica de la prueba fue 4.8 x10-15g/mL de ADN equivalentes a 160 cuerpos elementales de C. trachomatis. La especificidad analítica fue 100% y la variabilidad intra e interensayo mostraron reproducibilidad de la PCR. Conclusiones: Los datos sugieren que esta PCR puede emplearse con fines diagnósticos. Se requieren estudios adicionales para la evaluación clínica de esta prueba.

scholarly journals Chlamydia trachomatis detection in HIV infected patients using polymerase chain reaction

2013 ◽  
Vol 2 (1) ◽  
pp. 12-16
Author(s):  
A Shrestha ◽  
N Adhikari ◽  
Y Shah ◽  
P Poudel ◽  
B Acharya ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Public Health Problem ◽  
Chlamydia Infection ◽  
Hiv Positive ◽  
Chain Reaction ◽  
Hiv Patients ◽  
Important Public Health Problem ◽  
Sexually Transmitted ◽  
Polymerase Chain

Introduction: Chlamydia trachomatis is a sexually transmitted organism and causes important public health problem in the sexually active age group. Limited studies are found regarding the prevalence of C. trachomatis in Nepal. Moreover, currently there are no any study in Nepal reporting the association of chlamydia and HIV infection. This study attempts to determine the burden of chlamydia on HIV positive patients. Materials and Methods: A total of 117 HIV positive patients visiting a HIV clinic in Kathmandu, were screened for chlamydia infection. For this, urine samples were collected and analyzed using the Polymerase Chain Reaction Technique (PCR). Results: C. trachomatis was detected in 4.2% of the total 117 HIV patients. Out of positive cases 60% were males and 40% were females. However, chlamydia was found more prevalent among females (6.8%) than males (3.4%). Eighty percent of positive cases were asymptomatic. Conclusions: Although, the prevalence of chlamydia infection was found less HIV patients, most of those cases were asymptomatic. Therefore, routine checkup is recommended for all suspected cases for timely management of the disease. DOI: http://doi.dx.org/10.3126/ijim.v2i1.8003 Int J Infect Microbiol 2013;2(1):12-16

scholarly journals Evaluation of Clearview and Magic Lite tests, polymerase chain reaction, and cell culture for detection of Chlamydia trachomatis in urogenital specimens.

1993 ◽  
Vol 31 (12) ◽  
pp. 3204-3210 ◽  
Author(s):  
J A Kluytmans ◽  
W H Goessens ◽  
J W Mouton ◽  
J H van Rijsoort-Vos ◽  
H G Niesters ◽  
...  
Keyword(s):  
Cell Culture ◽  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Chain Reaction ◽  
Polymerase Chain

Development and clinical evaluation of a polymerase chain reaction test for detection of Chlamydia trachomatis.

1992 ◽  
Vol 30 (8) ◽  
pp. 2122-2128 ◽  
Author(s):  
J M Ossewaarde ◽  
M Rieffe ◽  
M Rozenberg-Arska ◽  
P M Ossenkoppele ◽  
R P Nawrocki ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Clinical Evaluation ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Chain Reaction Test

scholarly journals Prevalence of Chlamydia trachomatis infection diagnosed by polymerase chain reaction in female sex workers in a Northern Mexican City

2019 ◽  
Vol 9 (1) ◽  
pp. 5-8
Author(s):  
Luis Francisco Sánchez-Anguiano ◽  
Nadia Velázquez-Hernández ◽  
Fernando Martín Guerra-Infante ◽  
Marisela Aguilar-Durán ◽  
Alma Rosa Pérez-Álamos ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Sex Workers ◽  
Female Sex Workers ◽  
Chain Reaction ◽  
Chlamydia Trachomatis Infection ◽  
Female Sex ◽  
Polymerase Chain
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