scholarly journals Direct shoot regeneration from male immature flower buds of Musa paradisiaca Linn. cv. Poovan (AAB)

2018 ◽  
Vol 5 (4) ◽  
pp. 142-148
Author(s):  
Anjana R G Nair ◽  
P Ravichandran ◽  
Mathew Bejoy
Keyword(s):  
Shoot Regeneration ◽  
Male Flower ◽  
Basal Region ◽  
Ms Medium ◽  
Direct Shoot Regeneration ◽  
Benzyl Adenine ◽  
Flower Buds ◽  
Flower Bud ◽  
Musa Paradisiaca ◽  
Direct Shoot

A tissue culture system has been developed to multiply Musa paradisiaca cv. Poovan using male immature flower bud and to establish it in ex vitro condition. Size of explants has been found an influencing factor for culture initiation. Immature male flower bud segments of 3 cm size were ideal for better survival and subsequent shoot regeneration. Direct shoot regeneration was achieved from male immature flower buds on Murashige and Skoog (MS) medium supplemented with varying concentrations of plant growth regulators. Initially, actively dividing meristematic region developed at the basal region of flower buds near the bract axil, which later grew into green shoot buds in most of the PGR treatments. Single use of benzyl adenine were found beneficial than kinetin or addition of indole-3-acetic acid. Maximum production of 31.0 ± 0.65 shoots was achieved on MS + 3% sucrose + 6 mg/L benzyl adenine in 15 weeks. Isolated healthy shoots were rooted in half-strength MS medium with 150 mg/L activated charcoal + 30 g/L sucrose + 1 mg/L indole-3-butyric acid within 15 days and they established successfully in greenhouse conditions with 85 % survival.

Direct shoot regeneration from epicotyl explants of grasspea (Lathyrus sativus)

2006 ◽  
Vol 54 (5) ◽  
pp. 505 ◽  
Author(s):  
Durga Prasad Barik ◽  
Umaballava Mohapatra ◽  
Pradeep Kumar Chand
Keyword(s):  
Shoot Regeneration ◽  
Adventitious Shoot ◽  
Lathyrus Sativus ◽  
Grain Legume ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Direct Shoot Regeneration ◽  
Murashige Skoog ◽  
Seedling Explant ◽  
Direct Shoot

A reproducible procedure is described for adventitious shoot organogenesis in epicotyl segments resulting in prolific plant regeneration of a grain legume grasspea (Lathyrus sativus L.). Among seedling explant types examined, epicotyl segments were most responsive. The highest percentage of direct shoot regeneration was elicited on Murashige–Skoog (MS) medium augmented with 4.0 mg L–1 6-benzyladenine (BA) + 2.0 mg L–1 α-naphthaleneacetic acid (NAA). Compared with four other genotypes examined, IC-120487 showed the highest shoot regeneration frequency (approximately 80%) with maximum shoot numbers (averaging eight shoots per explant) and longest average shoot length (approximately 4 cm). Rhizogenesis was induced in ~78% of the regenerated shoots in half-strength MS medium containing 0.5 mg L–1 indole-3-acetic acid (IAA). Plantlets were acclimated in vermi-compost and 75% of those transferred to soil survived and set viable seeds.

Direct shoot regeneration from immature inflorescence cultures of Chlorophytum arundinaceum and Chlorophytum borivilianum

Biologia ◽  
2009 ◽  
Vol 64 (2) ◽  
Author(s):  
Sanghamitra Samantaray ◽  
Saroj Kumar ◽  
Satyabrata Maiti
Keyword(s):  
Shoot Regeneration ◽  
Terminal Segment ◽  
Type I ◽  
Ms Medium ◽  
Direct Shoot Regeneration ◽  
Immature Inflorescence ◽  
Shoot Buds ◽  
Shoot Bud ◽  
Half Strength ◽  
Direct Shoot

AbstractDirect shoot regeneration was achieved from immature inflorescence explants of Chlorophytum arundinaceum and C. borivilianum on half-strength Murashige & Skoog (MS) medium supplemented with 3.0 mg L−1 BA, 150 mg L−1 Ads, 0.1 mg L−1 NAA and 3% (w/v) sucrose under a 16-h photoperiod. The shoot buds developed within 2–3 weeks of culture. High frequency of shoot bud regeneration was achieved when cultured on similar medium in subsequent subcultures. The apex portion (Type I) of the inflorescence produced more shoot buds as compared to the middle ones (type II). More than 75% of the terminal segment explants produced shoot buds within 4-week of culture. Response of basal portion (Type III) was negative for shoot bud initiation. Shoots rooted on half-strength basal MS medium supplemented with half-strength MS medium, 0.1 mg L−1 IAA and 2% (w/v) sucrose. Micropropagated plantlets were hardened in the green house and successfully established in the soil where 90% of the plants survived. This protocol would be useful for commercial micropropagation and genetic improvement prograrmme.

Direct shoot regeneration from leaf explants of Rosa damascena Mill.

2004 ◽  
Vol 40 (2) ◽  
pp. 192-195 ◽  
Author(s):  
Pratap Kumar Pati ◽  
Madhu Sharma ◽  
Anil Sood ◽  
P. S. Ahuja
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Rosa Damascena ◽  
Direct Shoot Regeneration ◽  
Direct Shoot

scholarly journals Improvement of micropropagation protocol of Phalaenopsis sp. for the method of direct shoot regeneration from nodes of floral spikes

2012 ◽  
pp. 141-150
Author(s):  
Marija Markovic ◽  
Milos Tanasic ◽  
Nevena Stojic ◽  
Radivoje Bulatovic ◽  
Marta Jovic ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Coconut Water ◽  
Soy Flour ◽  
Multiplication Rate ◽  
Direct Shoot Regeneration ◽  
Medium Components ◽  
Cultivation In Vitro ◽  
Direct Shoot

This paper succesfully investigated the possibility of modification of the micropropagation protocol of Phalaenopsis sp. with an aim to simplify the procedure and reduce the costs. The obtained results show that some medium components can be succesfully omitted (coconut water, glutamine, 2-morpholinoethanesulfonic acid) and some of them (peptone) can be replaced with a cheaper constituent (soy flour) while preserving the quality of the obtained microplants. The multiplication rate was 7,6 shoots per explant after the period of 150 days of cultivation in vitro. On the same medium 60% of explants were rooted and roots were mostly well developed.

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