scholarly journals Dysregulation of junctional adhesion molecule-A contributes to ethanol-induced barrier disruption in intestinal epithelial cell monolayers

2017 ◽  
Vol 5 (23) ◽  
pp. e13541 ◽  
Author(s):  
Daniel M. Chopyk ◽  
Pradeep Kumar ◽  
Reben Raeman ◽  
Yunshan Liu ◽  
Tekla Smith ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Adhesion Molecule ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Cell Monolayers ◽  
Barrier Disruption ◽  
Epithelial Cell Monolayers

scholarly journals A Novel Pharmacological Approach to Enhance the Integrity and Accelerate Restitution of the Intestinal Epithelial Barrier

2020 ◽  
Vol 26 (9) ◽  
pp. 1340-1352
Author(s):  
Xuelei Cao ◽  
Lei Sun ◽  
Susana Lechuga ◽  
Nayden G Naydenov ◽  
Alex Feygin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Intestinal Epithelial Barrier ◽  
Cell Monolayers ◽  
Barrier Disruption ◽  
Epithelial Cell Monolayers ◽  
Immunofluorescence Labeling ◽  
E Cadherin

Abstract Background Disruption of the gut barrier is an essential mechanism of inflammatory bowel diseases (IBDs) contributing to the development of mucosal inflammation. A hallmark of barrier disruption is the disassembly of epithelial adherens junctions (AJs) driven by decreased expression of a major AJ protein, E-cadherin. A group of isoxazole compounds, such as E-cadherin-upregulator (ECU) and ML327, were previously shown to stimulate E-cadherin expression in poorly differentiated human cancer cells. This study was designed to examine whether these isoxazole compounds can enhance and protect model intestinal epithelial barriers in vitro. Methods The study was conducted using T84, SK-CO15, and HT-29 human colonic epithelial cell monolayers. Disruption of the epithelial barrier was induced by pro-inflammatory cytokines, tumor necrosis factor-α, and interferon-γ. Barrier integrity and epithelial junction assembly was examined using different permeability assays, immunofluorescence labeling, and confocal microscopy. Epithelial restitution was analyzed using a scratch wound healing assay. Results E-cadherin-upregulator and ML327 treatment of intestinal epithelial cell monolayers resulted in several barrier-protective effects, including reduced steady-state epithelial permeability, inhibition of cytokine-induced barrier disruption and junction disassembly, and acceleration of epithelial wound healing. Surprisingly, these effects were not due to upregulation of E-cadherin expression but were mediated by multiple mechanisms including inhibition of junction protein endocytosis, attenuation of cytokine-induced apoptosis, and activation of promigratory Src and AKT signaling. Conclusions Our data highlight ECU and ML327 as promising compounds for developing new therapeutic strategies to protect the integrity and accelerate the restitution of the intestinal epithelial barrier in IBD and other inflammatory disorders.

Oxygen free radical injury of IEC-18 small intestinal epithelial cell monolayers

1991 ◽  
Vol 100 (6) ◽  
pp. 1533-1543 ◽  
Author(s):  
Thomas Y. Ma ◽  
Daniel Hollander ◽  
Doug Freeman ◽  
Thang Nguyen ◽  
Pavel Krugliak
Keyword(s):  
Free Radical ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Oxygen Free Radical ◽  
Intestinal Epithelial ◽  
Cell Monolayers ◽  
Small Intestinal Epithelial Cell ◽  
Epithelial Cell Monolayers ◽  
Small Intestinal

scholarly journals Generation of renewable mouse intestinal epithelial cell monolayers and organoids for functional analyses

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Emily C. Moorefield ◽  
R. Eric Blue ◽  
Nancy L. Quinney ◽  
Martina Gentzsch ◽  
Shengli Ding
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers ◽  
Functional Analyses

Polarized transport of docetaxel and vinblastine mediated by P-glycoprotein in human intestinal epithelial cell monolayers

1994 ◽  
Vol 48 (7) ◽  
pp. 1528-1530 ◽  
Author(s):  
Pierre Wils ◽  
Véronique Phung-Ba ◽  
Armelle Warnery ◽  
Delphine Lechardeur ◽  
Shamsi Raeissi ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cell ◽  
Cell Monolayers ◽  
P Glycoprotein ◽  
Epithelial Cell Monolayers ◽  
Polarized Transport

scholarly journals Generation of Human iPSC–Derived Intestinal Epithelial Cell Monolayers by CDX2 Transduction

2019 ◽  
Vol 8 (3) ◽  
pp. 513-526 ◽  
Author(s):  
Kazuo Takayama ◽  
Ryosuke Negoro ◽  
Tomoki Yamashita ◽  
Kanae Kawai ◽  
Moe Ichikawa ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers ◽  
Human Ipsc

Establishment of Intestinal Epithelial Cell Monolayers and Their Use in Calcium Switch Assay for Assessment of Intestinal Tight Junction Assembly

2021 ◽  
Author(s):  
Pawin Pongkorpsakol ◽  
Wilasinee Satianrapapong ◽  
Preedajit Wongkrasant ◽  
Peter R. Steinhagen ◽  
Nuttha Tuangkijkul ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Tight Junction ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers
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