scholarly journals A collagen extraction and deuterium oxide stable isotope tracer method for the quantification of bone collagen synthesis rates in vivo

2021 ◽  
Vol 9 (10) ◽  
Author(s):  
Rita Civil ◽  
Matthew S. Brook ◽  
Kirsty J. Elliott‐Sale ◽  
Lívia Santos ◽  
Ian Varley ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Collagen Synthesis ◽  
Deuterium Oxide ◽  
Bone Collagen ◽  
Tracer Method ◽  
Stable Isotope Tracer ◽  
Isotope Tracer ◽  
Bone Collagen Synthesis ◽  
Isotope Tracer Method

Dynamics of selenite metabolism in young men: studies with the stable isotope tracer method

1984 ◽  
Vol 40 (2) ◽  
pp. 208-218 ◽  
Author(s):  
M Janghorbani ◽  
L J Kasper ◽  
V R Young
Keyword(s):  
Stable Isotope ◽  
Young Men ◽  
Tracer Method ◽  
Stable Isotope Tracer ◽  
Isotope Tracer ◽  
Isotope Tracer Method

scholarly journals Using a dual-stable isotope tracer method to study the uptake, xylem transport and distribution of Fe and its chelating agent from stereoisomers of an Fe(iii)-chelate used as fertilizer in Fe-deficient Strategy I plants

Metallomics ◽  
2010 ◽  
Vol 2 (9) ◽  
pp. 646 ◽  
Author(s):  
Irene Orera ◽  
José A. Rodríguez-Castrillón ◽  
Mariella Moldovan ◽  
José I. García-Alonso ◽  
Anunciación Abadía ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Chelating Agent ◽  
Tracer Method ◽  
Stable Isotope Tracer ◽  
Isotope Tracer ◽  
Xylem Transport ◽  
Strategy I ◽  
Isotope Tracer Method

A stable isotope tracer method to measure silicic acid uptake by marine phytoplankton

1977 ◽  
Vol 78 (1) ◽  
pp. 139-147 ◽  
Author(s):  
David M. Nelson ◽  
John J. Goering
Keyword(s):  
Stable Isotope ◽  
Silicic Acid ◽  
Marine Phytoplankton ◽  
Acid Uptake ◽  
Tracer Method ◽  
Stable Isotope Tracer ◽  
Isotope Tracer ◽  
Isotope Tracer Method

scholarly journals A Dual Stable Isotope Tracer Method for the Measurement of Surfactant Disaturated-Phosphatidylcholine Net Synthesis in Infants with Congenital Diaphragmatic Hernia

2004 ◽  
Vol 56 (2) ◽  
pp. 184-190 ◽  
Author(s):  
Paola E Cogo ◽  
Luc J I Zimmermann ◽  
Giovanna Verlato ◽  
Paola Midrio ◽  
Antonella Gucciardi ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Congenital Diaphragmatic Hernia ◽  
Diaphragmatic Hernia ◽  
Tracer Method ◽  
Stable Isotope Tracer ◽  
Isotope Tracer ◽  
Isotope Tracer Method

scholarly journals Amyloid-β Plaques in Clinical Alzheimer’s Disease Brain Incorporate Stable Isotope Tracer In Vivo and Exhibit Nanoscale Heterogeneity

2018 ◽  
Vol 9 ◽  
Author(s):  
Norelle C. Wildburger ◽  
Frank Gyngard ◽  
Christelle Guillermier ◽  
Bruce W. Patterson ◽  
Donald Elbert ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Stable Isotope ◽  
Amyloid Β ◽  
Stable Isotope Tracer ◽  
Isotope Tracer

scholarly journals Bioequivalence Assessment of High-Capacity Polymeric Micelle Nanoformulation of Paclitaxel and Abraxane® in Rodent and Non-Human Primate Models Using a Stable Isotope Tracer Assay

2021 ◽  
Author(s):  
Duhyeong Hwang ◽  
Natasha Vinod ◽  
Sarah L. Skoczen ◽  
Jacob D. Ramsey ◽  
Kelsie S. Snapp ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Drug Exposure ◽  
Polymeric Micelles ◽  
Clinical Investigation ◽  
Rhesus Macaques ◽  
Physicochemical Characterization ◽  
Polymeric Micelle ◽  
Stable Isotope Tracer ◽  
Isotope Tracer

AbstractThe in vivo fate of nanoformulated drugs is governed by the physicochemical properties of the drug and the functionality of nanocarriers. Nanoformulations such as polymeric micelles, which physically encapsulate poorly soluble drugs, release their payload into the bloodstream during systemic circulation. This results in three distinct fractions of the drug-nanomedicine: encapsulated, protein-bound, and free drug. Having a thorough understanding of the pharmacokinetic (PK) profiles of each fraction is essential to elucidate mechanisms of nanomedicine-driven changes in drug exposure and PK/PD relationships pharmacodynamic activity. Here, we present a comprehensive preclinical assessment of the poly(2-oxazoline)-based polymeric micelle of paclitaxel (PTX) (POXOL hl-PM), including bioequivalence comparison to the clinically approved paclitaxel nanomedicine, Abraxane®. Physicochemical characterization and toxicity analysis of POXOL hl-PM was conducted using standardized protocols by the Nanotechnology Characterization Laboratory (NCL). The bioequivalence of POXOL hl-PM to Abraxane® was evaluated in rats and rhesus macaques using the NCL’s established stable isotope tracer ultrafiltration assay (SITUA) to delineate the plasma PK of each PTX fraction. The SITUA study revealed that POXOL hl-PM and Abraxane® had comparable PK profiles not only for total PTX but also for the distinct drug fractions, suggesting bioequivalence in given animal models. The comprehensive preclinical evaluation of POXOL hl-PM in this study showcases a series of widely-applicable standardized studies by NCL for assessing nanoformulations prior to clinical investigation.GRAPHICAL ABSTRACT

Sequential extracts of human bone show differing collagen synthetic rates

2002 ◽  
Vol 30 (2) ◽  
pp. 61-65 ◽  
Author(s):  
J. Babraj ◽  
D.J. Cuthbertson ◽  
P. Rickhuss ◽  
W. Meier-Augenstein ◽  
K. Smith ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Collagen Synthesis ◽  
Type I Collagen ◽  
Human Bone ◽  
Bone Collagen ◽  
Type I ◽  
Human Beings ◽  
Collagen Turnover ◽  
Bone Collagen Synthesis

Type I collagen is the major bone protein. Little is known quantitatively about human bone collagen synthesis in vivo, despite its importance for the understanding of bone formation and turnover. Our aim was to develop a method that could be used for the physiological and pathophysiological investigation of human bone collagen synthesis. We have carried out preliminary studies in patients undergoing hip replacement and in pigs to validate the use of the flooding dose method using 13C- or 15N-labelled proline and we have now refined our techniques to allow them to be used in a normal clinical or physiological setting. The results show that the application of a flooding dose causes bone free-proline labelling to equilibrate with that of blood in pigs and human beings, so that only 150 mg of bone will provide enough sample to prepare and measure the labelling of three fractions of bone collagen (dissolved in NaCl, acetic acid and pepsin/acetic acid) which have the same relative labelling (1.0:0.43:0.1) as measured by GC-combustion-isotope ratio MS. The rates of incorporation were substantially faster than in skeletal muscle samples taken at the same time. The results suggest that different fractions of human bone collagen turnover at markedly higher rates than had been previously considered. This approach should allow us to discover how growth and development, food, activity and drugs affect bone collagen turnover and to measure the effects on it of ageing and bone disease.

scholarly journals A novel stable isotope tracer method to simultaneously quantify skeletal muscle protein synthesis and breakdown

2020 ◽  
Vol 5 ◽  
pp. 100022 ◽  
Author(s):  
Hannah Crossland ◽  
Kenneth Smith ◽  
Philip J. Atherton ◽  
Daniel J. Wilkinson
Keyword(s):  
Skeletal Muscle ◽  
Protein Synthesis ◽  
Muscle Protein ◽  
Muscle Protein Synthesis ◽  
Skeletal Muscle Protein ◽  
Tracer Method ◽  
Stable Isotope Tracer ◽  
Isotope Tracer ◽  
Skeletal Muscle Protein Synthesis ◽  
Isotope Tracer Method

scholarly journals Quantifications of Lipid Kinetics In Vivo Using Stable Isotope Tracer Methodology

2020 ◽  
Vol 9 (1) ◽  
pp. 110 ◽  
Author(s):  
Il-Young Kim ◽  
Sanghee Park ◽  
Jiwoong Jang ◽  
Robert R. Wolfe
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Tracer ◽  
Isotope Tracer ◽  
Kinetics In Vivo
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