scholarly journals IL‐6 signaling pathway contributes to exercise pressor reflex in rats with femoral artery occlusion in association with Kv4 activity in muscle afferent nerves

2021 ◽  
Vol 9 (13) ◽  
Author(s):  
Qin Li ◽  
Lu Qin ◽  
Jianhua Li
Keyword(s):  
Signaling Pathway ◽  
Femoral Artery ◽  
Artery Occlusion ◽  
Exercise Pressor Reflex ◽  
Afferent Nerves ◽  
Femoral Artery Occlusion ◽  
Muscle Afferent ◽  
Pressor Reflex

scholarly journals Blockade of acid sensing ion channels attenuates the augmented exercise pressor reflex in rats with chronic femoral artery occlusion

2011 ◽  
Vol 589 (24) ◽  
pp. 6173-6189 ◽  
Author(s):  
Hirotsugu Tsuchimochi ◽  
Katsuya Yamauchi ◽  
Jennifer L. McCord ◽  
Marc P. Kaufman
Keyword(s):  
Ion Channels ◽  
Femoral Artery ◽  
Artery Occlusion ◽  
Exercise Pressor Reflex ◽  
Femoral Artery Occlusion ◽  
Acid Sensing Ion Channels ◽  
Pressor Reflex

scholarly journals Tempol attenuates the exercise pressor reflex independently of neutralizing reactive oxygen species in femoral artery ligated rats

2011 ◽  
Vol 111 (4) ◽  
pp. 971-979 ◽  
Author(s):  
Jennifer L. McCord ◽  
Hirotsugu Tsuchimochi ◽  
Katsuya Yamauchi ◽  
Anna Leal ◽  
Marc P. Kaufman
Keyword(s):  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Femoral Artery ◽  
Reactive Oxygen ◽  
Artery Occlusion ◽  
Arterial Supply ◽  
Oxygen Species ◽  
Exercise Pressor Reflex ◽  
Femoral Artery Occlusion ◽  
Pressor Reflex

In decerebrate rats, we reported previously that the exercise pressor reflex arising from a limb whose femoral artery was occluded for 72 h before the experiment was significantly higher than the exercise pressor reflex arising from a contralateral freely perfused limb. These findings prompted us to examine whether reactive oxygen species contributed to the augmented pressor reflex in rats with femoral artery occlusion. We found that the pressor reflex arising from the limb whose femoral artery was occluded for 72 h before the experiment (31 ± 5 mmHg) was attenuated by tempol (10 mg), a superoxide dismutase (SOD) mimetic (18 ± 5 mmHg, n = 9, P < 0.05), that was injected into the arterial supply of the hindlimb. In contrast, the pressor reflex arising from a freely perfused hindlimb (20 ± 3 mmHg) was not attenuated by tempol (17 ± 4 mmHg, n = 10, P = 0.49). Nevertheless, we found no difference in the increase in 8-isoprostaglandin F2α levels, an index of reactive oxygen species, in response to contraction between freely perfused (3.76 ± 0.82 pg/ml, n = 19) and 72-h occluded (3.51 ± 0.92 pg/ml, n = 22, P = 0.90) hindlimbs. Moreover, tempol did not reduce the 8-isoprostaglandin F2α levels during contraction in either group ( P > 0.30). A second SOD mimetic, tiron (200 mg/kg), had no effect on the exercise pressor reflex in either the rats with freely perfused hindlimbs or in those with occluded femoral arteries. These findings suggest that tempol attenuated the exercise pressor reflex in the femoral artery-occluded hindlimb by a mechanism that was independent of its ability to scavenge reactive oxygen species.

scholarly journals Chronic femoral artery occlusion augments exercise pressor reflex in decerebrated rats

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Hirotsugu Tsuchimochi ◽  
Jennifer L McCord ◽  
Shawn G Hayes ◽  
Satoshi Koba ◽  
Marc P Kaufman
Keyword(s):  
Femoral Artery ◽  
Artery Occlusion ◽  
Exercise Pressor Reflex ◽  
Femoral Artery Occlusion ◽  
Pressor Reflex

scholarly journals TRPA1 Function in Skeletal Muscle Sensory Neurons Following Femoral Artery Occlusion

2017 ◽  
Vol 42 (6) ◽  
pp. 2307-2317 ◽  
Author(s):  
Jihong Xing ◽  
Jianhua Li
Keyword(s):  
Femoral Artery ◽  
Transient Receptor Potential ◽  
Artery Occlusion ◽  
Medium Size ◽  
Current Response ◽  
Drg Neurons ◽  
Arterial Blood ◽  
Afferent Nerves ◽  
Femoral Artery Occlusion ◽  
Muscle Afferent

Background/Aims: Transient receptor potential channel A1 (TRPA1) is engaged in amplified autonomic responses evoked by stimulation of muscle afferent nerves in rats with experimental peripheral arterial disease. The purposes of this study were to characterize current responses induced by activation of TRPA1 in dorsal root ganglion (DRG) neurons of control limbs and limbs with femoral artery occlusion. Methods: DRG neurons from rats were labeled by injecting the fluorescence tracer DiI into the hindlimb muscles and whole-cell patch clamp experiments were performed to determine TRPA1 currents. Results: Data show that AITC (a TRPA1 agonist) from the concentrations of 50 µM to 200 µM produces a dose-dependent increase of amplitudes of inward current responses. Notably, the peak current amplitude induced by AITC is significantly larger in DRG neurons of ligated limbs than that in control limbs. AITC-induced current responses are observed in small and medium size DRG neurons, and there is no difference in size distribution of DRG neurons between control limbs and ligated limbs. However, femoral occlusion increases the percentage of the AITC-sensitive DRG neurons as compared to control. AITC-induced currents in DRG neurons are significantly attenuated by exposure to 10 µM of HC-030031, a potent and selective inhibitor of TRPA1, in both control and femoral occlusion groups. In addition, capsaicin (a TRPV1 agonist) evokes a greater increase in the amplitude of AITC-currents in DRG neurons of ligated limbs than that in control limbs. Conclusions: A greater current response with activation of TRPA1 is developed in muscle afferent nerves when hindlimb arterial blood supply is deficient under ischemic conditions; and TRPV1 is partly responsible for augmented TRPA1 responses induced by arterial occlusion.

scholarly journals PO-126 Inhibition of HIF-1α Alleviates Exaggerated Pressor Response Induced by Static Muscle Contraction in Rats with Peripheral Arterial Disease

2018 ◽  
Vol 1 (4) ◽  
Author(s):  
Jianhua Li ◽  
Jihong Xing
Keyword(s):  
Blood Pressure ◽  
Muscle Contraction ◽  
Optical Density ◽  
Femoral Artery ◽  
Pressor Response ◽  
Artery Occlusion ◽  
Reflex Response ◽  
Exercise Pressor Reflex ◽  
Femoral Artery Occlusion ◽  
Pressor Reflex

Objective Hypoxia-inducible factor 1α (HIF-1α) is a transcription factor mediating adaptive responses to hypoxia and ischemia. A prior work showed that HIF-1α is increased in sensory nerves of rats with femoral artery occlusion. The present study was to examine if the reflex response of blood pressure induced by muscle contraction was altered after injection of HIF-1α inhibitor BAY 87-2243 (BAY87) into the arterial blood supply of the ischemic hindlimb muscles.  Methods A rat model of femoral artery ligation was used to study peripheral artery disease. Western blot analysis was employed to examine the protein levels of HIF-1α in the dorsal root ganglion (DRG) tissues. The exercise pressor reflex was evoked by static muscle contraction.  Results HIF-1α was increased in the DRG of occluded limbs (optical density: 0.89±0.13 in control vs. 1.5±0.05 in occlusion; P < 0.05, n=6 in each group). Arterial injection of BAY87 (0.2 mg/kg) inhibited expression of HIF-1α in the DRG of occluded limbs three hours following its injection (optical density: 1.02±0.09 in occluded limbs with BAY87 vs. 1.06±0.1 in control limbs; P > 0.05, n=5 in each group). In addition, muscle contraction evoked a greater increase in blood pressure in occluded rats. BAY87 attenuated the enhanced pressor response in occluded rats to a greater degree than in control rats. Conclusions Inhibition of HIF-1α alleviates exaggeration of the exercise pressor reflex in rats under ischemic circumstances of the hindlimbs induced by femoral artery occlusion.   

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