Interorganisational networks and social innovation: a study in milk production chain

Author(s):  
Marcelo Tsuguio Okano
2017 ◽  
Vol 65 (20) ◽  
pp. 4200-4208 ◽  
Author(s):  
Maurizio Aceto ◽  
Davide Musso ◽  
Elisa Calà ◽  
Fabio Arieri ◽  
Matteo Oddone

2020 ◽  
Vol 48 ◽  
Author(s):  
Érica Chaves Lucio ◽  
Gisele Veneroni Gouveia ◽  
Mateus Matiuzzi Da Costa ◽  
Mário Baltazar De Oliveira ◽  
Rinaldo Aparecido Mota ◽  
...  

Background: Bovine mastitis remains one of the health problems that cause the most damage to milk producers. The negative impact of mastitis is due to reduced milk production, early slaughter of females, reduced commercial value of the affected animals, losses in the genetic potential of the herd, expenses with medicines and veterinary medical assistance. Staphylococcus spp. stands out as the cause of this disease and has been able to remain in the mammary gland, becoming resistant to several antimicrobials. The aims of the present study were to characterize the phenotypes, genotypes and resistance profiles of Staphylococcus spp. isolates from bovine mastitis cases in the state of Pernambuco, Brazil.Materials, Methods & Results: These isolates were classified according to biochemical tests and the presence of the nuc gene.  The polymerase chain reaction (PCR) for amplification of the mecA and blaZ genes was used to analyze the genetic potentials of antimicrobial resistance. Isolates were also phenotypically tested for resistance to nine antimicrobials (ampicillin, doxicillin, erythromycin, gentamicin, rifampicin, cephalothin, amoxicillin, nalidixic acid and oxacillin). The genetic potentials for biofilm production were evaluated by the amplifications of the icaD, icaA and bap genes. The phenotypic test of gentian violet was used for biofilm formation analyzes. Ninety-three (93.0%) of the isolates among the Staphylococcus spp. samples were classified as Staphylococcus aureus. The lowest percentage of sensitivity observed was for amoxicillin (28.0%). All of the isolates were sensitive to erythromycin and gentamicin, and 15 (15%) exhibited sensitivity to all of the drugs tested. All of the isolates were negative for the mecA gene, and 36 (36%) were positive for blaZ. In the adhesion microplate tests, 44 (44%) of the isolates were capable of biofilm formation. Of these, seven (15.9%) were strong formers, whereas 16 (36.3%) and 21 (47.8%) were moderate and weak formers, respectively. The icaD gene was confirmed in 89 (89%) of the isolates. The icaA gene was confirmed in 61 (61%) samples, and the bap gene in 52 (52%) samples. One of the samples did not possess icaA, icaD or bap and exhibited moderate biofilm formation according to the microplate adherence test. Sixteen isolates simultaneously exhibited the three genes tested for biofilm production (icaA, icaD and bap) and were negative according to the microplate adherence test.Discussion:  The indiscriminate use of antibiotics to treat mastitis is a common practice in the study area, which may have contributed to the high proportion of herds (88.23%; 15/17) with multi-resistant isolates, constituting a selection factor for the dissemination of resistant bacteria among herds.  The absence of the mecA gene in the present study may be associated with the development of resistant bacteria through another mechanism, such as the overproduction of beta-lactamases. The results demonstrate that antimicrobial resistance occurs in Staphylococcus spp. that cause bovine mastitis in herds of Pernambuco and that these isolates have the a great capacity for biofilm formation. It is necessary to sensitize the professionals involved in the milk production chain of Brazil regarding the importance of the adequate use of antimicrobials for the treatment and control of mastitis, since studies in the country indicate the dissemination of resistant bacterial strains.


2013 ◽  
pp. 254-267 ◽  
Author(s):  
Renata Giovinazzo Spers ◽  
James Terence Coulter Wright ◽  
André de Azevedo Amedomar

2008 ◽  
Vol 37 (4) ◽  
pp. 773-781 ◽  
Author(s):  
Edizio Santos Junior ◽  
Ricardo Augusto Mendonça Vieira ◽  
Douglas Sampaio Henrique ◽  
Alberto Magno Fernandes

A survey was done based on 19 goat shepherds at counties of Centre-highlands, Northern and North-western regions of the Rio de Janeiro State and at the county of Pedra Dourada, Zona da Mata region, State of Minas Gerais. We aimed to characterise the primary sector of the goat milk production chain settled at those regions. Therefore, questionnaires were applied in order to depict profiles of the shepherds, their families, the role of the wife in the activity, the resources available, dependence on income generated by the activity, and how producers administrate their business. Farms were distributed in five strata according to the following daily milk production averages and standard deviations: 8.8 ± 0.9, 15.7 ± 3.9, 22.6 ± 2.7, 34.4 ± 3.4, and 183.8 ± 54.2 L/d. Approximately 42% of the interviewed producers conducted their activities according to a household production model and the income earned was exclusively from the dairy goat husbandry. Sons and daughters performed an important role in the business (27.80%), but most of them (62.73%) worked out at non farm activities. The percentage of wives that worked directly in the activity (<FONT FACE=Symbol>@</FONT>47%) indicated that it could contribute to gender equity in the rural environment. Most of the production systems (63.16%) presented positive gross margins. We have noticed, however, that shepherds perceived only the business gross margin and that the most accurate registries taken were those related to revenues. In general, producers of the higher strata were favoured by their larger production scale, but asymptotic behaviours for costs and amounts invested in animals, equipments and buildings were observed. These characteristics should be considered when policies related to the dairy goat primary sector have to be planned.


2018 ◽  
Vol 38 (9) ◽  
pp. 1742-1751 ◽  
Author(s):  
Magna C. Lima ◽  
Marina C.C. Souza ◽  
Isis F. Espeschit ◽  
Pedro A.C.C. Maciel ◽  
Jéssica E. Sousa ◽  
...  

ABSTRACT: The Zona da Mata of Minas Gerais has a specialized goat milk production chain. Goat milk is superior in quality compared with milk of other domestic species, and the demand for milk and milk products for the public has increased. Data on dairy goat breeding in Minas Gerais are scarce and relatively old, and this lack of information has limited the implementation of prophylactic measures, especially for mastitis, which represents the biggest sanitary problem for dairy herds. The objective of this work was to characterize mastitis and bacteria associated with it in milking goats in the Zona da Mata of Minas Gerais. It also causes socioeconomic problems and market issues for dairy goat farming. A total of 539 lactating goats were examined and 268 individual samples (one for teat) were collected from animals positive for strip cup test and/or the California Mastitis Test (CMT). Microbiological cultures were carried out on blood agar medium and the bacteria were subjected to phenotypic, genotypic and antimicrobial susceptibility tests. The prevalence of subclinical mastitis was 28.0% and the clinical prevalence was 2.8%. Bacterial multiplication was obtained in 62% of samples. One hundred eighty seven total bacteria were identified. The most common species identified was Staphylococcus aureus (60%), followed Staphylococcus epidermidis (9.1%,), Escherichia coli (6.9%), Staphylococcus saprophyticus (5.9%) e Staphylococcus caprae (4.3%). Bacteria of the genus Staphylococcus presented a profile of resistance to antimicrobials belonging to the beta-lactam class (penicillin, ampicillin and oxacillin) in addition to tetracycline, in contrast to the other antimicrobials tested. Twelve percent of multidrug resistence (MDR) was found in five microregions. Among the bacteria with the highest prevalence of MDR, 38.5% were E. coli and 10.6% were S. aureus. The producers of the Zona da Mata of Minas Gerais are technicians who work with specialized dairy breeds and practise good management. However, some measures related to prophylaxis and control of diseases, such as vaccination, have low adherence or are not performed due to a lack of veterinary assistance. This is the first study focusing on this region, which is highly prominent in goat milk production in Brazil. It provides important information that can help in the implementation of measures for the prophylaxis and control of diseases, and for maintenance of a constant supply of products in sufficient quantities and of a quality suitable for the consumer population.


2010 ◽  
Vol 60 (2) ◽  
pp. 373-376 ◽  
Author(s):  
Marcelo Augusto Ferraz ◽  
Mônica M. O. P. Cerqueira ◽  
Marcelo Resende Souza

Sign in / Sign up

Export Citation Format

Share Document