Determination of the plasma cobalamins by one-dimensional thin-layer chromatography

Vitamin B12 ◽  
1979 ◽  
pp. 665-670 ◽  
Author(s):  
P. Gimsing ◽  
E. Hippe ◽  
Ebba Nexø
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
One Dimensional ◽  
Layer Chromatography

scholarly journals The New TLC Method for Separation and Determination of Multicomponent Mixtures of Plant Extracts

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Elżbieta Matysik ◽  
Anna Woźniak ◽  
Roman Paduch ◽  
Robert Rejdak ◽  
Beata Polak ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Gradient System ◽  
Two Dimensional ◽  
Phase Gradient ◽  
One Dimensional ◽  
Dominant Component ◽  
Phase Condition ◽  
Layer Chromatography

The new mode of two-dimensional gradient thin layer chromatography (MGD-2D TLC) has been presented. Short distance development of sample in the first dimension leads to formation of the preconcentrated narrow zones. They are consecutively separated in the second dimension with the mobile phase gradient in several steps of development until the eluent reaches the further end of the chromatographic plate. The use of the above-mentioned technique allows isolating and then identifying the compounds of various polarity from the multicomponent mixture. The practical application of two-dimensional gradient thin layer chromatography has been performed for isolation of the two plant (JuniperusandThymus) oils components as the examples of test mixtures. The experiments have been carried out with the use of silica gel plates as well as a normal phase condition. The results of solute separation with isocratic one-dimensional thin layer chromatography system have been compared with those of two-dimensional gradient system. It has been observed that application of the latter mode leads to almost triplicated number of zones in comparison with the former one. It is purposeful to apply the proposed mode to control the purity of the dominant component or components of the mixture.

Thin Layer Chromatography and Densitometric Determination of Aflatoxins in Mixed Feeds Containing Citrus Pulp

1986 ◽  
Vol 69 (4) ◽  
pp. 690-696
Author(s):  
Linda J Pennington
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Detection Level ◽  
One Dimensional ◽  
Citrus Pulp ◽  
Relative Standard ◽  
Minimum Detection Level ◽  
Mixed Feeds ◽  
Layer Chromatography

Abstract A method for the accurate one-dimensional thin layer chromatographic (TLC) determination of aflatoxins B1, B2, G1, and G2 in mixed feeds is presented. The aflatoxins are extracted from the sample with chloroform and purified by solvent partitioning. Each aflatoxin is separated from pulp interference by thin layer chromatography on aluminumbacked silica plates. The separated aflatoxins are detected by fluorescence densitometry. Average recoveries for samples spiked from 10 to 100 ppb B1 and G1 and from 3 to 30 ppb B2 and G2 are 82, 84, 95, and 94% for B1, B2, G1, and G2, respectively. The above recovery data, when analyzed for overall method repeatability, produced relative standard deviations of 6.8, 4.3, 6.9, and 7.6% for B1, B2, G1, and G2, respectively. Minimum detection level is less than 1 ppb for each aflatoxin. B1 is confirmed by trifluoroacetic acid derivative formation on a silica TLC plate

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