RAPID CHANGES IN THE AFFINITY BETWEEN CON-A-SEPHAROSE AND LYSOSOMAL ENZYMES OF HUMAN SALIVA DURING THE DAY

The abilities of plant lectins to preferentially agglutinate malignant cells and to bind to specific monosaccharide or oligosaccharide sequences of glycoproteins and glycolipids make them a new and important biochemical probe for investigating alterations in plasma membrane structure which may result from malignant transformation. Electron and light microscopic studies have demonstrated clustered binding sites on surfaces of SV40-infected or tryp- sinized 3T3 cells when labeled with concanavalin A (con A). No clustering of con A binding sites was observed in normal 3T3 cells. It has been proposed that topological rearrangement of lectin binding sites into clusters enables con A to agglutinate SV40-infected or trypsinized 3T3 cells (1). However, observations by other investigators have not been consistent with this proposal (2) perhaps due to differences in reagents used, cell culture conditions, or labeling techniques. The present work was undertaken to study the lectin binding properties of normal and RNA tumor virus-infected cells and their associated viruses using lectins and ferritin-conjugated lectins of five different specificities.

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Molecular analysis during con a-induced liver failure suggests the relevance of jun kinase and IRF-1 for hepatocyte injury

Journal of Hepatology ◽

10.1016/s0168-8278(01)80353-6 ◽

2001 ◽

Vol 34 (0) ◽

pp. 99

Author(s):

K Streetz

Keyword(s):

Liver Failure ◽

Molecular Analysis ◽

Con A ◽

Jun Kinase ◽

Hepatocyte Injury

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Concanavalin A-induced Aggregation of Human Blood Platelets

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647887 ◽

1975 ◽

Vol 33 (02) ◽

pp. 354-360 ◽

Cited By ~ 4

Author(s):

Heinrich Patscheke ◽

Reinhard Brossmer

Keyword(s):

Concanavalin A ◽

Binding Capacity ◽

Specific Binding ◽

Blood Platelets ◽

Residual Effect ◽

Independent Effect ◽

Con A ◽

Experimental Conditions ◽

Main Effect ◽

Induced Aggregation

SummaryConcanavalin A (CON A) causes platelets to aggregate. A Ca++-independent effect of CON A could be separated from a main effect which depends on Ca++. The main effect probably is a consequence of the CON A-induced platelet release reaction and therefore is platelet-specific. The weak residual effect observed in the presence of Na2EDTA may be due to a similar mechanism as has been demonstrated for CON A-induced aggregations of several other normal and malignant transformed animal cells.Na2EDTA did not inhibit the carbohydrate-specific binding capacity of CON A. Therefore, Na2EDTA appears not to demineralize the CON A molecules under these experimental conditions.α-methyl-D-glucoside inhibits the Ca++-independent as well as the Ca++-dependent effect of CON A.Pretreatment by neuraminidase stimulated the platelet aggregation induced by CON A. It is possible that removal of terminal sialic acid residues makes additional receptors accessible for the binding of CON A.

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Economic Way of Thought and Legislationon Nonprofit Organizations

Voprosy Ekonomiki ◽

10.32609/0042-8736-2012-8-48-63 ◽

2012 ◽

pp. 48-63

Author(s):

L. Yakobson

Keyword(s):

Nonprofit Organizations ◽

Empirical Data ◽

Nonprofit Sector ◽

Social Medium ◽

The State ◽

Rapid Changes ◽

Comparative Advantages ◽

Essential Prerequisite

The article considers proper legislation as an essential prerequisite for actualization of NPOs comparative advantages. Restrictions imposed on NPOs are reasonable if they are compensated by benefits from greater trust. The rigidity of constrains and requirements should be optimized while accounting for peculiarities of a social medium, the state of the nonprofit sector, and the governments readiness to encourage the development of the latter. As empirical data suggests, Russian NPOs being on different stages of maturity need separate legal treatment. In the meanwhile, interests that prevail in the NPOs community are not always conducive to rapid changes.

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Section 6.a Introduction

Conference Proceedings of the Academy for Design Innovation Management ◽

10.33114/adim.2017.241 ◽

2019 ◽

Vol 1 (1) ◽

Author(s):

Rebecca PRICE ◽

Christine DE LILLE ◽

Cara WRIGLEY ◽

Kees DORST

Keyword(s):

Case Studies ◽

Organizational Leadership ◽

Research Community ◽

Design Management ◽

Management Research ◽

Research Methodologies ◽

Organizational Research ◽

Research Perspectives ◽

Rapid Changes

There is an increasing need for organizations to adapt to rapid changes in society. This need requires organizations’ and the leader within them, to explore, recognize, build and exploit new capabilities. Researching such capabilities has drawn attention from the design management research community in recent years. Dominantly, research contributions have focused on perspectives of innovation and the strategic application of design with the researcher distanced from context. Descriptive and evaluative case studies of past organizational leadership have been vital, by building momentum for the design movement. However, there is a need now to progress toward prescriptive and explorative research perspectives that embrace context through practice and the simultaneous research of design. Therefore, the aim of this track is to lead and progress discussion on research methodologies that support the research community in developing explorative and prescriptive research methodologies for context-orientated organizational research. This track brings together a group of diverse international researchers and practitioners to fuel discussion on design approaches and subsequent outcomes of prescriptive and explorative research methodologies.

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