Radioimmunoassay for plasma C-type natriuretic peptide determination: a methodological evaluation

Author(s):  
Silvia Del Ry ◽  
Maristella Maltinti ◽  
Michele Emdin ◽  
Claudio Passino ◽  
Giosué Catapano ◽  
...  

AbstractC-Type natriuretic peptide (CNP) is a member of the family of natriuretic peptides with vasodilatory properties, and is produced and secreted by endothelial cells. It seems to play a central role in the paracrine vasomotor control of tone and to be important in several clinical conditions characterized by endothelial dysfunction. We evaluated the analytical performance of a commercially available radioimmunoassay for CNP after a preliminary extraction with Sep-Pak C18. Its analytical reliability was checked by determination of CNP plasma levels in healthy subjects (n=23) and in patients with different diseases, likely characterized by endothelial dysfunction, such as chronic heart failure (n=133) and cirrhosis (n=84). The extraction yield was 78±3%. Accuracy of the radiommunological determination was evaluated by dilution (45–370μL of extracted plasma) and recovery tests (>80%). Between- and within-assay variabilities were ≤10% and analytical sensitivity was 0.41±0.015pg/tube. Plasma CNP in patients with chronic heart failure and with cirrhosis was significantly raised compared to controls (p<0.0001 and p=0.001, respectively). The sensitivity, accuracy and variability levels of the method proposed for CNP assay was suitable for reliable detection of changes in CNP plasma levels in the clinical setting.

2005 ◽  
Vol 7 (7) ◽  
pp. 1145-1148 ◽  
Author(s):  
Silvia Del Ry ◽  
Claudio Passino ◽  
Maristella Maltinti ◽  
Michele Emdin ◽  
Daniela Giannessi

2003 ◽  
Vol 104 (s48) ◽  
pp. 7P-7P
Author(s):  
SG Williams ◽  
DJ Wright ◽  
S Taylor ◽  
ZI Khan ◽  
R O'Brien ◽  
...  

2000 ◽  
Vol 85 (5) ◽  
pp. 1834-1840 ◽  
Author(s):  
Yasushi Moriyama ◽  
Hirofumi Yasue ◽  
Michihiro Yoshimura ◽  
Yuji Mizuno ◽  
Koichi Nishiyama ◽  
...  

Abstract Dehydroepiandrosterone sulfate (DHEAS) is the major secretory steroid of the human adrenal glands. The secretion of DHEAS decreases with aging. The incidence of heart failure also rises in the elderly population. We measured the plasma levels of DHEAS and cortisol in 49 patients with chronic heart failure (CHF) and 32 age-matched controls and assessed its relation to plasma levels of A-type natriuretic peptide and B-type natriuretic peptide, biochemical markers of heart failure. Plasma levels of DHEAS were significantly lower in patients with CHF than in controls, whereas there was no significant difference in plasma levels of cortisol between the two groups. In stepwise regression analysis, the plasma level of DHEAS was significantly and independently correlated with age (β = −0.451; P &lt; 0.0001) and the plasma level of B-type natriuretic peptide (β = −0.338; P &lt; 0.001), and the plasma cortisol/DHEAS ratio was significantly and independently correlated with the plasma levels of A-type natriuretic peptide (β = 0.598; P &lt; 0.0001) and thiobarbituric acid-reactive substances (a marker of oxidative stress;β = 0.252; P &lt; 0. 01) and age (β = 0.171; P &lt; 0.05). These results indicate that the plasma levels of DHEAS are decreased in patients with CHF in proportion to its severity and that oxidative stress is associated with decreased levels of DHEAS in patients with CHF.


Critical Care ◽  
2007 ◽  
Vol 11 (Suppl 3) ◽  
pp. P48
Author(s):  
EJ de Almeida Figueiredo ◽  
A Perez ◽  
E Knobel ◽  
JMA Souza ◽  
MB Ferri ◽  
...  

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