Chiral separation of α-cyclohexylmandelic acid enantiomers using ionic liquid/salt aqueous two-phase system

2015 ◽  
Vol 69 (11) ◽  
Author(s):  
Lei-Lei Chen ◽  
Fen-Fang Li ◽  
Zhi-Jian Tan
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Chiral Separation ◽  
Mixing Time ◽  
Chiral Selector ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Liquid Salt ◽  
Influencing Parameters

AbstractIn this paper, an ionic liquid-based aqueous two-phase system (ILATPS) was applied to the chiral separation of α-cyclohexylmandelic acid (α-CHMA) enantiomers with hydroxypropyl-β- cyclodextrin (HP-β-CD) as the chiral selector. Several influencing parameters were investigated including the types and concentration of ionic liquids, the amount of phase-forming salt, temperature, mixing time, pH, and the content of HP-β-CD. The results showed that not all ILATPS had the ability to chirally recognise the selected enantiomers and that [C

Extraction and mechanism investigation of trace roxithromycin in real water samples by use of ionic liquid–salt aqueous two-phase system

2009 ◽  
Vol 653 (2) ◽  
pp. 178-183 ◽  
Author(s):  
Chun-Xiang Li ◽  
Juan Han ◽  
Yun Wang ◽  
Yong-Sheng Yan ◽  
Xiao-Hui Xu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Water Samples ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Real Water Samples ◽  
Liquid Salt

Partitioning Behavior of Penicillin G in Aqueous Two Phase System Based on Ionic Liquids

2013 ◽  
Vol 864-867 ◽  
pp. 324-327
Author(s):  
Bin Jiang ◽  
Zhi Biao Feng ◽  
Chun Hong Liu ◽  
Ying Cao Xu ◽  
Dong Mei Li ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Extraction Yield ◽  
Penicillin G ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Partitioning Behavior

An aqueous two-phase system (ATPS) was presented with hydrophilic ionic liquid 1-Butyl-3-methylimid-azolium tetrafluoroborate ([BmiBF4) and salts for the extraction of penicillin G in this paper. The phase forming abilities of different salts were investigated to choose a suitable salt.The partitioning behavior of penicillin G in the ATPS was investigated.Concentrations of (NH4)2SO4, penicillin G, and [BmiBF4 were evaluated to determine their effects on the extraction yield of penicillin G. It was found that extraction yield strongly depended on the concentration of [BmiBF4, penicillin and (NH4)2SO4. A high extraction yield around 93% was achieved with the following parameters: (NH4)2SO4 20% (wt%), penicillin 40000 ~ 45000 U/mL, [BmiBF4 20% (wt%).

Partition of proteins with extraction in aqueous two-phase system by hydroxyl ammonium-based ionic liquid

2014 ◽  
Vol 6 (12) ◽  
pp. 4067-4076 ◽  
Author(s):  
Jing Chen ◽  
Yuzhi Wang ◽  
Qun Zeng ◽  
Xueqin Ding ◽  
Yanhua Huang
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Ammonium Ionic Liquids

A series of hydroxyl ammonium ionic liquids have been designed and synthesized and used for the extraction of proteins.

The Research for the Extraction of Yeast’S Nucleic Acid with [BMIM] BF4-H2O-KH2PO4 Ionic Liquid Aqueous Two-Phase System

2012 ◽  
Vol 455-456 ◽  
pp. 477-482 ◽  
Author(s):  
De Juan Huang ◽  
De Chao Huang
Keyword(s):  
Ionic Liquid ◽  
Nucleic Acid ◽  
Good Effect ◽  
Control Group ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Liquid Salt ◽  
Naoh Solution ◽  
Phase Process

Construct ionic liquid - salt aqueous two-phase system for extracting yeast DNA. By using NaOH solution to process yeast, leaching the nucleic acid by ultrasonic assisting, variety of salts’ aqueous two-phase influence on the ionic liquid 1-butyl-3-methylimidazolium fluoroborate are discussed, and the influence of additive quantity of KH2PO4 and PH on the ionic liquid aqueous two-phase system for extraction of nucleic acid are studied. The result shows that process the yeast with 0.3% NaOH solution, assisted by ultrasound with power of 80W, temperature 60 °C, 3 hours’ extraction. KH2PO4take obvious advantage in the formation of ionic liquid aqueous two-phase system. In the process of extraction nucleic acid from [BMIM] BF4-H2O-KH2PO4aqueous two-phase process of nucleic acid, the best B-R buffer PH is 8.95, when the additive dosage of KH2PO4is 2.000g, A260/A280 in the supernatant is smaller than control group, the substratum which rich in ionic liquid contains more nucleic acid than the supernatant, and A260 in the supernatant is more than that in the control group. Therefore, we conclude that the ionic liquid aqueous two-phase has a relatively good effect in extraction of nucleic.

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