Optimization of Aqueous Two Phase Extraction of Proteins from Litopenaeus Vannamei Waste by Response Surface Methodology Coupled Multi-Objective Genetic Algorithm

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
P. Saravana Pandian ◽  
S. Sindhanai Selvan ◽  
A. Subathira ◽  
S. Saravanan
Keyword(s):  
Genetic Algorithm ◽  
Molecular Weight ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Litopenaeus Vannamei ◽  
Value Added ◽  
Phase System ◽  
Two Phase ◽  
Multi Objective ◽  
Multi Objective Genetic Algorithm

Abstract Waste generated from industrial processing of seafood is an enormous source of commercially valuable proteins. One among the underutilized seafood waste is shrimp waste, which primarily consists of head and carapace. Litopenaeus vannamei (L. vannamei) is the widely cultivated shrimp in Asia and contributes to 90 % of aggregate shrimp production in the world. This work was focused on extraction as well as purification of value-added proteins from L. vannamei waste in a single step aqueous two phase system (ATPS). Polyethylene glycol (PEG) and trisodium citrate system were chosen for the ATPS owing to their adequate partitioning and less toxic nature. Response surface methodology (RSM) was implemented for the optimization of independent process variables such as PEG molecular weight (2000 to 6000), pH (6 to 8) and temperature (25 to 45 °C). The results obtained from RSM were further validated using a Multi-objective genetic algorithm (MGA). At the optimized condition of PEG molecular weight 2000, pH 8 and temperature 35 °C, maximum partition coefficient and protein yield were found to be 2.79 and 92.37 %, respectively. Thus, L. vannamei waste was proved to be rich in proteins, which could be processed industrially through cost-effective non-polluting ATPS extraction, and RSM coupled MGA could be a potential tool for such process optimization.

scholarly journals Response Surface Methodology for the Evaluation of Magnetite Partitioning Behavior in Polyethylene Glycol-Based Aqueous Biphasic System

2019 ◽  
Vol 43 (6) ◽  
pp. 2807-2813 ◽  
Author(s):  
Behnia Shahrokhi ◽  
Mohsen Pirdashti ◽  
Mozhgan Managhebi
Keyword(s):  
Molecular Weight ◽  
Response Surface Methodology ◽  
Polyethylene Glycol ◽  
Response Surface ◽  
Polynomial Equation ◽  
Weight Percent ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Biphasic ◽  
Partitioning Behavior

Abstract Response surface methodology in the framework of central composite design has successfully applied to polyethylene glycol (PEG)-based aqueous two-phase system in order to evaluate its efficiency in extracting Fe from iron ores with respect to PEG molecular weight, PEG concentration and salt concentration factors. Furthermore, ANOVA and multiple regression analysis have provided a second-order polynomial equation that is highly capable of predicting the experimental results. The PEG molecular weight and weight percent of the salt in the feed had a major and minor effects on the iron ion partition coefficient, respectively.

Effects of Euler Angles of Vertical Cambered Otter Board on Hydrodynamics Based on Response Surface Methodology and Multi-Objective Genetic Algorithm

2020 ◽  
Vol 143 (2) ◽  
Author(s):  
Gang Wang ◽  
Liuyi Huang ◽  
Lei Wang ◽  
Fenfang Zhao ◽  
Xinxin Wang ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Genetic Algorithm ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Center Of Pressure ◽  
Lift Coefficient ◽  
Hydrodynamic Characteristics ◽  
Euler Angles ◽  
Multi Objective ◽  
Multi Objective Genetic Algorithm

Abstract In this present work, effects of three Euler angles (angle of attack (AOA), angle of trim (AOT), and angle of pitch (AOP)) of vertical cambered otter board on hydrodynamic characteristics (drag coefficient (Cd), lift coefficient (Cl), center-of-pressure coefficients (Cp)) were studied based on numerical simulation combined with Kriging response surface methodology (KRSM) and multi-objective genetic algorithm (MOGA). Wind tunnel experiments were carried out to validate the accuracy of the response surface based on numerical simulation. It was demonstrated that AOA had noticeable effects on Cd and Cl, while AOT and AOP had fewer effects. The working posture of the otter board was recommended to lean inward (0 deg–6 deg) and forward (−10 deg–0 deg) to improve the lift-drag ratio without sacrificing Cl. The influences of AOT and AOP on positions of center-of-pressure points were less significant than that of AOA and decreasing with the increase of AOA. Besides, the response surface of hydrodynamic coefficients around the critical AOA was a decent indicator of the occurrence of stall. Finally, three candidate cases were selected to satisfy the high working efficiency by MOGA, which was consistent with the above recommendations. This study provided a scientific reference of response surface experimental investigations methodology in the fishery engineering and the configuration of Euler angles of otter board.

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