MEIOTIC STUDIES IN THE M2 GENERATION OF Helianthus annuus L. VARIETY EKIZ 1 AFTER GAMMA IRRADIATION / LOS ESTUDIOS EN LA GENERACION DE Helianthus annuus L. LA VARIEDAD EKIZ 1 DESPUES DE LA IRRADIACION DE LA GAMMA / APRÉS LE RAYONNEMENT DES GAMMA DANS LA GÉNÉRATION M2 DE LA VARIÉTÉ EKIZ 1 (Helianthus annuus L.)

SUMMARYThe effect of gamma rays on meiosis in the M2 generation has been studied in sunflower (Helianthus annuus L.) variety Ekiz 1. Seeds were irradiated with gamma rays at 10, 20, 30, 40 and 50 kR doses. Radiation induced meotic abnormalities. The chromosomal aberrations included univalents, multivalents and stickiness at diakinesis, laggards and stickiness at metaphase I, bridges and laggards at anaphase and telophase I-II, micronuclei at telophase II. The percentage frequencies of anomalies increased generally with the increasing dose of radiation.

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GAMMA RAY - INDUCED MITOTIC ABNORMALITIES IN Helianthus annuus L. VARIETY EKIZ 1 / LOS RAYOS DE GAMMA CAUSA ANORMALIDADES MITOTICAS EN Helianthus annuus L. VARIEDAD DE EKIZ 1 / LES ANORMALITÉS MITOTIQUES CHARGÉÉS DES RAYONS GAMMA

Helia ◽

10.1515/helia.2001.24.35.39 ◽

2001 ◽

Vol 24 (35) ◽

pp. 39-46

Author(s):

Orhan Arslan ◽

Şenol Bal ◽

Nilgün Venice ◽

Semra Mirici

Keyword(s):

Helianthus Annuus ◽

Mitotic Index ◽

Gamma Rays ◽

Metaphase Chromosome ◽

Gamma Ray ◽

Dose Increase ◽

Helianthus Annuus L ◽

Mitotic Abnormalities ◽

Chromosome Stickiness

SUMMARYIn this study, mitotic effects of gamma rays on Ekiz 1 variety belonging to Helianthus annuus L. (2n= 34) in the M0 (first irradiated seeds), M1 and M2 generations have been investigated. Seeds (M0) were irradiated with gamma rays at 10, 20, 30, 40 and 50 kR doses. Percentage of total abnormalities in the M0, M1 and M2 generations increased parallel to the increasing dose of radiation. These abnormalites have been observed as C-metaphase, chromosome stickiness, laggards and bridges with or without fragment. Mitotic index (M.I.) in the M0, M1 and M2 generations has decreased parallel to the dose increase. When the generations are compared, both the amounts of decrease in mitotic index and in the percentage of mitotic abnormalities were mostly observed in M0.

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Chromosomal Induced Aberrations in Sunflower (Helianthus annuus. L) with Gamma-irradiation, Sodium Azide and Combined Treatments

CYTOLOGIA ◽

10.1508/cytologia.74.253 ◽

2009 ◽

Vol 74 (3) ◽

pp. 253-258

Author(s):

P. Raja Ramesh Kumar ◽

Venkat Ratnam S.

Keyword(s):

Gamma Irradiation ◽

Helianthus Annuus ◽

Sodium Azide ◽

Combined Treatments ◽

Helianthus Annuus L

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Radiation-induced chromosomal aberrations in human lymphocytes after partial-body exposure to60Co gamma-irradiation andin vitroexposure to 230 kV X-irradiation

British Journal of Radiology ◽

10.1259/0007-1285-48-570-487 ◽

1975 ◽

Vol 48 (570) ◽

pp. 487-493 ◽

Cited By ~ 2

Author(s):

Gwyneth E. Watson ◽

N. E. Gillies

Keyword(s):

Gamma Irradiation ◽

Chromosomal Aberrations ◽

Human Lymphocytes ◽

X Irradiation ◽

Radiation Induced ◽

Partial Body

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Radiation-induced chromosomal aberrations in human lymphocytes. I. Dependence on the dose of gamma-rays and an anomaly at low doses

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/0027-5107(76)90246-3 ◽

1976 ◽

Vol 36 (3) ◽

pp. 363-377 ◽

Cited By ~ 35

Author(s):

N.V. Luchnik ◽

A.V. Sev Ankaev

Keyword(s):

Gamma Rays ◽

Chromosomal Aberrations ◽

Human Lymphocytes ◽

Low Doses ◽

Radiation Induced

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SERAPAN OPTIK POLIMER FILM PVA-TCA-MB YANG DIRADIASI SINAR GAMMA

JURNAL PIJAR MIPA ◽

10.29303/jpm.v7i2.96 ◽

2012 ◽

Vol 7 (2) ◽

Author(s):

Ni Nyoman Sri Putu Verawati

Keyword(s):

Optical Properties ◽

Radiation Dose ◽

Gamma Irradiation ◽

Absorption Spectra ◽

Gamma Rays ◽

Colour Change ◽

Strong Acid ◽

Poly Vinyl Alcohol ◽

Radiation Induced ◽

Uv Visible

Abstrak: Pengaruh radiasi sinar gamma terhadap sifat optik dari poli vinil alkohol (PVA) yang dicampur dengan trichloro acetid acid (TCA) dan pewarna methilen blue (MB) dapat digunakan pada dosimetri dan pengukuran dosis radiasi sinar gamma telah dipelajari dengan menggunakan metode spektrofotometer. dosimeter telah diradiasi sampai dosis 14 kGy dengan menggunakan sinar gamma 60Co. Polimer film mengalami perubahan warna dari biru keunguan ke kuning akibat radiasi sinar gamma. Perubahan warna ini mengindikasikan terbentuknya asam kuat, dengan kenaikan dosis radiasi banyak ion klorin putus dari rantai karbon TCA dan akibat radiasi menyebabkan ion H+ dan OH- menjadi radikal bebas yang dihasilkan dari proses hidrolisis air dari molekul sampel, kemudian berkombinasi dalam bentuk asam. spektrum serapan telah diukur menggunakan spektrofotometer Uv-Vis pada rentang panjang gelombang 200-700 nm, menghasilkan penurunan serapan pada puncak 360 dan 440 nm dengan peningkatan dosis dan kenaikan serapan optik pada puncak 560 nm dengan kenaikan dosis radiasi. Kata kunci : Sifat optik, radiasi sinar gamma, polimer film PVA-TCA-MB, spektrum serapan optik. Abstract: The effect of gamma irradiation on optical properties of methylene-blue dyed poly vinyl alcohol (PVA) blended with trichloroacetid acid (TCA) for use in dosimetry and measurement of radiation dose in gamma rays have been studied using Uv-Visible spectrophotometer method. The dosimeters were irradiated for doses up 14 kGy using 60Co gamma rays source at a constant dose rate. The polymeric films undergo colour change from blue purplish to yellow due to radiation-induced. The changes of this colour indicated strong acid, by the increasing of radiation dose caused more chlorine ions break from carbon of TCA and radiation-induced H+ and OH- free radicals are generated from the hydrolysis of water molecules, they then combined to form acid. The absorption spectra were measured using Uv-Visible spectrophotometer in the wavelength range 200-700 nm, resulting in a decrease of the absorbance at 360 and 440 nm band peak with increasing dose and an increases of the absorbance at 560 nm band peak with increasing dose. Keywords: Optical properties, gamma irradiation, polymer film PVA-TCA-MB, optical absorption spectra.

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