Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA – A practical approach

Holzforschung ◽  
2004 ◽  
Vol 58 (2) ◽  
pp. 199-204 ◽  
Author(s):  
N. Högberg ◽  
C. J. Land
Keyword(s):  
Species Identification ◽  
Ribosomal Dna ◽  
Wood Decay ◽  
Close Relative ◽  
Fomitopsis Pinicola ◽  
Decay Fungi ◽  
5.8S Rdna ◽  
Resinicium Bicolor ◽  
Wood Decay Fungi ◽  
Serpula Lacrymans

AbstractWe have approached species identification of wood decay fungi in construction wood by means of sequencing ribosomal DNA. Sequencing of the Internal Transcribed Spacer (ITS), which includes the 5.8S rDNA, is straightforward and provides a basis for species identification. Identification was either by BLAST search on sequences in GenBank or phylogenetic analysis. A number of important wood decay fungi such asSerpula lacrymans, S. himantioides, Antrodia serialis, A. sinuosa, Gloeophyllum sepiarium, Fomitopsis pinicola, Resinicium bicolorandJunghuhnia collabenshave been successfully identified from fruitbodies and directly from wood samples. Sequence variants were found within the species sampled includingS. himantioides, the close relative ofS. lacrymans.But, among 27 samples from fruitbodies and mycelium ofS. lacrymans, unexpectedly, no sequence variation was detected.

Differentiation of indoor wood decay fungi with MALDI-TOF mass spectrometry

Holzforschung ◽  
2005 ◽  
Vol 59 (3) ◽  
pp. 374-377 ◽  
Author(s):  
Olaf Schmidt ◽  
Wibke Kallow
Keyword(s):  
Mass Spectrometry ◽  
Wood Decay ◽  
Decay Fungi ◽  
Maldi Tof Ms ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Wood Decay Fungi ◽  
Serpula Lacrymans ◽  
Tof Ms

Abstract MALDI-TOF MS differentiated mycelia within pairs each of the closely related indoor wood decay fungi Serpula lacrymans, S. himantioides, Coniophoraputena, C. marmorata, and Antrodia vaillantii, A. sinuosa. The method is thus suitable to identify unknown samples by spectrum comparison.

High-performance liquid chromatographic analysis of soluble and total oxalate in Ca- and Mg-amended liquid cultures of three wood decay fungi

Holzforschung ◽  
2004 ◽  
Vol 58 (6) ◽  
pp. 682-687 ◽  
Author(s):  
Jonathan S. Schilling ◽  
Jody Jellison
Keyword(s):  
High Performance ◽  
Wood Decay ◽  
Brown Rot ◽  
High Performance Liquid Chromatographic ◽  
White Rot ◽  
Fomitopsis Pinicola ◽  
Decay Fungi ◽  
Postia Placenta ◽  
Medium Components ◽  
Wood Decay Fungi

AbstractTwo brown-rot wood decay fungi,Fomitopsis pinicolaandMeruliporia incrassata, and the white-rot speciesPhanerochaete chrysosporiumwere grown for 4 weeks in liquid culture at 0.35, 0.70, 1.05, and 5.00 mM calcium (Ca) and 1.35 and 2.70 mM magnesium (Mg) concentrations. Soluble and total oxalate levels were quantified using a revised ion-exchange HPLC protocol developed specifically for resolving oxalate and other organic acid anions from medium components. Total oxalate concentrations in brown-rot filtrate were not significantly different among treatments; however, soluble oxalate decreased significantly with increasing Ca concentration. Higher Mg concentrations increased soluble oxalate levels only slightly. There was a significant decrease in medium pH at 5.00 mM Ca for all species, as well as an apparent increase in decarboxylation activity in brown-rot fungi. Total and soluble oxalate levels in the white-rot cultures were generally below detection for all treatments. The results show a significant influence of Ca on soluble oxalate concentrations not seen previously in the brown-rot speciesPostia placenta.

scholarly journals The comparison of properties of European beech Fagus sylvatica (L.) in different stage of degradation caused by wood-decay fungi

2009 ◽  
Vol 57 (5) ◽  
pp. 119-130
Author(s):  
Jiří Holan ◽  
Blanka Stávková
Keyword(s):  
Fagus Sylvatica ◽  
Compression Strength ◽  
Trametes Versicolor ◽  
Wood Decay ◽  
Brown Rot ◽  
European Beech ◽  
Wood Fibers ◽  
Decay Fungi ◽  
Wood Decay Fungi ◽  
Serpula Lacrymans

This work focus on comparison of biological degradation of wood caused by wood-decay fungi (white and brown rot). Test samples were made of European Beech Fagus sylvatica (L.). As wood-decay fungi were used Trametes versicolor (L.) Lloyd (white rot) and Serpula lacrymans (Wulf. Ex Fr.) Schroet (brown rot). Aim of this work was comparison of rate of propagation of wood-decay fungus and degradation of wood in time. After termination of the test was made comparison of intensity of degradation between both fungi species. Weights of test samples were diminishing for both groups of wood-decay fungi during three months. Moisture content increased in direct proportion with time. Compression strength in direction of wood fibers of tested samples was diminishing. Samples tested by Serpula lacrymans had the fastest decrease of compression strength after first and second week of degradation. Samples tested by Trametes versicolor had different course. Compression strength significantly decreased after first month and third month of degradation. On the other hand module of elasticity of both tested groups was diminishing already during first and second week of degradation. Generally, it is possible to say that Trametes versicolor has more significant impact on changes of mechanical characteristic of wood, because it causes degradation of all chemical constituents of wood.

ANTIFUNGAL ACTIVITY OF CRUDE EXTRACTS OF Euphorbia tirucalli; ISOLATION AND CHARACTERIZATION OF ONE OF ITS BIOACTIVE PRINCIPLES TIRUCALLOL

2021 ◽  
Vol 4 (4) ◽  
pp. 213-222
Author(s):  
David Oriabure Ekhuemelo ◽  
J. V. Anyam ◽  
C. Ekhuemelo
Keyword(s):  
Ethyl Acetate ◽  
Wood Decay ◽  
Dilution Method ◽  
Zone Of Inhibition ◽  
Fomitopsis Pinicola ◽  
Fresh Sample ◽  
Decay Fungi ◽  
Euphorbia Tirucalli ◽  
Isolation And Characterization ◽  
Wood Decay Fungi

Antifungal properties of extracts from Euphorbia tirucalli against some wood decay fungi were studied. E. tirucalli fresh sample was harvested, cleansed and chipped into chips of 3 x 2 cm length and breadth. Chipped sample (846.3 g) was macerated in 1000 mL of ethyl acetate, n hexane and methanol solvents, respectively. Column chromatography experiment on E. tirucalli was carried out and antifungal screening was done according to standard method for 7 days to observe zones of inhibition of fungi growth. Broth dilution method was adopted to determine the Minimum Inhibition Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of E. tirucalli extracts and fraction. Results show that Fibroporia vaillantii, Fomitopsis pinicola, Gloeophyllum sepiarium, Rhizopus sp. and Serpula lacrymans were sensitive to ethyl acetate, n’ hexane and methanol E. tirucalli extracts at concentration of 200 mg/mL with zone of inhibition of  18-26 mm and  methanol extract had the highest value of 26 mm. The fraction had zone of inhibition of between 18 and 20 mm which are not significantly different (p<0.05) from 29-35 mm obtained from antibiotics. The MIC of 10 mg/mL and MFC of 20 mg/mL were recorded for extracts against the test fungi while MIC of 50 µg/mL and MFC 200 µg/mL were noted for fraction (Et15), respectively. Fraction (Et15) obtained from E. tirucalli was characterised as tirucallol compound. In conclusion, E. tirucalli extracts and fraction have proved to be effective in the control of wood decay fungi and may serve as control of diseases caused by the test fungi

Ribosomal DNA intergenic spacer of indoor wood-decay fungi

Holzforschung ◽  
2008 ◽  
Vol 62 (6) ◽  
Author(s):  
Olaf Schmidt ◽  
Ute Moreth
Keyword(s):  
Ribosomal Dna ◽  
Repeat Unit ◽  
Intergenic Spacer ◽  
Wood Decay ◽  
5S Rdna ◽  
Reverse Direction ◽  
Dry Rot ◽  
Decay Fungi ◽  
Wood Decay Fungi ◽  
Rdna Repeat Unit

AbstractIndoor wood-decay fungi are economically very important. Approximately half of the total damage caused by indoor fungi in Germany is caused by species of the Coniophoraceae. The sequence of the intergenic spacer (IGS) region of the ribosomal DNA was elucidated with the following fungi of this family:Serpula lacrymans(true dry rot fungus),S. himantioides(wild merulius),Meruliporia incrassata(North American dry rot fungus),Leuco-gyrophana pinastri(mine dry rot fungus),Coniophora puteana(brown cellar fungus), andC. marmorata(marmoreus cellar fungus). The IGS length ranges between 2584 and 3785 bp and consists of the short IGS 1 (253–440 bp), the 5S rDNA (118 bp) and the long IGS 2 (2193–3310 bp). IGS 1 is phylogenetically less informative for the investigated Coniophoraceae species. 5S rDNA is transcribed in the reverse direction. IGS 2 contains extended repeat blocks of copies of different length. Intraspecific length polymorphism as a result of different copy number occurs inM. incrassataandL. pinastri. In combination with previous results, the full-length sequence of the rDNA repeat unit is available for important indoor wood-decay fungi. The various rDNA regions can now be used for future identification of unknown sequences by BLAST and also for phylogenetic studies.

Growth inhibition of wood-decay fungi by lignin-related aromatic compounds

2021 ◽  
Author(s):  
Cédric Cabral Almada ◽  
Mathilde Montibus ◽  
Frédérique Ham-Pichavant ◽  
Sandra Tapin-Lingua ◽  
Gilles Labat ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Aromatic Compounds ◽  
Wood Decay ◽  
Decay Fungi ◽  
Wood Decay Fungi

Aliphatic acids as spore germination inhibitors of wood-decay fungi in vitro

1985 ◽  
Vol 63 (2) ◽  
pp. 337-339 ◽  
Author(s):  
Elmer L. Schmidt
Keyword(s):  
Spore Germination ◽  
Germ Tube ◽  
White Rot Fungi ◽  
Wood Decay ◽  
White Rot ◽  
Malt Extract ◽  
Decay Fungi ◽  
Aliphatic Acids ◽  
Wood Decay Fungi

Influences of eight saturated aliphatic acids (C5–C10, C12, and C16) on basidiospores of four isolates of wood-decay fungi (Poria tenuis and Trametes hispida, white rot fungi, and two isolates of the brown rot fungus Gloeophyllum trabeum) were observed in vitro. Spore responses after 24 h on malt extract agar containing 10, 102 or 103 ppm of each acid included normal germination, delay of germ tube emergence, vacuolation and degeneration of spore cytoplasm, and prevention of germ tube development without spore destruction. Acids of chain length C5–C10 prevented spore germination and killed spores of all fungi at concentrations of 20–50 ppm in media, whereas other acids tested were less active. Spore germination assay of decay fungi may prove useful as a screening tool to compare potency of wood preservatives.

The role of wood decay fungi in the carbon and nitrogen dynamics of the forest floor

2009 ◽  
pp. 151-181 ◽  
Author(s):  
Sarah Watkinson ◽  
Dan Bebber ◽  
Peter Darrah ◽  
Mark Fricker ◽  
Monika Tlalka ◽  
...  
Keyword(s):  
Forest Floor ◽  
Wood Decay ◽  
Nitrogen Dynamics ◽  
Decay Fungi ◽  
Carbon And Nitrogen ◽  
Wood Decay Fungi ◽  
Carbon And Nitrogen Dynamics

Bioconversion of Heptachlor Epoxide by Wood-Decay Fungi and Detection of Metabolites

2012 ◽  
Vol 518-523 ◽  
pp. 29-33 ◽  
Author(s):  
Peng Fei Xiao ◽  
Toshio Mori ◽  
Ryuichiro Kondo
Keyword(s):  
Wide Spectrum ◽  
Wood Decay ◽  
Heptachlor Epoxide ◽  
Hydroxyl Group ◽  
Epoxide Ring ◽  
Limited Information ◽  
Decay Fungi ◽  
Wood Decay Fungi ◽  
Metabolic Products ◽  
Phlebia Brevispora

Although heptachlor epoxide is one of the most persistent organic pollutants (POPs) that cause serious environmental problems, there is very limited information of the biodegradation of heptachlor epoxide by microorganisms, and no systematic study on the metabolic products and pathway of endrin by microorganisms has been conducted. Wood-decay fungi can degrade a wide spectrum of recalcitrant organopollutants, including polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated biphenyls (PCBs). In this study, 18 wood-decay fungi strains of genus Phlebia were investigated for their ability to degrade heptachlor epoxide, and Phlebia acanthocystis, Phlebia brevispora, Phlebia lindtneri and Phlebia aurea removed about 16, 16, 22 and 25% of heptachlor epoxide, respectively, after 14 days of incubation. Heptachlor diol and 1-hydroxy-2,3-epoxychlordene were detected in these fungal cultures as metabolites by gas chromatography and mass spectrometry (GC/MS), suggesting that the hydrolysis reaction in the epoxide ring and substitution of chlorine atom with hydroxyl group in C1 position occur in bioconversion of heptachlor epoxide by selected wood-decay fungi, respectively. This is the first report describing the metabolites of heptachlor epoxide by microorganisms.

Sign in / Sign up

Export Citation Format

Share Document