Serological survey of the influenza A virus in Polish farrow-to-finish pig herds in 2011–2015

AbstractIntroduction: The aim of this study was to assess the seroprevalence of swine influenza A virus (SIV) in Polish farrow-to-finish pig herds.Material and Methods: Serum samples collected from 5,952 pigs, from 145 farrow-to-finish herds were tested for the presence of antibodies against H1N1, H1N1pdm09, H1N2, and H3N2 SIV subtypes using haemagglutination inhibition (HI) test. Samples with HI titres equal or higher than 20 were considered positive.Results: HI antibodies to at least one of the analysed SIV subtypes were detected in 129 (89%) herds and in 2,263 (38%) serum samples. Antibodies to multiple SIV subtypes were detected in 104 (71.7%) herds and in 996 (16.7%) serum samples. Concerning the seroprevalence rate, according to age category, the highest prevalence of the antibodies was detected in weaners, with regard to the H1N1, H1N2, and H3N2, and in sows, with regard to the H1N1pdm09. The lowest seroprevalence for all evaluated SIV subtypes was detected in finishers.Conclusion: The study indicates that antibodies against single and multiple SIV subtypes are circulating in Polish farrow-to-finish herds and highlights the importance of conducting a molecular surveillance programme in future studies.

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Detection of H1 Swine Influenza A Virus Antibodies in Human Serum Samples by Age Group1

Emerging Infectious Diseases ◽

10.3201/eid2609.191796 ◽

2020 ◽

Vol 26 (9) ◽

pp. 2118-2128 ◽

Cited By ~ 1

Author(s):

Elien Vandoorn ◽

Isabel Leroux-Roels ◽

Geert Leroux-Roels ◽

Anna Parys ◽

Amy Vincent ◽

...

Keyword(s):

Human Serum ◽

Influenza A Virus ◽

Influenza A ◽

Swine Influenza ◽

Serum Samples ◽

Human Serum Samples ◽

Swine Influenza A Virus

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Serological evidence of continuing infection of swine in Great Britain with an influenza A virus (H3N2)

Journal of Hygiene ◽

10.1017/s0022172400024876 ◽

1978 ◽

Vol 80 (3) ◽

pp. 415-422 ◽

Cited By ~ 11

Author(s):

M. S. Chapman ◽

P. H. Lamont ◽

J. W. Harkness

Keyword(s):

Great Britain ◽

Influenza A Virus ◽

Small Proportion ◽

Human Population ◽

Influenza A ◽

Haemagglutination Inhibition ◽

Swine Influenza ◽

Seasonal Fluctuations ◽

Serological Evidence ◽

Serum Samples

SummarySerum samples collected from swine and cattle in Great Britain at various times between July 1971 and July 1977 were examined by haemagglutination-inhibition or single radial haemolysis methods for evidence of infection with influenza A (H3N2) viruses. A small proportion of swine sera collected in each year reacted in the tests but there was no evidence of infection in cattle. The significance of the findings is discussed, with particular reference to the seasonal fluctuations in the prevalence of antibody in swine observed during the period of the study, and their possible relevance to influenzal events in the human population. None of the sera tested had antibody to swine influenza strains (HSw1N1).

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Swine influenza A virus subtypes circulating in Brazilian commercial pig herds from 2012 to 2019

Brazilian Journal of Microbiology ◽

10.1007/s42770-021-00550-y ◽

2021 ◽

Author(s):

Ana Luiza Soares Fraiha ◽

Ana Carolina Diniz Matos ◽

João Luis Reis Cunha ◽

Beatriz Senra Álvares da Silva Santos ◽

Maria Vitória Chaves Peixoto ◽

...

Keyword(s):

Influenza A Virus ◽

Influenza A ◽

Swine Influenza ◽

Swine Influenza A Virus

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Prospective studies of swine influenza A virus epidemiology on swine farms, China

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2020.09.1388 ◽

2020 ◽

Vol 101 ◽

pp. 535

Author(s):

G. Gray ◽

L.K. Borkenhagen ◽

G.-L. Wang ◽

M.-J. Ma

Keyword(s):

Influenza A Virus ◽

Prospective Studies ◽

Influenza A ◽

Swine Influenza ◽

Swine Farms ◽

Swine Influenza A Virus

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Tissue tropisms opt for transmissible reassortants during avian and swine influenza A virus co-infection in swine

PLoS Pathogens ◽

10.1371/journal.ppat.1007417 ◽

2018 ◽

Vol 14 (12) ◽

pp. e1007417 ◽

Cited By ~ 8

Author(s):

Xiaojian Zhang ◽

Hailiang Sun ◽

Fred L. Cunningham ◽

Lei Li ◽

Katie Hanson-Dorr ◽

...

Keyword(s):

Influenza A Virus ◽

Influenza A ◽

Swine Influenza ◽

Tissue Tropisms ◽

Swine Influenza A Virus

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Nested RT-PCR method for the detection of European avian-like H1 swine influenza A virus

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(15)61092-9 ◽

2016 ◽

Vol 15 (5) ◽

pp. 1095-1102

Author(s):

Yan-di WEI ◽

Xing-yao PEI ◽

Yuan ZHANG ◽

Chen-fang YU ◽

Hong-lei SUN ◽

...

Keyword(s):

Influenza A Virus ◽

Influenza A ◽

Swine Influenza ◽

Rt Pcr ◽

Pcr Method ◽

Swine Influenza A Virus

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Kinetics of single and dual simultaneous infection of pigs with swine influenza A virus and porcine reproductive and respiratory syndrome virus

Journal of Veterinary Internal Medicine ◽

10.1111/jvim.15832 ◽

2020 ◽

Vol 34 (5) ◽

pp. 1903-1913

Author(s):

Małgorzata Pomorska‐Mól ◽

Katarzyna Podgórska ◽

Ewelina Czyżewska‐Dors ◽

Hanna Turlewicz‐Podbielska ◽

Maciej Gogulski ◽

...

Keyword(s):

Influenza A Virus ◽

Influenza A ◽

Swine Influenza ◽

Respiratory Syndrome Virus ◽

Simultaneous Infection ◽

Kinetics Of ◽

Swine Influenza A Virus

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Objective pathogen monitoring in nursery and finisher pigs by monthly laboratory diagnostic testing

Porcine Health Management ◽

10.1186/s40813-020-00161-3 ◽

2020 ◽

Vol 6 (1) ◽

Author(s):

Nicole B. Goecke ◽

Maja Kobberø ◽

Thomas K. Kusk ◽

Charlotte K. Hjulsager ◽

Ken Steen Pedersen ◽

...

Keyword(s):

Real Time ◽

San Francisco ◽

Influenza A Virus ◽

High Throughput ◽

Real Time Pcr ◽

Influenza A ◽

Clinical Signs ◽

Swine Influenza ◽

Porcine Cytomegalovirus ◽

Swine Influenza A Virus

Abstract Background Infectious diseases are of great economic importance in commercial pig production, causing both clinical and subclinical disease, with influence on welfare, productivity, and antibiotic use. The causes of these diseases are often multifactorial and laboratory diagnostics are seldom routinely performed. The aim of the present study was to explore the benefits of monthly pathogen monitoring in nursery and finisher herds and to examine association between laboratory results and observed clinical signs, including coughing and diarrhoea. Three monthly samplings were conducted in three different age groups in six nursery and four finisher production units. For each unit, two pens were randomly selected in each age group and evaluated for coughing and diarrhoea events. Furthermore, faecal sock and oral fluid samples were collected in the selected pens and analysed for 18 respiratory and enteric viral and bacterial pathogens using the high-throughput real-time PCR BioMark HD platform (Fluidigm, South San Francisco, USA). Results In total, 174 pens were sampled in which eight coughing events and 77 diarrhoeic events were observed. The overall findings showed that swine influenza A virus, porcine circovirus 2, porcine cytomegalovirus, Brachyspira pilosicoli, Lawsonia intracellularis, Escherichia coli fimbria types F4 and F18 were found to be prevalent in several of the herds. Association between coughing events and the presence of swine influenza A virus, porcine cytomegalovirus (Cq ≤ 20) or a combination of these were found. Furthermore, an association between diarrhoeic events and the presence of L. intracellularis (Cq ≤ 24) or B. pilosicoli (Cq ≤ 26) was found. Conclusions The use of high-throughput real-time PCR analysis for continuous monitoring of pathogens and thereby dynamics of infections in a pig herd, provided the veterinarian and farmer with an objective knowledge on the distribution of pathogens in the herd. In addition, the use of a high-throughput method in combination with information about clinical signs, productivity, health status and antibiotic consumption, presents a new and innovative way of diagnosing and monitoring pig herds and even to a lower cost than the traditional method.

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