Anatomical and Physiological Effects of Phytohormones on Adventitious Roots Development in Larix kaempferi × L. olgensis

AbstractAdventitious root development is crucial for cutting propagation in hybrid larch. In the present study, semilignified cuttings of Larix kaempferi × L. olgensis clones were used to study the morphological and anatomical changes during the different development stages and compare the changes in contents of endogenous phytohormone in two clones with evidently different rooting capacity (25-5 and 23-12), and the effects of exogenous Indole-3-Butyric Acid (IBA) on phytohormone content of a very low native rooting capacity clone (8-9) during the development of adventitious roots. The results showed that the development of adventitious root in semi-lignified cuttings involves three key stages: root cell dedifferentiation and division (14-18 DAC); meristem formation and development (25-35 DAC); root formation and elongation (50-55 DAC). The endogenous phytohormones play a key role during adventitious root development. For 25-5 and 23-12, the level of endogenous phytohormones, especially the ABA content, has an important effect on rooting capacity. For the effects of IBA on 8-9, except lower ABA content, the temporary peak of IAA on the 1 DAC was important for initiating the rooting process. The ratio of (IAA + GA3 + ZR)/ABA can be used as an indicator of rooting capacity. The anatomical and physiological data described here for semi-lignified cuttings of L. kaempferi × L. olgensis clones improves our knowledge of adventitious roots development. Our study will also benefit future research on examining the molecular mechanisms of the adventitious roots development in hybrid larch.

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Transcriptome and proteome profiling of adventitious root development in hybrid larch (Larix kaempferi × Larix olgensis)

BMC Plant Biology ◽

10.1186/s12870-014-0305-4 ◽

2014 ◽

Vol 14 (1) ◽

Cited By ~ 17

Author(s):

Hua Han ◽

Xiaomei Sun ◽

Yunhui Xie ◽

Jian Feng ◽

Shougong Zhang

Keyword(s):

Root Development ◽

Adventitious Root ◽

Larix Kaempferi ◽

Larix Olgensis ◽

Hybrid Larch ◽

Proteome Profiling ◽

Adventitious Root Development

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Characterization of Adventitious Root Development in Sweetpotato

HortScience ◽

10.21273/hortsci.44.3.651 ◽

2009 ◽

Vol 44 (3) ◽

pp. 651-655 ◽

Cited By ~ 30

Author(s):

Arthur Q. Villordon ◽

Don R. La Bonte ◽

Nurit Firon ◽

Yanir Kfir ◽

Etan Pressman ◽

...

Keyword(s):

Root Development ◽

Adventitious Root ◽

Adventitious Roots ◽

Vascular Cambium ◽

Storage Root ◽

Storage Roots ◽

Adventitious Root Development ◽

Xylem Elements ◽

Storage Root Development

Adventitious roots of ‘Beauregard’ and ‘Georgia Jet’ sweetpotato were observed and anatomically characterized over a period of 60 days of storage root development. The majority of ‘Beauregard’ and ‘Georgia Jet’ adventitious roots sampled at 5 to 7 days after transplanting (DAT) possessed anatomical features (five or more protoxylem elements) associated with storage root development. The majority of ‘Beauregard’ (86%) and ‘Georgia Jet’ (89%) storage roots sampled at 60 to 65 DAT were traced directly to adventitious roots extant at 5 to 7 DAT. The two varieties, however, differed in the timing in which regular and anomalous cambia were formed. Regular vascular cambium development, i.e., initiation and completion, was observed in both varieties at 19 to 21 DAT. Formation of complete regular vascular cambium was negligible for ‘Beauregard’ (4%) in comparison with ‘Georgia Jet’ (32%) at 26 to 28 DAT. However, anomalous cambia development adjacent to xylem elements was greater in ‘Beauregard’ (30%) in comparison with ‘Georgia Jet’ (13%). Nearly 40% to 50% of samples in both varieties showed extensive lignification in the stele region. At 32 to 35 DAT, 62% to 70% of the adventitious roots for both varieties had either been initiated (developed anomalous cambium) or were lignified. The remaining adventitious roots showed intermediate stages of vascular cambium development. The adventitious root count increased up to 19 to 21 DAT and then remained constant up to 32 to 35 DAT. These accumulated results suggest that the initial stages of adventitious root development are critical in determining storage root set in sweetpotato.

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Highly Successful Adventitious Root Formation of Zamia L. Stem Cuttings Exhibits Minimal Response to Indole-3-Butyric Acid

HortScience ◽

10.21273/hortsci15212-20 ◽

2020 ◽

Vol 55 (9) ◽

pp. 1463-1467 ◽

Cited By ~ 3

Author(s):

Benjamin E. Deloso ◽

Anders J. Lindström ◽

Frank A. Camacho ◽

Thomas E. Marler

Keyword(s):

Butyric Acid ◽

Root Development ◽

Adventitious Root ◽

Adventitious Roots ◽

Root Formation ◽

Adventitious Root Formation ◽

The Other ◽

Control Group ◽

Stem Cuttings ◽

Adventitious Root Development

The influences of indole-3-butyric acid (IBA) concentrations of 0–30 mg·g−1 on the success and speed of adventitious root development of Zamia furfuracea L.f. and Zamia integrifolia L.f. stem cuttings were determined. Root formation success for both species was greater than 95%. The IBA concentrations did not influence the speed of root development for Z. furfuracea, but the Z. integrifolia cuttings that received IBA concentration of 3 mg·g−1 generated adventitious roots more slowly than the cuttings in the control group. The ending dry weights of the stems, leaves, and roots were not influenced by IBA concentration for either species. Our results indicated that adventitious root formation on stem cuttings of these two Zamia species is successful without horticultural application of IBA. Additional IBA studies are needed on the other 300+ cycad species, especially those that are in a threatened category.

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Transcription factor LkWOX4 is involved in adventitious root development in Larix kaempferi

Gene ◽

10.1016/j.gene.2020.144942 ◽

2020 ◽

Vol 758 ◽

pp. 144942 ◽

Cited By ~ 1

Author(s):

Hongming Wang ◽

Yunhui Xie ◽

Wusheng Liu ◽

Guiyun Tao ◽

Chao Sun ◽

...

Keyword(s):

Transcription Factor ◽

Root Development ◽

Adventitious Root ◽

Larix Kaempferi ◽

Adventitious Root Development

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Influence of phenyl tryptophyl ether on adventitious root development in bean cuttings

Canadian Journal of Botany ◽

10.1139/b83-166 ◽

1983 ◽

Vol 61 (5) ◽

pp. 1548-1549 ◽

Cited By ~ 1

Author(s):

Bruce E. Haissig

Keyword(s):

Acetic Acid ◽

Phaseolus Vulgaris ◽

Root Development ◽

Adventitious Root ◽

Adventitious Root Development ◽

Indole 3 Acetic Acid

Rooting trials with bean (Phaseolus vulgaris cv. Top Crop L.) tested the effects of phenyl tryptophyl ether (PTRE) in comparison with tryptophol (TR, indole-3-ethanol), indole-3-acetic acid (IAA), and no treatment. PTRE was synthesized from tryptophol and phenol. Results indicated that IAA treatment enhanced primordium development significantly more than any other treatment that was tested. Effects of TR and PTRE treatment on primordium development were about equal. Results suggested that PTRE is not an auxin or is only a weak auxin in comparison with IAA.

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