<p><i>Cyphostemma adenocaule </i>(Steud.
ex A. Rich.) is one of the specie plant that belongs to the family vitacea. In this
study, Trilinolein was isolated and characterized from the methanol root extract
of the plant. Column chromatography over silica gel granules as the stationary
phase and eluted with a mobile phase mixture of n-Hex-EtA; EtA-CHCL3 and CHCL<sub>3</sub>-MeOH
with gradient increasing polarity, followed by a second column using
saphadex-LH20 and 100% MeOH as stationary and mobile phase vehicle
respectively. TLC was developed with EtA 15: CHCL3<sub> </sub>8: MeOH 4: H<sub>2</sub>O
1 as solvent system; sprayed with 10% H<sub>2</sub>SO<sub>4 </sub>,Vanillin-sulphuric acid, and/ or Polyethylene glycol
PEG and heat for spot detection and confirmation of bioactive principles. Compound
CA1 was obtained and purified with CHCL3 to give a yellow semi-solid compound
(0.23g). The <sup>1</sup>H-NMR spectra showed 9 different signals; a signal
peak of a glycerol (-C<b>H<sub>2</sub></b>OCOR-)
moiety on the first α-C chain and on the
third αʹ-C at 4.143-4.187ppm and 4.296-4.325ppm
respectively, while that of a β glycerol (-C<b>H</b>COR-) at 5.286ppm. Signals of an allylic methylene group at
2.023-2.035ppm, Olefenic hydrogen group at signal peak of 5.362ppm and a
diallylic methylene group at signal 2.790ppm were also observed. In the <sup>13</sup>C
NMR spectra of compound CA1, 57 carbon atoms where observed, multiple signals
overlapping at a range of 14.13-34.21ppm corresponding to the aliphatic CH3 (<b>C18</b>), CH2 (<b>C2, C3, C4, C5, C6, C7, C15, C16, and C17</b>) and allylic (<b>C8, C14</b>) carbon atoms. Signals at
127.90-130.24ppm were assigned to the olefienic C atoms (<b>C9, C10, C12</b>, and <b>C13</b>)
while signal of 172.87ppm and 173.32ppm were assigned to the carbonyl (<b>C</b>=O) carbon atoms (<b>C1 </b>and<b> C2</b>) respectively
(Table 2). </p>
<p>Analysis
with DEPT-135, H-H COSY, HMBC and HSQC assignments of CA1 augments assignment of
signals made for CA1 from <sup>1</sup>H-NMR and <sup>13</sup>C-NMR and corresponded
to that of Trilinolein <u>(<a href="https://pubchem.ncbi.nlm.nih.gov/#query=C57H98O6">C<sub>57</sub>H<sub>98</sub>O<sub>6</sub></a>,
</u>MW 879.4 g/mol). The isolated compound
was positive for the acrolein test for triglycerides; fat & oil and had an
IC<sub>50</sub> of 46.08µg/ml radical scavenging activity.</p>
A Natural Triglyceride from the Methanol Root Extract of Cyphostemma Adenocaule (Steud. Ex A. Rich.) Wild & R.B.Drumm
