A High Potential Iron Sulfur Protein of the Purple Sulfur Bacterium Thiocapsa roseopersicina

1980 ◽  
Vol 35 (1-2) ◽  
pp. 150-153 ◽  
Author(s):  
Ulrich Fischer
Keyword(s):  
Heme Iron ◽  
High Potential ◽  
Reduced Form ◽  
Sephadex Column ◽  
Purple Sulfur Bacterium ◽  
Thiocapsa Roseopersicina ◽  
Non Heme Iron ◽  
Iron Sulfur ◽  
Sulfur Protein ◽  
Iron Sulfur Protein

Abstract Thiocapsa roseopersicina, High Potential Iron Sulfur Pro­ tein (HIPIP), Phototrophic Bacteria High potential iron sulfur protein (HIPIP) from the purple sulfur bacterium Thiocapsa roseopersicina was isolated in the oxidized and reduced form by DEAE-and Sephadex column chromatography (best purity index A 282 nm red/A 385 nm red. = 3.78). The enzyme contains non-heme iron and acid-labile sulfide in an equimolar ratio. The enzyme shows no aconitase activity.

Molecular Properties of High Potential Iron Sulfur Protein of Chromatium warmingii

1983 ◽  
Vol 38 (11-12) ◽  
pp. 968-971 ◽  
Author(s):  
Ursula Wermter ◽  
Ulrich Fischer
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecylsulfate ◽  
Gel Filtration ◽  
Exchange Chromatography ◽  
High Potential ◽  
Reduced Form ◽  
Purple Sulfur Bacterium ◽  
Iron Sulfur ◽  
Sulfur Protein ◽  
Iron Sulfur Protein

High potential iron sulfur protein (HIPIP) of the purple sulfur bacterium Chromatium warmingii was purified to homogeneity by ion exchange chromatography, gel filtration and ammonium sulfate fractionation. The acidic protein was isolated in the reduced form. The best purity index (A280/A388) obtained was 2.52, and 3.8 μmol of the protein was isolated out of 100 g wet cell material. The molecular weights estimated by sodium dodecylsulfate polyacrylamide gel electrophoresis and gel filtration through Sephacryl S-200 were 8 900 and 10 500, respec­tively. The protein has an isoelectric point at pH 3.6 for the reduced form and at pH 3.8 for the oxidized form, and a midpoint redox potential of +355 mV. One mol of HIPIP contains 4 mol nonheme iron and 4 mol acid-labile sulfur

scholarly journals Two distinct binding sites for high potential iron-sulfur protein and cytochrome c on the reaction center-bound cytochrome of Rubrivivax gelatinosus.

2004 ◽  
Vol 279 (44) ◽  
pp. 46350
Author(s):  
Jean Alric ◽  
Makoto Yoshida ◽  
Kenji V.P. Nagashima ◽  
Rainer Hienerwadel ◽  
Pierre Parot ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Reaction Center ◽  
Binding Sites ◽  
High Potential ◽  
Iron Sulfur ◽  
Sulfur Protein ◽  
Iron Sulfur Protein ◽  
Rubrivivax Gelatinosus

scholarly journals 1H, 13C and 15N assignment of the paramagnetic high potential iron–sulfur protein (HiPIP) PioC from Rhodopseudomonas palustris TIE-1

2020 ◽  
Vol 14 (2) ◽  
pp. 211-215 ◽  
Author(s):  
Inês B. Trindade ◽  
Michele Invernici ◽  
Francesca Cantini ◽  
Ricardo O. Louro ◽  
Mario Piccioli
Keyword(s):  
Rhodopseudomonas Palustris ◽  
High Potential ◽  
Iron Sulfur ◽  
Sulfur Protein ◽  
Iron Sulfur Protein

Kinetics of the oxidation of high-potential iron-sulfur protein from Chromatium by ferrocenium derivatives

1987 ◽  
Vol 26 (13) ◽  
pp. 2058-2062 ◽  
Author(s):  
J. R. Pladziewicz ◽  
A. J. Abrahamson ◽  
R. A. Davis ◽  
M. D. Likar
Keyword(s):  
High Potential ◽  
Iron Sulfur ◽  
Sulfur Protein ◽  
Iron Sulfur Protein ◽  
Kinetics Of
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