Fluorescence Patterns of Chromatin and Cytoplasm by Hematoxylin Solutions

1983 ◽  
Vol 38 (1-2) ◽  
pp. 153-155 ◽  
Author(s):  
J. C. Stockert ◽  
M. L. Molero ◽  
R. H. Espelosin

Hematoxylin, Fluorescence, Fluorochrom es Treatments o f chicken blood and Ehrlich ascites tumor smears with hematoxylin solutions give a fluorescence reaction in chromatin, basophilic cytoplasm and leukocyte granules. In these structures the fluorescence emission in­ creases upon dye aging and prolonged staining times. We present a preliminar spectral analysis suggesting the possibility to employ hematoxylin as a fluorochrome.

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