Characterization of Volatile Constituents from Heterotrophic Cell Suspension Cultures of Ruta graveolens

Heterotrophic cell suspension cultures of Ruta graveolens were established by reversion of photomixotrophic cultures without any change in the chemical composition of the growth medium. The heterotrophic cultures were qualitatively and quantitatively analyzed by gas- chromatography and mass spectroscopy for volatile compounds. The terpenoid hydrocarbons geijerene and pregeijerene, C6-C8 ketones, acetic acid n-butylester and a series of aliphatic C4-C9 primary and secondary alcohols were found as main constituents. Two isomeric sabinene hydrates were also isolated as new constituents of rue cells. The data are discussed in comparison to results obtained with photomixotrophic cell suspension cultures.

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Characterization of Volatile Constituents from Photomixotrophic Cell Suspension Cultures of Ruta graveolens

Zeitschrift für Naturforschung C ◽

10.1515/znc-1984-0603 ◽

1984 ◽

Vol 39 (6) ◽

pp. 525-530 ◽

Cited By ~ 9

Author(s):

Friednch Drawert ◽

Ralf G. Berger ◽

Rolf Godelmann ◽

Susanne Collin ◽

Wolfgang Barz

Keyword(s):

Acetic Acid ◽

Cell Suspension ◽

Cell Suspension Cultures ◽

Callus Cultures ◽

Suspension Cultures ◽

Ruta Graveolens ◽

Straight Chain ◽

Methylbutyric Acid ◽

Aliphatic Esters

Photomixotrophic cell suspension cultures of Ruta graveolens were qualitatively and quantitatively analyzed by gaschromatography and mass spectroscopy for volatile compounds. The terpenoid hydrocarbons geijerene and pregeijerene, the C9-C13 methylketones and a series of aliphatic esters, respectively, were found as main constituents. The esters consisted of acetic acid, 2-methylbutyric acid and 3-methylbutyric acid which were esterified with straight chain or branched C8-C11 alcohols. The data are discussed in comparison to previous studies on callus cultures.

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Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.]

Notulae Scientia Biologicae ◽

10.15835/nsb447963 ◽

2012 ◽

Vol 4 (4) ◽

pp. 49-54 ◽

Cited By ~ 1

Author(s):

Sara KHOSRAVINIA ◽

Seyed Mahdi ZIARATNIA ◽

Abdolreza BAGHERI ◽

Ghadir RAJABZADEH ◽

Seyed Hassan MARASHI

Keyword(s):

Gas Chromatography ◽

Acetic Acid ◽

Cell Suspension ◽

Supercritical Fluid ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Naphthalene Acetic Acid ◽

Chromatography Analysis ◽

Bunium Persicum ◽

Dichlorophenoxy Acetic Acid

The cell suspension culture and seed samples of Bunium persicum were extracted by supercritical fluid, hydrodistillation and solvent methods and analyzed by Gas Chromatography. In this study to compare the different methods of extractions, cuminaldehyde was targeted as one of the Black zira essential oil constitute. For callus induction the germinated seeds were cultured as explants on Murashige and Skoog medium supplemented with 2 mg/l 2,4-dichlorophenoxy acetic acid and 0.5 mg/l kinetin (treatment A) as well as 2 mg/l ?-naphthalene acetic acid and 0.5 mg/l 6-benzyl aminopurine (treatment B) and followed by cells suspension cultures establishment for the first time. The results of cell culture showed that cells from treatment B have a growth rate higher than A. All extracts were dissolved in 1 ml hexane and analyzed by Gas Chromatography. According to the Gas Chromatography analysis, cuminaldehyde was not detected in the supercritical fluid samples, while it was present in hydrodistillation and solvent extract. Cuminaldehyde percentage in cell and seed solvent extracts was 4.65% and 18.61% respectively. Gas Chromatography results also showed that no cuminaldehyde is present in media extracts, means no cuminaldehyde has been secreted into the medium.

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