scholarly journals Solubilization and Characterization of a Membrane-Bound Auxin-Binding Protein from Cell Suspension Cultures of Nicotiana tabacum

1988 ◽  
Vol 88 (3) ◽  
pp. 685-689 ◽  
Author(s):  
Chiharu Nakamura ◽  
Hajime Ono
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Suspension ◽  
Binding Protein ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Auxin Binding ◽  
Membrane Bound ◽  
Auxin Binding Protein

scholarly journals Further Characterization of Expression of Auxin-Induced Genes in Tobacco (Nicotiana tabacum) Cell-Suspension Cultures

1993 ◽  
Vol 102 (2) ◽  
pp. 513-520 ◽  
Author(s):  
KJM. Boot ◽  
B. J. van der Zaal ◽  
J. Velterop ◽  
A. Quint ◽  
A. M. Mennes ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Induced Genes

Isolation and characterization of an UDPG-dependent glucosyltransferase activity from Rauwolfia serpentina Benth. cell suspension cultures

1994 ◽  
Vol 72 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Ralf Lutterbach ◽  
Carl Michael Ruyter ◽  
Joachim Stöckigt
Keyword(s):  
Enzyme Activity ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Rauwolfia Serpentina ◽  
Isolation And Characterization ◽  
Membrane Bound ◽  
Plant Families

From cell suspension cultures of Rauwolfia serpentina Benth. a new enzyme activity was isolated and its properties determined. The enzyme is a soluble protein and catalyzes the transfer of a glucose moiety from UDPG to a wide variety of phenolic compounds with p-nitrophenol as one of the best substrates (Km = 1.21 mM, UDPG = 0.54 mM). In contrast to the membrane-bound UDPG: vomilenine-21-OH-β-D-glucosyltransferase from Rauwolfia serpentina cells, this enzyme is not able to glucosylate indole alkaloids. The enzyme activity has been detected in 14 callus cultures belonging to 10 different plant families.

scholarly journals Correlation between Auxin Resistance and the Lack of a Membrane-Bound Auxin Binding Protein and a Root-Specific Peroxidase in Nicotiana tabacum

1988 ◽  
Vol 88 (3) ◽  
pp. 845-849 ◽  
Author(s):  
Chiharu Nakamura ◽  
Hendrik-Jan Van Telgen ◽  
Albert M. Mennes ◽  
Hajime Ono ◽  
Kees R. Libbenga
Keyword(s):  
Nicotiana Tabacum ◽  
Binding Protein ◽  
Auxin Binding ◽  
Auxin Resistance ◽  
Membrane Bound ◽  
Auxin Binding Protein

scholarly journals Biotransformation of Cyclodextrine-Complexed Semisynthetic Betulin Derivatives by Plant Cells

Planta Medica ◽  
2018 ◽  
Vol 84 (09/10) ◽  
pp. 743-748
Author(s):  
Suvi Häkkinen ◽  
Heli Nygren ◽  
Natalia Maiorova ◽  
Raisa Haavikko ◽  
Sami Alakurtti ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Plant Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Water Soluble ◽  
Betulonic Acid ◽  
Poorly Water Soluble ◽  
Betulin Derivatives

AbstractIn this study, three semisynthetic betulonic acid-based compounds, 20(29)-dihydrolup-2-en[2,3-d]isoxazol-28-oic acid, 1-betulonoylpyrrolidine, and lupa-2,20(29)-dieno[2,3-b]pyrazin-28-oic acid, were studied in biotransformation experiments using Nicotiana tabacum and Catharanthus roseus cell suspension cultures. Biotransformation was performed using cyclodextrin to aid dissolving poorly water-soluble substrates. Several new derivatives were found, consisting of oxidized and glycosylated (pentose- and hexose-conjugated) products.

Production and characterization of human CTLA4Ig expressed in transgenic rice cell suspension cultures

2007 ◽  
Vol 51 (2) ◽  
pp. 293-302 ◽  
Author(s):  
Song-Jae Lee ◽  
Cheon-Ik Park ◽  
Mi-Young Park ◽  
Hahn-Sun Jung ◽  
Wuk-Sang Ryu ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Transgenic Rice ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

Characterization of Volatile Constituents from Photomixotrophic Cell Suspension Cultures of Ruta graveolens

1984 ◽  
Vol 39 (6) ◽  
pp. 525-530 ◽  
Author(s):  
Friednch Drawert ◽  
Ralf G. Berger ◽  
Rolf Godelmann ◽  
Susanne Collin ◽  
Wolfgang Barz
Keyword(s):  
Acetic Acid ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Ruta Graveolens ◽  
Straight Chain ◽  
Methylbutyric Acid ◽  
Aliphatic Esters

Photomixotrophic cell suspension cultures of Ruta graveolens were qualitatively and quantita­tively analyzed by gaschromatography and mass spectroscopy for volatile compounds. The terpenoid hydrocarbons geijerene and pregeijerene, the C9-C13 methylketones and a series of aliphatic esters, respectively, were found as main constituents. The esters consisted of acetic acid, 2-methylbutyric acid and 3-methylbutyric acid which were esterified with straight chain or branched C8-C11 alcohols. The data are discussed in comparison to previous studies on callus cultures.

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