scholarly journals Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.]

2012 ◽  
Vol 4 (4) ◽  
pp. 49-54 ◽  
Author(s):  
Sara KHOSRAVINIA ◽  
Seyed Mahdi ZIARATNIA ◽  
Abdolreza BAGHERI ◽  
Ghadir RAJABZADEH ◽  
Seyed Hassan MARASHI
Keyword(s):  
Gas Chromatography ◽  
Acetic Acid ◽  
Cell Suspension ◽  
Supercritical Fluid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Naphthalene Acetic Acid ◽  
Chromatography Analysis ◽  
Bunium Persicum ◽  
Dichlorophenoxy Acetic Acid

The cell suspension culture and seed samples of Bunium persicum were extracted by supercritical fluid, hydrodistillation and solvent methods and analyzed by Gas Chromatography. In this study to compare the different methods of extractions, cuminaldehyde was targeted as one of the Black zira essential oil constitute. For callus induction the germinated seeds were cultured as explants on Murashige and Skoog medium supplemented with 2 mg/l 2,4-dichlorophenoxy acetic acid and 0.5 mg/l kinetin (treatment A) as well as 2 mg/l ?-naphthalene acetic acid and 0.5 mg/l 6-benzyl aminopurine (treatment B) and followed by cells suspension cultures establishment for the first time. The results of cell culture showed that cells from treatment B have a growth rate higher than A. All extracts were dissolved in 1 ml hexane and analyzed by Gas Chromatography. According to the Gas Chromatography analysis, cuminaldehyde was not detected in the supercritical fluid samples, while it was present in hydrodistillation and solvent extract. Cuminaldehyde percentage in cell and seed solvent extracts was 4.65% and 18.61% respectively. Gas Chromatography results also showed that no cuminaldehyde is present in media extracts, means no cuminaldehyde has been secreted into the medium.

Characterization of Volatile Constituents from Heterotrophic Cell Suspension Cultures of Ruta graveolens

1986 ◽  
Vol 41 (9-10) ◽  
pp. 809-812 ◽  
Author(s):  
Michael Jordan ◽  
Claus H. Rolfs ◽  
Wolfgang Barz ◽  
Ralf G. Berger ◽  
Hubert Kollmannsberger ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Acetic Acid ◽  
Chemical Composition ◽  
Cell Suspension ◽  
Mass Spectroscopy ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Secondary Alcohols ◽  
Ruta Graveolens

Heterotrophic cell suspension cultures of Ruta graveolens were established by reversion of photomixotrophic cultures without any change in the chemical composition of the growth medium. The heterotrophic cultures were qualitatively and quantitatively analyzed by gas- chromatography and mass spectroscopy for volatile compounds. The terpenoid hydrocarbons geijerene and pregeijerene, C6-C8 ketones, acetic acid n-butylester and a series of aliphatic C4-C9 primary and secondary alcohols were found as main constituents. Two isomeric sabinene hydrates were also isolated as new constituents of rue cells. The data are discussed in comparison to results obtained with photomixotrophic cell suspension cultures.

Degradation of Phenoxyalkylcarboxylic Acids by White Clover (Trifolium repens) Cell Suspensions

Weed Science ◽  
1979 ◽  
Vol 27 (4) ◽  
pp. 389-391 ◽  
Author(s):  
A. E. Smith ◽  
T. H. Oswald
Keyword(s):  
Acetic Acid ◽  
Cell Suspension ◽  
Butyric Acid ◽  
White Clover ◽  
Trifolium Repens ◽  
Herbicide Tolerance ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Populations ◽  
Dichlorophenoxy Acetic Acid

Cell suspension cultures of white clover (Trifolium repensL. ‘Regal Ladino’) were treated with 2,4-D [(2,4-dichlorophenoxy)acetic acid], 2,4-DB [4-(2,4-dichlorophenoxy)butyric acid], 2,4,5-T [(2,4,5-trichlorophenoxy)acetic acid], 2,4,5-TP [2-(2,4,5-trichlorophenoxy) propionic acid], and 2,4,5-TB [4-(2,4,5-trichlorophenoxy)butyric acid]. Cell population densities were monitored throughout the treatment period and herbicide remaining in the cells and culture medium was extracted and quantified at the termination of the treatment. Herbicide tolerance increased in cell populations which were conditioned by pretreatment with 2,4-DB and 2,4,5-T. However, 2,4-DB was rapidly degraded by all cell populations regardless of pretreatment. White clover cell suspension cultures treated with 2,4-D, 2,4-DB, 2,4,5-T, 2,4,5-TP and 2,4,5-TB metabolized the homologs, according to the following sequence: butyric acidåpropionic acidåacetic acid homologs. There was no difference in the rate of degradation of similar homologs of the 2,4-dichloro- and 2,4,5-trichloro- analogs.

Herbicide tolerance developed in cell suspension cultures of perennial white clover

1977 ◽  
Vol 55 (10) ◽  
pp. 1351-1358 ◽  
Author(s):  
T. H. Oswald ◽  
A. E. Smith ◽  
D. V. Phillips
Keyword(s):  
Acetic Acid ◽  
Cell Suspension ◽  
White Clover ◽  
Herbicide Tolerance ◽  
Cell Suspension Cultures ◽  
Fold Increase ◽  
Suspension Cultures ◽  
Selection Procedures ◽  
Trifolium Repens L ◽  
Dichlorophenoxy Acetic Acid

Cell suspension cultures of perennial white clover (Trifolium repens L. cv. Regal Ladino) were selected for tolerance to the phenoxy analogs (2,4-dichlorophenoxy)acetic acid (2.4-D), 2,4,5-trichlorophenoxy)acetic acid (2,4,5-T), and 4-(2,4-dichlorophenoxy)butyric acid (2,4-DB). Cells demonstrating up to 6.2-fold increase in tolerance to 4 μg/ml 2,4-D, 7.7-fold increase in tolerance to 8 μg/ml 2,4,5-T, and 3.0-fold increase in tolerance to 2 μg/ml 2,4-DB were obtained within 5 days under the selection procedures. The selection pressures were the phenoxy analogs applied at phytotoxic levels. Whereas the cells that survived each pressure subsequently demonstrated tolerance to all analogs, the cells selected with 2,4-DB as the pressure were more tolerant to all three analogs than were cells selected with 2,4-D or 2,4,5-T. Tolerance, thus gained, was transmitted through succeeding asexually propagated cell generations.

Characterization of Volatile Constituents from Photomixotrophic Cell Suspension Cultures of Ruta graveolens

1984 ◽  
Vol 39 (6) ◽  
pp. 525-530 ◽  
Author(s):  
Friednch Drawert ◽  
Ralf G. Berger ◽  
Rolf Godelmann ◽  
Susanne Collin ◽  
Wolfgang Barz
Keyword(s):  
Acetic Acid ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Ruta Graveolens ◽  
Straight Chain ◽  
Methylbutyric Acid ◽  
Aliphatic Esters

Photomixotrophic cell suspension cultures of Ruta graveolens were qualitatively and quantita­tively analyzed by gaschromatography and mass spectroscopy for volatile compounds. The terpenoid hydrocarbons geijerene and pregeijerene, the C9-C13 methylketones and a series of aliphatic esters, respectively, were found as main constituents. The esters consisted of acetic acid, 2-methylbutyric acid and 3-methylbutyric acid which were esterified with straight chain or branched C8-C11 alcohols. The data are discussed in comparison to previous studies on callus cultures.

scholarly journals Callus induction and chemical characterization of cell suspension cultures of jojoba (Simmondsia chinensis L.)

2021 ◽  
Vol 6 (3) ◽  
pp. 5
Author(s):  
Muhammad Akram ◽  
Faheem Aftab
Keyword(s):  
Cell Viability ◽  
Cell Suspension ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Counting ◽  
Naphthalene Acetic Acid ◽  
Simmondsia Chinensis ◽  
Triphenyltetrazolium Chloride ◽  
Efficient System

Jojoba (Simmondsia chinensis L.) oil is also known as liquid wax or fixed oil. It is an important metabolite of jojoba having commercial importance in cosmetics as well as a potential biofuel source. We presented an efficient system for in vitro establishment of cell suspension cultures (CSC) from proliferating friable calluses. For this purpose, cotyledon, internode, and leaf explants were cultured on MS medium + 1, 2, 4, 6, 8 or 10 µM 2, 4-Dichlorophenoxyacetic acid (2, 4-D), α-Naphthalene acetic acid (NAA) alone or in combination with 1 or 2 µM N6-benzylaminopurine (BAP) or Kinetin. Results demonstrated that 100% healthy, friable and variegated calluses were obtained on 8 µM, 10 µM 2, 4-D or 2, 4-D 10 µM + 2 µM BAP and represented as callus lines (CL) CL-1, CL-2 or CL-3, respectively, after 38 days. One-gram callus tissue per CL was then immersed in the respective liquid medium and agitated on an orbital shaker at 60-70 rpm under the growth room conditions (25 ± 2 °C, 16 h light period) for the preparation of CSC. After 15 days, CSC was sieved and large clumps were removed. Growth measurement of CSC was determined by cell counting, packed cell volume (PCV) and cell viability. The highest number of viable cells was obtained at 2.57 OD with CL-3, where PCV was highest (0.35 ml) on CL-1 of 38 days old calluses. 2,3,5-Triphenyltetrazolium chloride was a reliable approach for the determination of cell viability of CSC.

The effect of 2,4-D and kinetin on peroxidase activity and isoenzyme pattern in cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1976 ◽  
Vol 54 (16) ◽  
pp. 1857-1867 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Growth Regulators ◽  
Peroxidase Activity ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Peroxidase Isoenzymes ◽  
Cotyledon Cell ◽  
Cell Growth And Differentiation ◽  
Dichlorophenoxy Acetic Acid

Cotyledon cell suspension cultures of Phaseolus vulgaris were grown in the presence and absence of the growth regulators (2,4-dichlorophenoxy)acetic acid (2,4-D) and kinetin. Peroxidase (EC 1.11.1.7) activity was at a minimum during the phase of cell division and at a maximum during the phase of cell expansion. Both the pattern and activity of peroxidase isoenzymes changed during the culture cycle.Cells cultured without growth regulators showed increased peroxidase activity and changed isoenzyme patterns. Certain peroxidase isoenzymes were only present or prominent during specific phases of the culture cycle.The electrophoretic mobilities of peroxidase isoenzymes detected in the medium were not the same as those of the cytoplasmic isoenzymes. Cell cultures grown with and without growth regulators showed different patterns of medium peroxidase activity.Results are discussed in relation to the correlation of peroxidase activity with cell wall expansion and the possible role of peroxidase in cell growth and differentiation.

scholarly journals Rosmarinic acid production in cell suspension cultures of Ehretia asperula Zollinger & Moritz

2022 ◽  
Vol 9 (1) ◽  
pp. 70-75
Author(s):  
Pham Thi My Tram ◽  
Ngo Ke Suong ◽  
Le Thi Thuy Tien
Keyword(s):  
Cell Suspension ◽  
Rosmarinic Acid ◽  
Rotation Speed ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Inoculum Size ◽  
Naphthalene Acetic Acid ◽  
Callus Line ◽  
Pharmaceutical Industries

Plant cell cultures provide an alternative means for producing secondary compounds in food, cosmetic and pharmaceutical industries. Ehretia asperula Zollinger & Moritzi is used as a traditional medicine for the treatment of liver detoxification, ulcers, tumors, inflammation and enhancing the body's resistance in Vietnam. The study was carried out to select suitable callus line for cell suspension cultures of E. asperula Zollinger & Moritzi and investigate the effects of inoculum size, rotation speed and naphthalene acetic acid (NAA) on the proliferation of cell suspension cultures. In addition, the influence of light intensity on the growth and rosmarinic acid (RA) biosynthesis of cell suspension was also surveyed. After 4 weeks of culture, the white to pale yellow friable callus expanded significantly with a fresh weight (FW) of 0.788 g and a high RA content of 2.062 mg/g FW. An appropriate medium for cell proliferation was the liquid B5 medium, which contained 30 g/l glucose, 0.1 mg/l benzyl adenine (BA) and 0.4 mg/l NAA. The results also demonstrated that a 1:20 ratio (w/v) inoculum size, darkness and rotation speed of 90 rpm were the optimal conditions for the proliferation and RA accumulation to 188.217 mg/l in 4 weeks of culture. These findings showed that E. asperula Zollinger & Moritzi cell suspension cultures could be a potential alternative approach for RA production in vitro.

The effect of 2,4-D and kinetin on the morphology, growth, and cytochemistry of peroxidase of cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1976 ◽  
Vol 54 (16) ◽  
pp. 1847-1856 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Peroxidase Activity ◽  
Morphological Characteristics ◽  
Enzyme Reaction ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Age ◽  
Cotyledon Cell ◽  
Dichlorophenoxy Acetic Acid

Cotyledon cell suspension cultures of Phaseolus vulgaris were grown in the presence and absence of the growth regulators (2,4-dichlorophenoxy)acetic acid (2,4-D) and kinetin. Omission of the regulators changed the growth and cell division and produced striking changes in morphological characteristics. Cytochemical studies of peroxidase activity showed that the enzymes were mainly cytoplasmic in young cells and mainly associated with wall in older cells. No apparent differences in localization of activity were detected between the treatments, but cells cultured without the regulators showed a much more intense enzyme reaction. The location and increase of peroxidase, with increasing cell age and cell complexity, are consistent with the view that peroxidase activity is involved in cell wall expansion and differentiation.

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